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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02879
  • pan locus tag?: SAUPAN006226000
  • symbol: SAOUHSC_02879
  • pan gene symbol?: crtM
  • synonym:
  • product: squalene desaturase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02879
  • symbol: SAOUHSC_02879
  • product: squalene desaturase
  • replicon: chromosome
  • strand: -
  • coordinates: 2652287..2653150
  • length: 864
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACAATGATGGATATGAATTTTAAATATTGTCATAAAATCATGAAGAAACATTCAAAA
    AGCTTTTCTTACGCTTTTGACTTGTTACCAGAAGATCAAAGAAAAGCGGTTTGGGCAATT
    TATGCTGTGTGTCGTAAAATTGATGACAGTATAGATGTTTATGGCGATATTCAATTTTTA
    AATCAAATAAAAGAAGATATACAATCTATTGAAAAATACCCATATGAACATCATCACTTT
    CAAAGTGATCGTAGAATCATGATGGCGCTTCAGCATGTTGCACAACATAAAAATATCGCC
    TTTCAATCTTTTTATAATCTCATTGATACTGTATATAAAGATCAACATTTTACAATGTTT
    GAAACGGACGCTGAATTATTCGGATATTGTTATGGTGTTGCTGGTACAGTAGGTGAAGTA
    TTGACGCCGATTTTAAGTGATCATGAAACACATCAGACATACGATGTCGCAAGAAGACTT
    GGTGAATCGTTGCAATTGATTAATATATTAAGAGATGTCGGTGAAGATTTTGACAATGAA
    CGGATATATTTTAGTAAGCAACGATTAAAGCAATATGAAGTTGATATTGCTGAAGTGTAC
    CAAAATGGTGTTAATAATCATTATATTGACTTATGGGAATATTATGCAGCTATCGCAGAA
    AAAGATTTTCAAGATGTTATGGATCAAATCAAAGTATTTAGTATTGAAGCACAACCAATC
    ATAGAATTAGCAGCACGTATATATATTGAAATACTGGACGAAGTGAGACAGGCTAACTAT
    ACATTACATGAACGTGTTTTTGTGGATAAGAGGAAAAAGGCAAAGTTGTTTCATGAAATA
    AATAGTAAATATCATAGAATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    864

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02879
  • symbol: SAOUHSC_02879
  • description: squalene desaturase
  • length: 287
  • theoretical pI: 6.01098
  • theoretical MW: 34230.7
  • GRAVY: -0.449477

Function[edit source | edit]

  • reaction:
    EC 2.5.1.96?  ExPASy
    4,4'-diapophytoene synthase2 (2E,6E)-farnesyl diphosphate = 15-cis-4,4'-diapophytoene + 2 diphosphate
  • TIGRFAM:
    squalene synthase HpnD (TIGR03465; EC 2.5.1.21; HMM-score: 144.9)
    squalene synthase HpnC (TIGR03464; EC 2.4.1.21; HMM-score: 101.2)
    farnesyl-diphosphate farnesyltransferase (TIGR01559; EC 2.5.1.21; HMM-score: 51.1)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsCarotenoids Dehydrosqualene synthase (EC 2.5.1.96) 
    IsoprenoidsPolyprenyl Diphosphate Biosynthesis Dehydrosqualene synthase (EC 2.5.1.96) 
    CBSS-196620.1.peg.2477 Dehydrosqualene synthase (EC 2.5.1.96) 
  • PFAM:
    Terp_synthase (CL0613) SQS_PSY; Squalene/phytoene synthase (PF00494; HMM-score: 195.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.235
    • Ymax_pos: 18
    • Cmax: 0.111
    • Cmax_pos: 40
    • Smax: 0.615
    • Smax_pos: 11
    • Smean: 0.498
    • D: 0.337
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVCRKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQSFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRLGESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAIAEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKLFHEINSKYHRI

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 (SigB*) [3] [4] other strains
    SigB* (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]

Daisuke Umeno, Alexander V Tobias, Frances H Arnold
Evolution of the C30 carotenoid synthase CrtM for function in a C40 pathway.
J. Bacteriol.: 2002, 184(23);6690-9
[PubMed:12426357] [WorldCat.org] (P p)
Daisuke Umeno, Frances H Arnold
A C35 carotenoid biosynthetic pathway.
Appl. Environ. Microbiol.: 2003, 69(6);3573-9
[PubMed:12788765] [WorldCat.org] (P p)
Daisuke Umeno, Kaori Hiraga, Frances H Arnold
Method to protect a targeted amino acid residue during random mutagenesis.
Nucleic Acids Res.: 2003, 31(16);e91
[PubMed:12907743] [WorldCat.org] (I p)
Daisuke Umeno, Frances H Arnold
Evolution of a pathway to novel long-chain carotenoids.
J. Bacteriol.: 2004, 186(5);1531-6
[PubMed:14973014] [WorldCat.org] (P p)
Bosung Ku, Jae-Cheol Jeong, Benjamin N Mijts, Claudia Schmidt-Dannert, Jonathan S Dordick
Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway.
Appl. Environ. Microbiol.: 2005, 71(11);6578-83
[PubMed:16269684] [WorldCat.org] [DOI] (P p)