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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05375 [old locus tag: NWMN_0958 ]
  • pan locus tag?: SAUPAN003316000
  • symbol: NWMN_RS05375
  • pan gene symbol?: —
  • synonym:
  • product: cell-wall-binding lipoprotein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS05375 [old locus tag: NWMN_0958 ]
  • symbol: NWMN_RS05375
  • product: cell-wall-binding lipoprotein
  • replicon: chromosome
  • strand: +
  • coordinates: 1064320..1064946
  • length: 627
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1064320..1064946) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0958

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAATTTGGAAAAACAATCGCAGTAGTATTAGCATCTAGTGTCTTGCTTGCAGGATGT
    ACTACGGATAAAAAAGAAATTAAGGCATATTTAAAGCAAGTGGATAAAATTAAAGATGAT
    GAAGAACCAATTAAAACTGTTGGTAAGAAAATTGCTGAATTAGATGAGAAAAAGAAAAAA
    TTAACTGAAGATGTCAATAGTAAAGATACAGCAGTTCGCGGTAAAGCAGTAAAGGATTTA
    ATTAAAAATGCCGATGATCGTCTAAAGGAATTTGAAAAAGAAGAAGACGCAATTAAGAAG
    TCTGAACAAGACTTTAAGAAAGCAAAAAGTCACGTTGATAACATTGATAATGATGTTAAA
    CGTAAAGAAGTAAAACAATTAGATGATGTATTAAAAGAAAAATATAAGTTACACAGTGAT
    TACGCGAAAGCATATAAAAAGGCTGTAAACTCAGAGAAAACATTATTTAAATATTTAAAT
    CAAAATGACGCGACACAACAAGGTGTTAACGAAAAATCAAAAGCAATAGAACAGAACTAT
    AAAAAGTTAAAAGAAGTATCAGATAAGTATACAAAAGTACTAAATAAGGTTGGTAAAGAA
    AAGCAAGACGTTGATCAATTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS05375 [old locus tag: NWMN_0958 ]
  • symbol: NWMN_RS05375
  • description: cell-wall-binding lipoprotein
  • length: 208
  • theoretical pI: 9.96393
  • theoretical MW: 23875.2
  • GRAVY: -1.06587

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 15.4)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 15.4)
    type VII secretion effector, TIGR04197 family (TIGR04197; HMM-score: 12.8)
    and 6 more
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 9)
    SH3 domain protein (TIGR04211; HMM-score: 8.1)
    two transmembrane protein (TIGR04527; HMM-score: 7.7)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions conjugative transfer region lipoprotein, TIGR03751 family (TIGR03751; HMM-score: 5.9)
    phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 5.2)
  • TheSEED: see NWMN_0958
  • PFAM:
    no clan defined YkyA; Putative cell-wall binding lipoprotein (PF10368; HMM-score: 116.6)
    and 19 more
    Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 22)
    TPR (CL0020) COG6_N; Conserved oligomeric complex COG6, N-terminal (PF06419; HMM-score: 16.7)
    no clan defined Med4; Vitamin-D-receptor interacting Mediator subunit 4 (PF10018; HMM-score: 14.1)
    APG6_N; Apg6 coiled-coil region (PF17675; HMM-score: 13.7)
    DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 12.4)
    BORCS7; BLOC-1-related complex sub-unit 7 (PF16088; HMM-score: 12.1)
    DmpA_ArgJ (CL0635) MoCoBD_2; Molybdopterin cofactor-binding domain (PF20256; HMM-score: 12)
    TerB (CL0414) TerB; Tellurite resistance protein TerB (PF05099; HMM-score: 11.2)
    no clan defined BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 11.1)
    DASH_Duo1; DASH complex subunit Duo1 (PF08651; HMM-score: 10.6)
    IL31; Interleukin 31 (PF15209; HMM-score: 10.4)
    ABC_tran_CTD; ABC transporter C-terminal domain (PF16326; HMM-score: 9.9)
    Golgi-transport (CL0145) BAR_3; BAR domain of APPL family (PF16746; HMM-score: 8.7)
    MazG (CL0231) Nuf2_DHR10-like; Nuf2, DHR10-like domain (PF18595; HMM-score: 8.4)
    no clan defined Spc7; Spc7 kinetochore protein (PF08317; HMM-score: 7.6)
    P-loop_NTPase (CL0023) AAA_13; AAA domain (PF13166; HMM-score: 7.2)
    no clan defined UPF0242; Uncharacterised protein family (UPF0242) N-terminus (PF06785; HMM-score: 7.1)
    Cyclin (CL0065) DUF3452; Domain of unknown function (DUF3452) (PF11934; HMM-score: 6.8)
    no clan defined V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 5.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.8972
    • Cell wall & surface Score: 0.0592
    • Extracellular Score: 0.0435
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.000447
    • TAT(Tat/SPI): 0.000065
    • LIPO(Sec/SPII): 0.999242
    • Cleavage Site: CS pos: 19-20. LAG-CT. Pr: 0.9999
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPIKTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSEQDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLNQNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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