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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02641
  • pan locus tag?: SAUPAN005869000
  • symbol: SAOUHSC_02641
  • pan gene symbol?: hrtB
  • synonym:
  • product: permease domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02641
  • symbol: SAOUHSC_02641
  • product: permease domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2426796..2427851
  • length: 1056
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAATTAGCGATAAAAGAGATTATGTTTTACAAATTTCGTTATATTTTAATCACATTA
    ATCATTCTTTTATTAAGTATTATGGTGTTATTTATTAGTGGTTTAGCTCAAGGGCTTGGT
    AGGGAGAATATTTCGTTATTTGAACACTTTGATAATGATGAATATGTTGTTCAAAAAATG
    AAAGAGCCGCAAATTGAGAAATCGCAACTTAGTGATACGCAACAGAATCAAATTAAAAAG
    GTAATTCATCAAGAACCATATAAAATGAATATTCAAACATTGAAATTATCTAATAAAGAA
    CAAGATGTGATTACAATGAATGACGTTAAACAACAACGCATACAACTTAAAAAAGGTGAT
    TATCCTAAAAATGCTCATGAGGTCGCTATTAATGATAAGTTAGCTGCAGACAACATTAGA
    GTCGGGGATAGATTACATTTTAAAAATAATTCAACTAGTTATAGAGTTTCTGGTATTTTA
    AACGACACAATGTATGCGCATAGTTCCATTGTGCTATTGAACGATAACGGATTTAATGCA
    TTGAATAAGGTTAATACGGCATTTTATCCAGTGAAAAATTTAACACAACAACAACGTGAT
    GAGCTTAATAAAATAAATGACGTTCAAGTTGTGAGTGAAAAAGATTTAACAGGTAATATT
    GCGAGTTATCAAGCAGAGCAAGCACCGTTAAATATGATGATTGTTAGTTTGTTTGCTATT
    ACAGCAATCGTTCTAAGTGCATTTTTCTATGTTATGACGATTCAAAAAATATCACAAATT
    GGCATTTTGAAAGCAATTGGTATTAAGACAAGACATTTATTGAGTGCGTTAGTTTTACAA
    ATTTTAACACTAACAATAATTGGGGTAGGTATTGCTGTGATCATCATAGTAGGACTATCA
    TTTATGATGCCGGTAACGATGCCTTTTTACTTAACAACGCAAAATATTTTATTAATGGTG
    GGGATATTTATATTAGTAGCGATTTTAGGTGCCTCACTATCATTTATCAAATTATTTAAA
    GTGGATCCTATCGAAGCAATTGGAGGTGCAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02641
  • symbol: SAOUHSC_02641
  • description: permease domain-containing protein
  • length: 351
  • theoretical pI: 9.58764
  • theoretical MW: 39481.3
  • GRAVY: 0.265242

⊟Function[edit | edit source]

  • TIGRFAM:
    acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 32.9)
    and 1 more
    Metabolism Transport and binding proteins Other ABC exporter transmembrane subunit, DevC protein (TIGR01185; HMM-score: 17.2)
  • TheSEED  :
    • Heme efflux system permease HrtB
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme efflux system permease HrtB
  • PFAM:
    no clan defined MacB_PCD; MacB-like periplasmic core domain (PF12704; HMM-score: 33.2)
    BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 26.8)
    and 1 more
    no clan defined DUF1195; Protein of unknown function (DUF1195) (PF06708; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.220691
    • TAT(Tat/SPI): 0.001317
    • LIPO(Sec/SPII): 0.060633
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENISLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQDVITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGILNDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTGNIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSALVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLSFIKLFKVDPIEAIGGAE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HssR* (activation) regulon
    HssR*(TF)important in Heme efflux; compare RegPrecise for N315  [3]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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