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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09550 [old locus tag: NWMN_1700 ]
  • pan locus tag?: SAUPAN004556000
  • symbol: NWMN_RS09550
  • pan gene symbol?: hsdM2
  • synonym:
  • product: type I restriction-modification system subunit M

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS09550 [old locus tag: NWMN_1700 ]
  • symbol: NWMN_RS09550
  • product: type I restriction-modification system subunit M
  • replicon: chromosome
  • strand: -
  • coordinates: 1897366..1899105
  • length: 1740
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1897366..1899105) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1700

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATTTTGAAAGCATTTGAAAGCTACAACATTTCTATAAAATTTTTCAATAACAATTGC
    GCCACTAAAACTCAAAATTTCCACCACCAACATCCAAATTATCAACATCGCAACATAACC
    AAATGTTATAATAAATCTATTACACAAAGAGATAAATTACTTATGCAAAGGCGGAGGAAT
    CACATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGG
    TCGATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTA
    GGCTTGATTTTCTATCGCTTCTTATCTGAAAAAGCCGAACAAGAATATGCAGATGCCTTG
    TCAGGTGAAGACATCACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGACTTA
    AAAGCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGTGCG
    ATGATTCGTGAAATTGAAACGCAAGATTTCGATATAGAACATCTAGCGACGGCGATTCGC
    AAAGTTGAAACATCTACATTAGGTGAAGAAAGTGAAAATGACTTTATCGGGCTGTTCAGC
    GATATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCTTTAATC
    TCTAAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGAT
    ATGTTAGGTGATGCATATGAATTCCTAATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAA
    GCAGGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGAC
    GGTAAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCACTGTTGTTA
    CGTGTTGGTAAAGAAACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACA
    TACAACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGATATC
    CGTAATGATGACACATTGGAAAACCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATT
    GCGAACCCACCGTATAGTGCGAAATGGACTGCAGATTCAAAGTTTGAAAATGACGAACGA
    TTCAGTGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACAC
    ATGGTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTA
    TTCCGAGGTGCTGCAGAAGGTGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTA
    GAAGCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATT
    TTAGTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAAT
    GATTTTGAAAAAGGAAAAAATCAAAATCATTTAAGCGATGCCCAAGTCGAACGTATTATA
    GACACATATAAGCGTAAGGAAACAATTGATAAATATAGCTACAGCGCGACACTACAAGAG
    ATTGCCGATAACGATTACAACCTAAATATACCGAGATATGTCGATACATTCGAAGAAGAA
    GCACCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCA
    GAAATTGAGCAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS09550 [old locus tag: NWMN_1700 ]
  • symbol: NWMN_RS09550
  • description: type I restriction-modification system subunit M
  • length: 579
  • theoretical pI: 4.77081
  • theoretical MW: 66947.7
  • GRAVY: -0.562522

Function[edit | edit source]

  • reaction:
    EC 2.1.1.72?  ExPASy
    Site-specific DNA-methyltransferase (adenine-specific) S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467.2)
    and 5 more
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.5)
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 36.4)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 15.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.2)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 13.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 372)
    and 7 more
    no clan defined HsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 75)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 24.2)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 23.7)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 15.7)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.2)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.1)
    N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 13.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.060796
    • TAT(Tat/SPI): 0.001007
    • LIPO(Sec/SPII): 0.013465
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]