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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0368 [new locus tag: SACOL_RS01850 ]
- pan locus tag?: SAUPAN001833000
- symbol: SACOL0368
- pan gene symbol?: —
- synonym:
- product: prophage L54a, HK97 family portal protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0368 [new locus tag: SACOL_RS01850 ]
- symbol: SACOL0368
- product: prophage L54a, HK97 family portal protein
- replicon: chromosome
- strand: +
- coordinates: 375923..377161
- length: 1239
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236797 NCBI
- RefSeq: YP_185260 NCBI
- BioCyc: see SACOL_RS01850
- MicrobesOnline: 911839 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201GTGAATGTTATCGCAAAAGAGAATATTGTCACACGCATAAAGAAAAAATTGATAGACAAT
TGGATTGATCAGTCAACTTCTAAGCTTTATGACTTTAGCCCATGGAAAAATAGATCTTTT
TGGGGTGTAATTAATAATACGCTTGAAACTAATGAAACGATATTTTCAGCTATTACAAAG
TTATCTAATTCGATGGCTAGTTTGCCCTTGAAAATGTATGAAGATTATAAAGTAGTTAAT
ACAGAAGTATCTGATTTACTTACAGTGTCACCGAATAATTCTCTGAGCAGTTTTGATTTT
ATTAATCAAATTGAAACAATCAGAAATGAAAAAGGTAATGCATATGTGCTAATTGAACGA
GACATCTATCATCAACCATCAAAGCTTTTCTTATTAAATCCAGATGTTGTTGAAATGTTA
ATTGAAAACCAATCACGTGAACTTTATTATTCCATTCATGCTGCAACTGGAAATAAATTG
ATTGTTCATAATATGGACATGTTGCATTTTAAACACATCGTGGCATCTAATATGGTGCAA
GGCATTAGTCCGATTGATGTGTTGAAGAATACAACTGATTTTGATAATGCAGTAAGAACC
TTTAATCTTACAGAAATGCAAAAACCTGATTCTTTCATGCTTAAATATGGTTCCAATGTA
GGTAAAGAAAAAAGGCAGCAAGTGTTAGAAGATTTCAAACAGTACTATGAAGAAAACGGT
GGAATATTATTCCAAGAGCCTGGTGTTGAAATCGAACCGTTACCTAAAAAATATGTCTCT
GAAGATATAGTGGCAAGCGAGAATTTAACAAGAGAAAGAGTAGCTAACGTTTTTCAATTG
CCCTCAGTATTCTTAAATGCAAGATCAAATACAAATTTCGCGAAAAATGAAGAGTTAAAC
AGATTTTACTTGCAGCATACCTTATTGCCAATCGTCAAACAGTATGAAGAAGAATTTAAT
CGGAAACTACTTACTAAAACAGACAGAGAAAAAAATAGGTATTTTAAATTTAACGTTAAA
TCTTATTTAAGGGCTGATAGTGCAACACAAGCAGAAGTGTACTTTAAAGCAGTTCGTAGT
GGTTACTACACTATAAATGACATTAGAGAGTGGGAGGATTTACCACCAGTTGAAGGTGGA
GATAAGCCGCTAATAAGCGGTGATTTATACCCAATTGACACGCCACTTGAATTAAGAAAA
TCTTTGAAAGGTGGTGATAAAAATGTCAATGAAAGCTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0368 [new locus tag: SACOL_RS01850 ]
- symbol: SACOL0368
- description: prophage L54a, HK97 family portal protein
- length: 412
- theoretical pI: 5.78936
- theoretical MW: 47727.8
- GRAVY: -0.520388
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006525
- TAT(Tat/SPI): 0.000125
- LIPO(Sec/SPII): 0.000427
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNVIAKENIVTRIKKKLIDNWIDQSTSKLYDFSPWKNRSFWGVINNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFINQIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKLIVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGSNVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVANVFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRYFKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPIDTPLELRKSLKGGDKNVNES
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p)