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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01085
  • pan locus tag?: SAUPAN003363000
  • symbol: SAOUHSC_01085
  • pan gene symbol?: isdE
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01085
  • symbol: SAOUHSC_01085
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1047415..1048263
  • length: 849
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGATAAGCGTCGTTATCTTAACCAGCTGTCAATCTTCCAGTTCTCAAGAATCAACTAAA
    TCCGGCGAATTCAGAATCGTACCAACAACTGTTGCATTGACAATGACATTGGACAAATTG
    GATTTACCAATTGTCGGCAAACCCACGTCATATAAGACATTGCCTAATCGTTATAAAGAT
    GTACCGGAAATTGGTCAACCAATGGAGCCGAATGTTGAAGCTGTTAAAAAGTTAAAACCA
    ACACATGTTTTGAGTGTGTCAACGATTAAAGATGAAATGCAACCATTTTACAAACAATTA
    AATATGAAAGGCTACTTTTATGATTTTGATAGTTTAAAAGGGATGCAAAAGTCGATTACA
    CAATTAGGTGATCAATTTAATCGTAAAGCACAAGCAAAAGAATTAAATGACCATTTAAAT
    TCTGTAAAGCAAAAAATTGAAAATAAAGCAGCTAAACAAAAGAAACATCCCAAAGTATTA
    ATATTAATGGGTGTACCGGGTAGCTATTTAGTAGCAACTGATAAATCATATATTGGTGAT
    TTAGTTAAAATAGCAGGTGGAGAAAATGTTATTAAAGTGAAAGATCGTCAATATATTTCG
    TCTAATACTGAAAATTTGTTGAATATCAATCCAGATATTATTTTACGATTACCACACGGA
    ATGCCTGAAGAAGTTAAGAAAATGTTTCAAAAAGAATTTAAACAAAATGATATTTGGAAA
    CATTTTAAAGCTGTGAAAAATAATCATGTTTATGACTTAGAGGAAGTGCCATTCGGTATT
    ACAGCAAATGTTGATGCTGATAAGGCAATGACTCAATTATATGATTTATTTTATAAGGAT
    AAAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    849

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01085
  • symbol: SAOUHSC_01085
  • description: hypothetical protein
  • length: 282
  • theoretical pI: 9.80489
  • theoretical MW: 32056.9
  • GRAVY: -0.50922

⊟Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 405.6)
    and 2 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 43.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase TatB (TIGR01410; HMM-score: 15.1)
  • TheSEED  :
    • Heme transporter IsdDEF, lipoprotein IsdE
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, lipoprotein IsdE
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 138.8)
    and 4 more
    no clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 17.2)
    HAD (CL0137) NT5C; 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (PF06941; HMM-score: 16.1)
    no clan defined DUF2098; Uncharacterized protein conserved in archaea (DUF2098) (PF09871; HMM-score: 15)
    PK_TIM (CL0151) ICL; Isocitrate lyase family (PF00463; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: heme b
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.076423
    • TAT(Tat/SPI): 0.007373
    • LIPO(Sec/SPII): 0.157625
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISVVILTSCQSSSSQESTKSGEFRIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

John M Taylor, David E Heinrichs
Transferrin binding in Staphylococcus aureus: involvement of a cell wall-anchored protein.
Mol Microbiol: 2002, 43(6);1603-14
[PubMed:11952908] [WorldCat.org] [DOI] (P p)John Mack, Christie Vermeiren, David E Heinrichs, Martin J Stillman
In vivo heme scavenging by Staphylococcus aureus IsdC and IsdE proteins.
Biochem Biophys Res Commun: 2004, 320(3);781-8
[PubMed:15240116] [WorldCat.org] [DOI] (P p)Mark Pluym, Christie L Vermeiren, John Mack, David E Heinrichs, Martin J Stillman
Heme binding properties of Staphylococcus aureus IsdE.
Biochemistry: 2007, 46(44);12777-87
[PubMed:17929943] [WorldCat.org] [DOI] (P p)

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