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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02818
  • pan locus tag?: SAUPAN006148000
  • symbol: SAOUHSC_02818
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02818
  • symbol: SAOUHSC_02818
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2596034..2597197
  • length: 1164
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGATTAATAGTAATATCAGATTATATATTCTAGGATTTTTAGCATTTTTCGCATCATTG
    ATTCAAAACATATACACGCCTATTATTCCGAGACTATATGACGATTTTCAAGTACCACTA
    TTTTGGATAAATGCGACAGTTGGCGGTTTTATATTTATCGTTGCAGTTATGCAAATTGTA
    CTTGGTAGAAGTATAGATTCTCGTGATTCGAAAAAAGTACTTTTAACGGGATTGGGAATA
    GTAATCATATCTAGCTTTATTTGTGCAGTGACACATAACTTTATATTATTTGCTATATCA
    AGGTTATTTCAAGCAATTGGTTGCGGAATTATTCCTCTCGTGACTTTAACTTTATTAGCC
    AAACTAAGTACAGATAATGGTAGAGCTCAAGCTATGGCGAACTATCAAATATTTTTATCA
    TGTGCACCAGCTTTGGCACCAATCTTAGGGAGTACTTTAGGTGCAAGATGGGATTATATT
    GGTATTTTTAGTTTTTTGCTCGTTATATCTATCGTATTATTTTTGATTATCTTTTTTATT
    GATATTCCAAATGTTGAAAAAGGGATTGTAAAATTAACTGAAAAAATTGAAGAAAAATAT
    TTGACCGATAAAGTGTTTATTACTTTAGTGACATTGGGATTTTTAATATTTTTTACGTAC
    TTTTCAATATTAGTTTATTTACCAACATTATTAAATAATACCTATGATATAGGCGTTGGA
    ATATCAGGAGTTTTGTTTTTACCTATTACTGTTAGTGTTATATTAGGAAGTTTATTTTAT
    AAGCGATTTTCAAAAAAATATAATGAAATAATGATACTTCGTGTTACTATAACTGGATTT
    GCTATATTTACGTTATTGTTTGGATGGTTAAACGAATCAAATGTAATTATATTATCAGTA
    ATTATATTTATATTAGGTACTTGTGTTGGTATTGTGCCAGCTTTGCTGTCTACTATAATT
    TCTAAAAGGTTTGAACATATAAAAGGGAAAGTGCTAGGTGTATTTAATTTTGTGAGATAT
    ATTGGAATGACTGTCGGTGCATTATTAATTGGTATCATTTCTCAGCCGTTGGTAGCCTTT
    TACTTCACAACTATAACTATCATGTTAATAGTAATATTTCTTTATATAAAGATAGTTGAC
    TTTCAGCTAAAGTATGCCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1164

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02818
  • symbol: SAOUHSC_02818
  • description: hypothetical protein
  • length: 387
  • theoretical pI: 9.77147
  • theoretical MW: 43229.9
  • GRAVY: 1.11705

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 101.2)
    multidrug resistance protein (TIGR00880; HMM-score: 70.1)
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 55.6)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 55.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 28.7)
  • TheSEED:  
    Major facilitator family transporter 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 147.8)
    MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 20.3)
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 7)
    no clan definedDUF4220; Domain of unknown function (DUF4220) (PF13968; HMM-score: 5.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.147
    • Ymax_pos: 11
    • Cmax: 0.119
    • Cmax_pos: 34
    • Smax: 0.265
    • Smax_pos: 6
    • Smean: 0.214
    • D: 0.173
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWINATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRLFQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYIGIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFFTYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVTITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGVFNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]