PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS05375 [old locus tag: NWMN_0958 ]
• pan locus tag^?: SAUPAN003316000
• symbol: NWMN_RS05375
• pan gene symbol^?: —
• synonym:
• product: cell-wall-binding lipoprotein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS05375 [old locus tag: NWMN_0958 ]
• symbol: NWMN_RS05375
• product: cell-wall-binding lipoprotein
• replicon: chromosome
• strand: +
• coordinates: 1064320..1064946
• length: 627
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1064320..1064946) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0958

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTTGGAAAAACAATCGCAGTAGTATTAGCATCTAGTGTCTTGCTTGCAGGATGT
  ACTACGGATAAAAAAGAAATTAAGGCATATTTAAAGCAAGTGGATAAAATTAAAGATGAT
  GAAGAACCAATTAAAACTGTTGGTAAGAAAATTGCTGAATTAGATGAGAAAAAGAAAAAA
  TTAACTGAAGATGTCAATAGTAAAGATACAGCAGTTCGCGGTAAAGCAGTAAAGGATTTA
  ATTAAAAATGCCGATGATCGTCTAAAGGAATTTGAAAAAGAAGAAGACGCAATTAAGAAG
  TCTGAACAAGACTTTAAGAAAGCAAAAAGTCACGTTGATAACATTGATAATGATGTTAAA
  CGTAAAGAAGTAAAACAATTAGATGATGTATTAAAAGAAAAATATAAGTTACACAGTGAT
  TACGCGAAAGCATATAAAAAGGCTGTAAACTCAGAGAAAACATTATTTAAATATTTAAAT
  CAAAATGACGCGACACAACAAGGTGTTAACGAAAAATCAAAAGCAATAGAACAGAACTAT
  AAAAAGTTAAAAGAAGTATCAGATAAGTATACAAAAGTACTAAATAAGGTTGGTAAAGAA
  AAGCAAGACGTTGATCAATTTAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS05375 [old locus tag: NWMN_0958 ]
• symbol: NWMN_RS05375
• description: cell-wall-binding lipoprotein
• length: 208
• theoretical pI: 9.96393
• theoretical MW: 23875.2
• GRAVY: -1.06587

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 15.4)
  Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 15.4)
  type VII secretion effector, TIGR04197 family (TIGR04197; HMM-score: 12.8)
  and 6 more
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 9)
  SH3 domain protein (TIGR04211; HMM-score: 8.1)
  two transmembrane protein (TIGR04527; HMM-score: 7.7)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions conjugative transfer region lipoprotein, TIGR03751 family (TIGR03751; HMM-score: 5.9)
  phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 5.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined YkyA; Putative cell-wall binding lipoprotein (PF10368; HMM-score: 171)
  and 16 more
  Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 18.6)
  Med4; Vitamin-D-receptor interacting Mediator subunit 4 (PF10018; HMM-score: 14.1)
  BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 12.5)
  BCLiA (CL0551) APG6; Autophagy protein Apg6 (PF04111; HMM-score: 12.3)
  no clan defined BORCS7; BLOC-1-related complex sub-unit 7 (PF16088; HMM-score: 12.3)
  IL31; Interleukin 31 (PF15209; HMM-score: 11.7)
  TerB (CL0414) TerB; Tellurite resistance protein TerB (PF05099; HMM-score: 11.6)
  no clan defined EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 11.2)
  DASH_Duo1; DASH complex subunit Duo1 (PF08651; HMM-score: 10.4)
  DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 9.9)
  T3SSipB; Type III cell invasion protein SipB (PF16535; HMM-score: 9.8)
  CENP-H; Centromere protein H (CENP-H) (PF05837; HMM-score: 9)
  P-loop_NTPase (CL0023) AAA_13; AAA domain (PF13166; HMM-score: 8.7)
  Golgi-transport (CL0145) BAR_3; BAR domain of APPL family (PF16746; HMM-score: 7)
  no clan defined IncA; IncA protein (PF04156; HMM-score: 6.8)
  V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 5.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.000447
  • TAT(Tat/SPI): 0.000065
  • LIPO(Sec/SPII): 0.999242
  • Cleavage Site: CS pos: 19-20. LAG-CT. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446590993 NCBI
• RefSeq: WP_000668339 NCBI
• UniProt: see NWMN_0958

⊟Protein sequence

• MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPIKTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSEQDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLNQNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications