From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0369 [new locus tag: SACOL_RS01855 ]
  • pan locus tag?: SAUPAN001832000
  • symbol: SACOL0369
  • pan gene symbol?: —
  • synonym:
  • product: prophage L54a, Clp protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0369 [new locus tag: SACOL_RS01855 ]
  • symbol: SACOL0369
  • product: prophage L54a, Clp protease
  • replicon: chromosome
  • strand: +
  • coordinates: 377145..377918
  • length: 774
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCAATGAAAGCTAAGTATTTTCAAATGAAAAGAAAATCAAAAAGTAAAGGTGAAATA
    TTTATTTATGGTGATATTGTAAGTGATAAATGGTTTGAAAGTGATGTAACTGCTACAGAT
    TTCAAAAATAAACTAGATGAACTAGGAGACATCAGTGAAATAGATGTTCATATAAATTCA
    TCTGGAGGCAGTGTATTTGAAGGGCATGCAATATACAATATGCTAAAAATGCATCCTGCA
    AAAATTAATATCTATGTCGATGCTTTAGCGGCATCAATTGCTAGTGTTATCGCTATGAGT
    GGTGACACTATTTTTATGCACAAAAATAGTTTTTTAATGATTCATAATTCATGGGTTATG
    ACTGTAGGTAATGCAGAAGAATTAAGAAAGACAGCGGATTTACTTGAAAAAACAGATGCT
    GTTAGTAATTCAGCTTATTTAGATAAAGCAAAAGATTTAGATCAAGAACACTTAAAACAG
    ATGTTAGATGCAGAAACTTGGCTTACTGCAGAAGAAGCCTTGTCTTTCGGCTTGATAGAT
    GAAATTTTAGGAGCTAATGAAATAGCTGCTAGTATCTCTAAAGAGCAATATAAGCGTTTC
    GAGAACGTCCCAGAAGATTTAAAGAAAGATGTAGACAAAATCACTAAAATTGATGATGTA
    GATACATCTGAATTGGTTGAAACACCTAAAGAAAGTATGTCACTAGAAGAAAAAGAAAAA
    AGAGAAAAAATTAAACGCGAATGCGAAATTTTAAAAATGACAATGAATTATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    774

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0369 [new locus tag: SACOL_RS01855 ]
  • symbol: SACOL0369
  • description: prophage L54a, Clp protease
  • length: 257
  • theoretical pI: 4.71323
  • theoretical MW: 29170.1
  • GRAVY: -0.42179

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp endopeptidase, proteolytic subunit ClpP (TIGR00493; EC 3.4.21.92; HMM-score: 51.8)
    and 1 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides signal peptide peptidase SppA, 36K type (TIGR00706; EC 3.4.-.-; HMM-score: 35.8)
  • TheSEED  :
    • Phage head, head-tail preconnector protease C
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory cAMP signaling in bacteria  Prophage Clp protease-like protein
  • PFAM:
    ClpP_crotonase (CL0127) CLP_protease; Clp protease (PF00574; HMM-score: 105.7)
    and 2 more
    SDH_sah; Serine dehydrogenase proteinase (PF01972; HMM-score: 26.3)
    Peptidase_S49; Peptidase family S49 (PF01343; HMM-score: 17.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005815
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.000638
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNKLDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGDTIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQMLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKIDDVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL0369&oldid=90472"