PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS10655 [old locus tag: SACOL2041 ]
• pan locus tag^?: SAUPAN005301000
• symbol: SACOL_RS10655
• pan gene symbol^?: tsaE
• synonym:
• product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS10655 [old locus tag: SACOL2041 ]
• symbol: SACOL_RS10655
• product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE
• replicon: chromosome
• strand: -
• coordinates: 2100097..2100531
• length: 435
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2100097..2100531) NCBI
• BioCyc: SACOL_RS10655 BioCyc
• MicrobesOnline: see SACOL2041

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  301
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  421
  
  ATGAATCAATTTGCTATATTTTTAGTTGAGCAATTGAAAAGTGGTGATTTGATTTTACTT
  AACGGAGATTTAGGAGCAGGTAAAACAACGTTAACGCAATTTATAGGAAAAGCTCTTGGT
  GTAAGACGTACGATTAATTCCCCGACATTTAACATCATTAAATCATATAGGGGTAAAAAT
  TTAAAATTGCATCATATGGATTGTTATCGCTTAGAAGATTCTGATGAAGATTTAGGATTT
  GATGAATTTTTCGAAGATCAGGCAATTACTGTTATTGAATGGAGTCAATTTATAAAAGAT
  TTACTTCCAGCGACGCATTTATCTATTAACATTTCAACAATATCTGAAAATACAAGACAA
  ATTGAGTTGTTCGCGCAAGGAGAACATTATGAACAAATTAAGGAGGCAATTATCCATGAA
  TTCGCTGCTCATTGA
  60
  120
  180
  240
  300
  360
  420
  435
  

⊟Protein

⊟General

• locus tag: SACOL_RS10655 [old locus tag: SACOL2041 ]
• symbol: SACOL_RS10655
• description: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE
• length: 144
• theoretical pI: 4.97011
• theoretical MW: 16441.5
• GRAVY: -0.189583

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 125.6)
  and 9 more
  ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 15.4)
  Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 14.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 12.7)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.4)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.4)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 12.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12)
  Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12)
  Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 10.7)
• TheSEED: see SACOL2041
• PFAM:
  P-loop_NTPase (CL0023) TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 119.5)
  and 12 more
  AAA_18; AAA domain (PF13238; HMM-score: 21.9)
  NACHT; NACHT domain (PF05729; HMM-score: 16.5)
  RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 14.9)
  AAA_23; AAA domain (PF13476; HMM-score: 14.9)
  dNK; Deoxynucleoside kinase (PF01712; HMM-score: 14.8)
  ABC_tran; ABC transporter (PF00005; HMM-score: 13.4)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13)
  AAA_22; AAA domain (PF13401; HMM-score: 12.7)
  AAA_33; AAA domain (PF13671; HMM-score: 12.7)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 12.5)
  TIM_barrel (CL0036) DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 12.2)
  P-loop_NTPase (CL0023) Zeta_toxin; Zeta toxin (PF06414; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010491
  • TAT(Tat/SPI): 0.001918
  • LIPO(Sec/SPII): 0.002381
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SACOL2041
• RefSeq: WP_010956572 NCBI
• UniProt: see SACOL2041

⊟Protein sequence

• MNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFIGKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIEWSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH

⊟Experimental data

• experimentally validated: see SACOL2041
• protein localization: see SACOL2041
• quantitative data / protein copy number per cell: see SACOL2041
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications