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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1181 [new locus tag: SACOL_RS06040 ]
  • pan locus tag?: SAUPAN003429000
  • symbol: arcB1
  • pan gene symbol?: argF
  • synonym:
  • product: ornithine carbamoyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1181 [new locus tag: SACOL_RS06040 ]
  • symbol: arcB1
  • product: ornithine carbamoyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1185397..1186398
  • length: 1002
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAATTTACGAAACAGAAGTTTTTTAACTTTATTAGACTTTTCACGACAAGAGGTA
    GAATTCTTATTAACACTCTCCGAGGATTTAAAACGTGCTAAATATATTGGCACTGAAAAG
    CCTATGTTAAAAAATAAAAATATTGCACTGTTATTTGAAAAAGATTCTACAAGAACGCGA
    TGTGCATTTGAAGTTGCAGCGCATGATCAAGGTGCAAATGTAACTTATTTAGGCCCAACT
    GGATCACAAATGGGTAAAAAAGAAACAACTAAAGATACTGCACGTGTGCTTGGTGGAATG
    TATGATGGCATTGAATACCGTGGTTTTTCACAAAGAACAGTAGAAACTTTAGCTGAGTAT
    TCAGGCGTACCAGTGTGGAATGGTTTAACTGATGAAGATCATCCTACTCAAGTTCTTGCT
    GATTTCTTAACAGCAAAAGAAGTCTTAAAAAAAGATTATGCAGATATTAACTTTACATAT
    GTTGGAGATGGTCGTAATAACGTTGCAAATGCATTAATGCAAGGTGCTGCCATTATGGGT
    ATGAACTTCCATTTAGTTTGTCCAAAAGAATTAAATCCAACAGATGAATTATTAAATCGC
    TGTAAAAATATTGCCGCTGAAAATGGTGGCAACATATTAATCACAGATGATATTGACCAA
    GGTGTAAAAGGTTCGGATGTAATTTACACTGATGTTTGGGTATCAATGGGTGAACCTGAT
    GAAGTATGGAAAGAACGACTTGAATTATTGAAACCATATCAAGTAAATAAAGAAATAATG
    GATAAAACTGGTAATCCAAATGTTATTTTTGAGCATTGCTTACCATCTTTCCATAATGCT
    GATACGAAAATAGGTCAACAAATTTTTGAAAAATATGGTATTCGAGAAATGGAAGTTACA
    GATGAAGTATTCGAAAGTAAAGCTTCAGTTGTATTCCAAGAAGCTGAGAACAGAATGCAT
    ACAATCAAAGCAGTCATGGTTGCTACATTGGGTGAATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1181 [new locus tag: SACOL_RS06040 ]
  • symbol: ArcB1
  • description: ornithine carbamoyltransferase
  • length: 333
  • theoretical pI: 4.81544
  • theoretical MW: 37516.4
  • GRAVY: -0.397598

Function[edit | edit source]

  • reaction:
    EC 2.1.3.3?  ExPASy
    Ornithine carbamoyltransferase Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family ornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 407.6)
    and 3 more
    Metabolism Energy metabolism Amino acids and amines putrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 266.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis aspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 120.3)
    knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 63.9)
  • TheSEED:  
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Ornithine carbamoyltransferase (EC 2.1.3.3)
    and 2 more
    Arginine Biosynthesis extended  Ornithine carbamoyltransferase (EC 2.1.3.3)
    Arginine Deiminase Pathway  Ornithine carbamoyltransferase (EC 2.1.3.3)
  • PFAM:
    Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 191.3)
    and 1 more
    no clan defined OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 152.7)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003144
    • TAT(Tat/SPI): 0.001114
    • LIPO(Sec/SPII): 0.000657
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]