From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01536
  • pan locus tag?: SAUPAN001832000
  • symbol: SAOUHSC_01536
  • pan gene symbol?: —
  • synonym:
  • product: scaffolding protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01536
  • symbol: SAOUHSC_01536
  • product: scaffolding protease
  • replicon: chromosome
  • strand: -
  • coordinates: 1484012..1484785
  • length: 774
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCAATGAAAGCTAAGTATTTTCAAATGAAAAGAAAATCAAAAAGTAAAGGTGAAATA
    TTCATTTATGGTGATATTGTAAGTGATAAATGGTTTGAAAGTGATGTAACTGCTACAGAT
    TTCAAAAATAAACTAGATGAACTAGGAGACATCAGTGAAATAGATGTTCATATAAATTCA
    TCTGGAGGCAGTGTATTTGAAGGACATGCAATATACAATATGCTAAAAATGCATCCTGCA
    AAAATTAATATCTATGTCGATGCCTTAGCGGCATCAATTGCTAGTGTTATCGCTATGAGT
    GGTGACACTATTTTTATGCACAAAAATAGTTTTTTAATGATTCATAATTCATGGGTTATG
    ACTGTAGGTAATGCAGAAGAATTAAGAAAGACAGCGGATTTACTTGAAAAAACAGATGCT
    GTTAGTAATTCAGCTTATTTAGATAAAGCAAAAGATTTAGATCAAGAACACTTAAAACAG
    ATGTTAGATGCAGAAACTTGGCTTACTGCAGAAGAAGCCTTGTCTTTCGGCTTGATAGAT
    GAAATTTTAGGAGCTAATGAAATAGCTGCTAGTATCTCTAAAGAGCAATATAAGCGTTTC
    GAGAACGTCCCAGAAGATTTAAAGAAAGATGTAGACAAAATCACTAAAATTGATGATGTA
    GATACATCTGAATTGGTTGAAACACCTAAAGAAAGTATGTCACTAGAAGAAAAAGAAAAA
    AGAGAAAAAATTAAACGCGAATGCGAAATTTTAAAAATGACAATGAATTATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    774

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01536
  • symbol: SAOUHSC_01536
  • description: scaffolding protease
  • length: 257
  • theoretical pI: 4.71323
  • theoretical MW: 29170.1
  • GRAVY: -0.42179

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp endopeptidase, proteolytic subunit ClpP (TIGR00493; EC 3.4.21.92; HMM-score: 51.8)
    and 1 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides signal peptide peptidase SppA, 36K type (TIGR00706; EC 3.4.-.-; HMM-score: 35.8)
  • TheSEED  :
    • Phage head, head-tail preconnector protease C
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory cAMP signaling in bacteria  Prophage Clp protease-like protein
  • PFAM:
    ClpP_crotonase (CL0127) CLP_protease; Clp protease (PF00574; HMM-score: 105.7)
    and 2 more
    SDH_sah; Serine dehydrogenase proteinase (PF01972; HMM-score: 26.3)
    Peptidase_S49; Peptidase family S49 (PF01343; HMM-score: 17.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005815
    • TAT(Tat/SPI): 0.000242
    • LIPO(Sec/SPII): 0.000638
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNKLDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGDTIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQMLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKIDDVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_01536&oldid=71693"