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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1012 [new locus tag: SA_RS05760 ]
- pan locus tag?: SAUPAN003429000
- symbol: argF
- pan gene symbol?: argF
- synonym:
- product: ornithine carbamoyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1012 [new locus tag: SA_RS05760 ]
- symbol: argF
- product: ornithine carbamoyltransferase
- replicon: chromosome
- strand: +
- coordinates: 1145818..1146819
- length: 1002
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1123842 NCBI
- RefSeq: NP_374285 NCBI
- BioCyc: see SA_RS05760
- MicrobesOnline: 103311 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAAAAATTTACGAAACAGAAGTTTTTTAACTTTATTAGACTTTTCACGACAAGAGGTA
GAATTCTTATTAACACTCTCCGAGGATTTAAAACGTGCTAAATATATTGGCACTGAAAAG
CCTATGTTAAAAAATAAAAATATTGCACTGTTATTTGAAAAAGATTCTACAAGAACGCGA
TGTGCATTTGAAGTTGCAGCGCATGATCAAGGTGCAAATGTAACTTATTTAGGCCCAACT
GGATCACAAATGGGTAAAAAAGAAACAACTAAAGATACTGCACGTGTGCTTGGTGGAATG
TATGATGGCATTGAATACCGTGGTTTTTCACAAAGAACAGTAGAAACTTTAGCTGAATAT
TCAGGCGTACCAGTGTGGAATGGTTTAACTGATGAAGATCATCCTACTCAAGTTCTTGCT
GATTTCTTAACAGCAAAAGAAGTCTTAAAAAAAGATTATGCAGATATTAACTTTACATAT
GTTGGAGATGGTCGTAATAACGTTGCAAATGCATTAATGCAAGGTGCTGCCATTATGGGT
ATGAACTTCCATTTAGTTTGTCCAAAAGAATTAAATCCAACAGATGAATTATTAAATCGC
TGTAAAAATATTGCCGCTGAAAATGGTGGCAACATATTAATCACAGATGATATTGACCAA
GGTGTAAAAGGTTCGGATGTAATTTACACTGATGTTTGGGTATCAATGGGTGAACCTGAT
GAAGTATGGAAAGAACGACTTGAATTATTGAAACCATATCAAGTAAATAAAGAAATGATG
GATAAAACTGGTAATCCAAATGTTATTTTTGAGCATTGCTTACCATCTTTCCATAATGCT
GATACGAAAATTGGTCAACAAATTTTTGAAAAATATGGTATTCGAGAAATGGAAGTTACA
GATGAAGTATTCGAAAGTAAAGCTTCAGTTGTATTCCAAGAAGCTGAGAACAGAATGCAT
ACAATCAAAGCAGTCATGGTTGCTACATTGGGTGAATTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1012 [new locus tag: SA_RS05760 ]
- symbol: ArgF
- description: ornithine carbamoyltransferase
- length: 333
- theoretical pI: 4.81544
- theoretical MW: 37534.4
- GRAVY: -0.405405
⊟Function[edit | edit source]
- reaction: EC 2.1.3.3? ExPASyOrnithine carbamoyltransferase Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
- TIGRFAM: Amino acid biosynthesis Glutamate family ornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 408.5)and 3 moreEnergy metabolism Amino acids and amines putrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 268.4)Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis aspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 120.5)knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 65)
- TheSEED :
- Ornithine carbamoyltransferase (EC 2.1.3.3)
Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation Ornithine carbamoyltransferase (EC 2.1.3.3)and 2 more - PFAM: Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 193)and 1 moreno clan defined OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 152.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003144
- TAT(Tat/SPI): 0.001114
- LIPO(Sec/SPII): 0.000657
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)