NCBI: 10-JUN-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2337 [new locus tag: SAUSA300_RS12905 ]
- pan locus tag?: SAUPAN005922000
- symbol: SAUSA300_2337
- pan gene symbol?: nreC
- synonym:
- product: DegU family transcriptional regulator
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SAUSA300_2337 [new locus tag: SAUSA300_RS12905 ]
- symbol: SAUSA300_2337
- product: DegU family transcriptional regulator
- replicon: chromosome
- strand: -
- coordinates: 2511799..2512452
- length: 654
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 3913825 NCBI
- RefSeq: YP_494972 NCBI
- MicrobesOnline: 1293852 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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601TTGAAAATAGTCATTGCCGATGATCACGCTGTTGTCCGTACGGGGTTCTCTATGATTTTA
AATTATCAAAATGATATGGAAGTTGTTGCAACGGCTGCAGATGGCGTCGAAGCTTACCAA
AAAGTAATGGAATATAAACCTGATGTGTTACTAATGGATTTAAGTATGCCACCAGGTGAG
TCAGGTCTTATCGCTACGAGTAAAATTGCTGACAGTTTTCCTGAAACTAAAATACTAATA
TTAACAATGTTTGATGATGAGGAGTATTTGTTCCATGTGTTGCGTAATGGTGCGAAAGGT
TACATATTGAAAAATGCACCTGATGAACAATTATTGTTAGCTATTCGAACTGTATATAAA
GGTGAAACATATGTAGATATGAAACTTACAACATCTTTAGTGAATGAATTTGTATCTAAT
TCAAATCAGGACACTGCAAACACAACAGATCCTTTTAAAATCTTATCAAAACGAGAACTA
GAAATATTGCCACTTATTGCCAAAGGTTACGGGAATAAAGAAATTGCAGAGAAATTATTT
GTATCTGTGAAAACAGTAGAAGCACATAAGACGCATATTATGACAAAGCTTGGCTTAAAG
AGTAAACCTGAGCTAGTTGAATATGCATTGAAAAAGAAATTATTAGAGTTTTAG60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SAUSA300_2337 [new locus tag: SAUSA300_RS12905 ]
- symbol: SAUSA300_2337
- description: DegU family transcriptional regulator
- length: 217
- theoretical pI: 5.39357
- theoretical MW: 24368.2
- GRAVY: -0.0778802
⊟Function[edit source | edit]
- reaction:
- TIGRFAM: Cellular processesSporulation and germinationsporulation transcription factor Spo0A (TIGR02875; HMM-score: 55)LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 39)transcriptional regulator EpsA (TIGR03020; HMM-score: 37.5)Regulatory functionsDNA interactionsphosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)Signal transductionTwo-component systemsphosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)Central intermediary metabolismNitrogen metabolismnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)Regulatory functionsDNA interactionsnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)Signal transductionTwo-component systemsnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)Regulatory functionsDNA interactionsPEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 31.7)RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 24.5)proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 23.5)Regulatory functionsDNA interactionsheavy metal response regulator (TIGR01387; HMM-score: 21.7)RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 18.8)Signal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 11.2)
- TheSEED:
- PFAM: CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 88.1)HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 78.1)Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 19.4)HTH_23; Homeodomain-like domain (PF13384; HMM-score: 16.5)DUF2089; Protein of unknown function (DUF2089) (PF09862; HMM-score: 14.3)Sigma70_ECF; ECF sigma factor (PF07638; HMM-score: 14)Pilus (CL0327) Pilin_PilX; Minor type IV pilin, PilX (PF11530; HMM-score: 12.9)HTH (CL0123) HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 11.9)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular Possibility: 1
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.204
- Ymax_pos: 33
- Cmax: 0.226
- Cmax_pos: 33
- Smax: 0.325
- Smax_pos: 27
- Smean: 0.13
- D: 0.175
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKRELEILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: SAUSA300_2337 < SAUSA300_2338 < SAUSA300_2339 < narI < narJ < narH < SAUSA300_2343
⊟Regulation[edit source | edit]
- sigma factor:
- regulators: SAUSA300_2337 (NreC*), Rex
NreC* (TF) important in Nitrate and nitrite respiration RegPrecise transcription unit transferred from N315 data RegPrecise Rex (TF) important in Energy metabolism RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]