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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2523 [new locus tag: SACOL_RS13215 ]
  • pan locus tag?: SAUPAN006148000
  • symbol: SACOL2523
  • pan gene symbol?:
  • synonym:
  • product: drug transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2523 [new locus tag: SACOL_RS13215 ]
  • symbol: SACOL2523
  • product: drug transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2584090..2585253
  • length: 1164
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGATTAATAGTAATATCAGATTATATATTCTAGGATTTTTAGCATTTTTCGCATCATTG
    ATTCAAAACATATACACGCCTATTATTCCGAGACTATATGACGATTTTCAAGTACCACTA
    TTTTGGATAAATGCGACAGTTGGCGGTTTTATATTTATCGTTGCAGTTATGCAAATTGTA
    CTTGGTAGAAGTATAGATTCTCGTGATTCGAAAAAAGTACTTTTAACGGGATTGGGAATA
    GTAATCATATCTAGCTTTATTTGTGCAGTGACACATAACTTTATATTATTTGCTATATCA
    AGGTTATTTCAAGCAATTGGTTGCGGAATTATTCCTCTCGTGACTTTAACTTTATTAGCC
    AAACTAAGTACAGATAATGGTAGAGCTCAAGCTATGGCGAACTATCAAATATTTTTATCA
    TGTGCACCAGCTTTGGCACCAATCTTAGGGAGTACTTTAGGTGCAAGATGGGATTATATT
    GGTATTTTTAGTTTTTTGCTCGTTATATCTATCGTATTATTTTTGATTATCTTTTTTATT
    GATATTCCAAATGTTGAAAAAGGGATTGTAAAATTAACTGAAAAAATTGAAGAAAAATAT
    TTGACCGATAAAGTGTTTATTACTTTAGTGACATTGGGATTTTTAATATTTTTTACGTAC
    TTTTCAATATTAGTTTATTTACCAACATTATTAAATAATACCTATGATATAGGCGTTGGA
    ATATCAGGAGTTTTGTTTTTACCTATTACTGTTAGTGTTATATTAGGAAGTTTATTTTAT
    AAGCGATTTTCAAAAAAATATAATGAAATAATGATACTTCGTGTTACTATAACTGGATTT
    GCTATATTTACGTTATTGTTTGGATGGTTAAACGAATCAAATGTAATTATATTATCAGTA
    ATTATATTTATATTAGGTACTTGTGTTGGTATTGTGCCAGCTTTGCTGTCTACTATAATT
    TCTAAAAGGTTTGAACATATAAAAGGGAAAGTGCTAGGTGTATTTAATTTTGTGAGATAT
    ATTGGAATGACTGTCGGTGCATTATTAATTGGTATCATTTCTCAGCCGTTGGTAGCCTTT
    TACTTCACAACTATAACTATCATGTTAATAGTAATATTTCTTTATATAAAGATAGTTGAC
    TTTCAGCTAAAGTATGCCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1164

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL2523 [new locus tag: SACOL_RS13215 ]
  • symbol: SACOL2523
  • description: drug transporter
  • length: 387
  • theoretical pI: 9.77147
  • theoretical MW: 43229.9
  • GRAVY: 1.11705

Function[edit | edit source]

  • TIGRFAM:
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 101.2)
    and 4 more
    multidrug resistance protein (TIGR00880; HMM-score: 70.1)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 55.6)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 55.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 28.7)
  • TheSEED  :
    • Major facilitator family transporter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 147.8)
    and 3 more
    MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 20.3)
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 7)
    no clan defined DUF4220; Domain of unknown function (DUF4220) (PF13968; HMM-score: 5.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027152
    • TAT(Tat/SPI): 0.000354
    • LIPO(Sec/SPII): 0.064259
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWINATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRLFQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYIGIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFFTYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVTITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGVFNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]