PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1077 [new locus tag: NWMN_RS06105 ]
• pan locus tag^?: SAUPAN003428000
• symbol: NWMN_1077
• pan gene symbol^?: ssl14
• synonym:
• product: superantigen-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1077 [new locus tag: NWMN_RS06105 ]
• symbol: NWMN_1077
• product: superantigen-like protein
• replicon: chromosome
• strand: -
• coordinates: 1180425..1181150
• length: 726
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330663 NCBI
• RefSeq: YP_001332111 NCBI
• BioCyc:
• MicrobesOnline: 3706628 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAAAGAACATCATGAATAAATTAGTTTTATCAACAGCATTGTTACTTTTAGAAACT
  ACATCAACACAACTTCCTAAAACACCAATCAGTTTTTCATCTGAAGCAAAAGCCTATAAT
  ATCAGTGAAAACGAGACTAATATCAATGAACTAATCAAATATTACACTCAGCCGCATTTT
  TCATTATCTGGAAAATGGTTATGGCAAAAGCCCAATGGTAGCATTCATGCAACATTGCAA
  ACGTGGGTTTGGTATAGTCATATTCAAGTGTTTGGATCCGAGAGTTGGGGAAACATTAAT
  CAGTTAAGAAATAAATACGTTGATATATTTGGAACTAAAGATGAGGACACAGTTGAAGGT
  TACTGGACTTATGATGAAACATTTACTGGTGGTGTTACGCCAGCAGCTACTTCATCTGAT
  AAGCCTTATAGACTATTTTTAAAATATAGTGATAAACAACAAACTATCATCGGTGGACAT
  GAATTTTACAAAGGAAATAAACCAGTATTAACTTTAAAAGAATTAGATTTCCGTATTCGT
  CAAACATTAATAAAAAATAAAAAGTTATATAACGGAGAATTTAATAAAGGTCAAATTAAG
  ATAACTGCTGATGGAAATAATTACACGATTGATTTAAGTAAAAAGTTAAAATTAACTGAC
  ACAAACCGTTATGTTAAAAATCCTCGTAATGCAGAAATTGAAGTCATACTCGAAAAATCT
  AACTAA
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⊟Protein

⊟General

• locus tag: NWMN_1077 [new locus tag: NWMN_RS06105 ]
• symbol: NWMN_1077
• description: superantigen-like protein
• length: 241
• theoretical pI: 9.74236
• theoretical MW: 27794.3
• GRAVY: -0.641079

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 32.5)
  and 2 more
  no clan defined Dicty_CAD; Cell-cell adhesion domain (PF05720; HMM-score: 13.8)
  Lsr2; Lsr2 (PF11774; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.01
  • Extracellular Score: 9.98
  • Internal Helices: 0
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: PISFSSEA
• SignalP: Signal peptide SP(Sec/SPI) length 38 aa
  • SP(Sec/SPI): 0.877821
  • TAT(Tat/SPI): 0.005262
  • LIPO(Sec/SPII): 0.073188
  • Cleavage Site: CS pos: 38-39. AKA-YN. Pr: 0.3634
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221289 NCBI
• RefSeq: YP_001332111 NCBI
• UniProt: A0A0H3K895 UniProt

⊟Protein sequence

• MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISENETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQLRNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGHEFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKLTDTNRYVKNPRNAEIEVILEKSN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1075 < NWMN_1076 < NWMN_1077

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Ilse Jongerius, Jörg Köhl, Manoj K Pandey, Maartje Ruyken, Kok P M van Kessel, Jos A G van Strijp, Suzan H M Rooijakkers
Staphylococcal complement evasion by various convertase-blocking molecules.
J Exp Med: 2007, 204(10);2461-71
[PubMed:17893203] [WorldCat.org] [DOI] (P p)