NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14180 [old locus tag: SAUSA300_2552 ]
• pan locus tag^?: SAUPAN006323000
• symbol: SAUSA300_RS14180
• pan gene symbol^?: citM
• synonym:
• product: citrate transporter

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14180 [old locus tag: SAUSA300_2552 ]
• symbol: SAUSA300_RS14180
• product: citrate transporter
• replicon: chromosome
• strand: -
• coordinates: 2759482..2760852
• length: 1371
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (2759482..2760852) NCBI
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGAATAGTGATAATATGTGGTTAACAGTAATGGGGCTCATTATTATTATTTCAATTGTA
  GGTTTACTCATTGCCAAAAAGATAAATCCAGTTGTAGGTATGACAATCATACCTTGCTTA
  GGGGCAATGATTTTAGGATATAGTGTGACAGATTTGGTTGGATTTTTTGCTAAAGGGTTA
  GATCAAGTCATCAACGTTGTTATTATGTTTATCTTTGCCATTATTTTCTTTGGCATCATG
  AACGATAGTGGTTTATTCAAGCCGCTTGTCAAACGCTTAATATTAATGACACGAGGCAAT
  GTCGTCATTGTCTGTGCAATGACAGCTTTAATTGGCACAATAGCCCAATTAGATGGGGCC
  GGTGCGGTAACATTTTTGCTTTCTATTCCTGCATTATTACCTTTATATAAAGCGTTAAAT
  ATGAATAAATATTTATTGATTTTACTATTAGCATTAAGCGCGGCGATTATGAACATGGTA
  CCTTGGGGAGGTCCAATGGCTCGTGTAGCTGCAGTGTTAAAAGCCAAAAGTGTCAATGAA
  TTATGGTATGGATTAATACCTATTCAAATAATAGGTTTCATTCTTGTTATGTTGTTTGCG
  GTATATCTTGGATTTAAAGAACAGAAACGTATCAAAAAAGCAATAGAGAGAAATGAATTA
  CCGCAAACACAAGATATAGATGTACATAAATTAGTTGAAGTATATGAACGAGATCAAGAT
  GTAAGGTTTCCTGTAAAAGGACGTGCAAGAACAAAATCATGGATAAAATGGGTGAATACA
  GCTTTAACTTTAGCTGTTATTCTATCGATGTTAATAAATATTGCGCCACCTGAATTTGCA
  TTCATGATAGGTGTTTCGTTGGCACTTGTTATTAATTTTAAATCAGTGGATGAACAAATG
  GAACGATTAAGAGCGCATGCGCCGAATGCATTAATGATGGCTGCAGTGATTATTGCAGCA
  GGTATGTTTTTAGGTGTACTAAATGAAACCGGTATGCTTAAAGCGATTGCGACCAATTTA
  ATCAAAGTGATTCCTGCAGAAGTAGGACCATACTTGCATATTATTGTAGGTTTACTTGGC
  GTACCATTAGATTTACTAACTAGCACAGACGCTTATTATTTTGCTGTGTTACCGATTGTC
  GAACAAACAGCAGGGCAATTTGGTGTACCGTCTGTATCAACAGCTTATTCAATGGTCATA
  GGGAATATTATAGGTACATTTGTCAGCCCATTTTCACCAGCCTTATGGTTGGCAATTGGT
  TTAGCAGAGGCAAACATGGGCACGTATATTAAGTATGCATTCTTTTGGATTTGGGGATTC
  GCTATCGTTATGTTAGTAATTGCAATGTTGATGGGCATTGTGACGATTTAA
  60
  120
  180
  240
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  420
  480
  540
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  720
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  840
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  1020
  1080
  1140
  1200
  1260
  1320
  1371
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS14180 [old locus tag: SAUSA300_2552 ]
• symbol: SAUSA300_RS14180
• description: citrate transporter
• length: 456
• theoretical pI: 9.61164
• theoretical MW: 49771.1
• GRAVY: 0.952412

⊟Function

• reaction:
• TIGRFAM:
  MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidscitrate transporter (TIGR00784; HMM-score: 279.8)
  Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 13.3)
• TheSEED:
• PFAM:
  IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 131.5)
  DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 26.9)
  DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 23.4)
  Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 21.6)
  Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 18.3)
  no clan definedGram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 11.9)
  Caudo_bapla_RBP; Receptor-binding protein of phage tail base-plate Siphoviridae, head (PF08931; HMM-score: 11)
  LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 9.6)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.163
  • Ymax_pos: 28
  • Cmax: 0.213
  • Cmax_pos: 28
  • Smax: 0.186
  • Smax_pos: 25
  • Smean: 0.117
  • D: 0.145
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 447006243 NCBI
• RefSeq: WP_001083499 NCBI
• UniProt:

⊟Protein sequence

• MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMILGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVVIVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMVPWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERNELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAPPEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLKAIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSVSTAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAMLMGIVTI

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SAUSA300_RS14180 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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