NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2337 [new locus tag: SAUSA300_RS12905 ]
• pan locus tag^?: SAUPAN005922000
• symbol: SAUSA300_2337
• pan gene symbol^?: nreC
• synonym:
• product: DegU family transcriptional regulator

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2337 [new locus tag: SAUSA300_RS12905 ]
• symbol: SAUSA300_2337
• product: DegU family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 2511799..2512452
• length: 654
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913825 NCBI
• RefSeq: YP_494972 NCBI
• BioCyc: GH3C-2324 BioCyc
• MicrobesOnline: 1293852 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGAAAATAGTCATTGCCGATGATCACGCTGTTGTCCGTACGGGGTTCTCTATGATTTTA
  AATTATCAAAATGATATGGAAGTTGTTGCAACGGCTGCAGATGGCGTCGAAGCTTACCAA
  AAAGTAATGGAATATAAACCTGATGTGTTACTAATGGATTTAAGTATGCCACCAGGTGAG
  TCAGGTCTTATCGCTACGAGTAAAATTGCTGACAGTTTTCCTGAAACTAAAATACTAATA
  TTAACAATGTTTGATGATGAGGAGTATTTGTTCCATGTGTTGCGTAATGGTGCGAAAGGT
  TACATATTGAAAAATGCACCTGATGAACAATTATTGTTAGCTATTCGAACTGTATATAAA
  GGTGAAACATATGTAGATATGAAACTTACAACATCTTTAGTGAATGAATTTGTATCTAAT
  TCAAATCAGGACACTGCAAACACAACAGATCCTTTTAAAATCTTATCAAAACGAGAACTA
  GAAATATTGCCACTTATTGCCAAAGGTTACGGGAATAAAGAAATTGCAGAGAAATTATTT
  GTATCTGTGAAAACAGTAGAAGCACATAAGACGCATATTATGACAAAGCTTGGCTTAAAG
  AGTAAACCTGAGCTAGTTGAATATGCATTGAAAAAGAAATTATTAGAGTTTTAG
  60
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  654
  

⊟Protein

⊟General

• locus tag: SAUSA300_2337 [new locus tag: SAUSA300_RS12905 ]
• symbol: SAUSA300_2337
• description: DegU family transcriptional regulator
• length: 217
• theoretical pI: 5.39357
• theoretical MW: 24368.2
• GRAVY: -0.0778802

⊟Function

• TIGRFAM:
  Cellular processesCellular processesSporulation and germinationsporulation transcription factor Spo0A (TIGR02875; HMM-score: 55)
  and 13 more
  LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 39)
  transcriptional regulator EpsA (TIGR03020; HMM-score: 37.5)
  Signal transductionRegulatory functionsDNA interactionsphosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)
  Signal transductionSignal transductionTwo-component systemsphosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)
  MetabolismCentral intermediary metabolismNitrogen metabolismnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
  Signal transductionRegulatory functionsDNA interactionsnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
  Signal transductionSignal transductionTwo-component systemsnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
  Signal transductionRegulatory functionsDNA interactionsPEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 31.7)
  RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 24.5)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 23.5)
  Signal transductionRegulatory functionsDNA interactionsheavy metal response regulator (TIGR01387; HMM-score: 21.7)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 18.8)
  Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 11.2)
• TheSEED:  
  Two-component transcriptional response regulator, LuxR family 
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 88.1)
  HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 78.1)
  and 6 more
  Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 19.4)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 16.5)
  DUF2089; Protein of unknown function (DUF2089) (PF09862; HMM-score: 14.3)
  Sigma70_ECF; ECF sigma factor (PF07638; HMM-score: 14)
  Pilus (CL0327) Pilin_PilX; Minor type IV pilin, PilX (PF11530; HMM-score: 12.9)
  HTH (CL0123) HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 11.9)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effector: NreB (sensor histidine kinase) sensing nitrate and nitrite
• genes regulated by NreC*, TF important in Nitrate and nitrite respirationRegPrecise

  activation

  narK

  SAUSA300_2337 < SAUSA300_2338 < SAUSA300_2339 < narI < narJ < narH < SAUSA300_2343

  SAUSA300_2344 < nirD < nirB < nirR

  transcription units transferred from N315 data RegPrecise
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.204
  • Ymax_pos: 33
  • Cmax: 0.226
  • Cmax_pos: 33
  • Smax: 0.325
  • Smax_pos: 27
  • Smean: 0.13
  • D: 0.175
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162341 NCBI
• RefSeq: YP_494972 NCBI
• UniProt: Q2FEA6 UniProt

⊟Protein sequence

• MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKRELEILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2337 < SAUSA300_2338 < SAUSA300_2339 < narI < narJ < narH < SAUSA300_2343

⊟Regulation

• sigma factor:
• regulators: NreC* (activation) regulon, Rex (repression) regulon

  NreC	(TF)	important in Nitrate and nitrite respiration;	RegPrecise    transcription unit transferred from N315 data RegPrecise
  Rex	(TF)	important in Energy metabolism;	RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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