From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1032 [new locus tag: SAUSA300_RS05555 ]
  • pan locus tag?: SAUPAN003363000
  • symbol: SAUSA300_1032
  • pan gene symbol?: isdE
  • synonym:
  • product: putative iron compound ABC transporter iron compound-binding protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1032 [new locus tag: SAUSA300_RS05555 ]
  • symbol: SAUSA300_1032
  • product: putative iron compound ABC transporter iron compound-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1127538..1128386
  • length: 849
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGATAAGCGTCGTTATCTTAACCAGCTGTCAATCTTCCAGTTCTCAAGAATCAACTAAA
    TCCGGCGAATTCAGAATCGTACCAACAACTGTTGCATTGACAATGACATTGGACAAATTG
    GATTTACCAATTGTCGGCAAACCCACGTCATATAAGACATTGCCTAATCGTTATAAAGAT
    GTACCGGAAATTGGTCAACCAATGGAGCCGAATGTTGAAGCTGTTAAAAAGTTAAAACCA
    ACACATGTTTTGAGTGTGTCAACGATTAAAGATGAAATGCAACCATTTTACAAACAATTA
    AATATGAAAGGCTACTTTTATGATTTTGATAGTTTAAAAGGGATGCAAAAGTCGATTACA
    CAATTAGGTGATCAATTTAATCGTAAAGCACAAGCAAAAGAATTAAATGACCATTTAAAT
    TCTGTAAAGCAAAAAATTGAAAATAAAGCAGCTAAACAAAAGAAACATCCCAAAGTATTA
    ATATTAATGGGTGTACCGGGTAGCTATTTAGTAGCAACTGATAAATCATATATTGGTGAT
    TTAGTTAAAATAGCAGGTGGAGAAAATGTTATTAAAGTGAAAGATCGTCAATATATTTCG
    TCTAATACTGAAAATTTGTTGAATATCAATCCAGATATTATTTTACGATTACCACACGGA
    ATGCCTGAAGAAGTTAAGAAAATGTTTCAAAAAGAATTTAAACAAAATGATATTTGGAAA
    CATTTTAAAGCTGTGAAAAATAATCATGTTTATGACTTAGAGGAAGTGCCATTCGGTATT
    ACAGCAAATGTTGATGCTGATAAGGCAATGACTCAATTATATGATTTATTTTATAAGGAT
    AAAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    849

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1032 [new locus tag: SAUSA300_RS05555 ]
  • symbol: SAUSA300_1032
  • description: putative iron compound ABC transporter iron compound-binding protein
  • length: 282
  • theoretical pI: 9.80489
  • theoretical MW: 32056.9
  • GRAVY: -0.50922

Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 405.6)
    and 2 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 43.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase TatB (TIGR01410; HMM-score: 15.1)
  • TheSEED  :
    • Heme transporter IsdDEF, lipoprotein IsdE
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, lipoprotein IsdE
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 138.8)
    and 4 more
    no clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 17.2)
    HAD (CL0137) NT5C; 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (PF06941; HMM-score: 16.1)
    no clan defined DUF2098; Uncharacterized protein conserved in archaea (DUF2098) (PF09871; HMM-score: 15)
    PK_TIM (CL0151) ICL; Isocitrate lyase family (PF00463; HMM-score: 13.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: heme b
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.076423
    • TAT(Tat/SPI): 0.007373
    • LIPO(Sec/SPII): 0.157625
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MISVVILTSCQSSSSQESTKSGEFRIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDKK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]