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Summary[edit | edit source]

  • pan ID?: SAUPAN005722000
  • symbol?:
  • synonym:
  • description?: MarR family transcriptional regulator

      descriptions from strain specific annotations:

    • MarR family transcriptional regulator
    • MarR family regulatory protein
    • transcriptional regulator, putative
    • winged helix DNA-binding protein
    • HTH-type transcriptional regulator
    • marR family protein
    • transcriptional regulator, MarR family
  • strand?: +
  • coordinates?: 5827475..5827915
  • synteny block?: BlockID0044680
  • occurrence?: in 100% of 34 strains

mepR2 : multidrug efflux pump 2 transcriptional repressor MepR2 [1]

• A crystal structure is available : 4EM1

There is some confusion in the S. aureus literature regarding the MepRA transporters. Multiple papers and two crystal structures are available referring to two separate operons as MepRA. For clarity, we will annotate the operon between the ascorbate phosphotransferase operon and the glycerol-3-phosphate transporter (3ECO) as "Mep1" and the one between femX and sarV (4EM1) as Mep2.

Orthologs[edit | edit source]

    COL:
    N315:
    NCTC8325:
    Newman:
    USA300_FPR3757:
    JSNZ:
    04-02981:
    SA2981_2203
    08BA02176:
    C248_2302
    11819-97:
    MS7_2284
    6850:
    RSAU_002099
    71193:
    ST398NM01_2316
    ECT-R 2:
    ECTR2_2126
    ED133:
    SAOV_2303
    ED98:
    SAAV_2328
    HO 5096 0412:
    SAEMRSA15_21630
    JH1:
    SaurJH1_2332
    JH9:
    SaurJH9_2290
    JKD6008:
    SAA6008_02302
    JKD6159:
    SAA6159_02170
    LGA251:
    SARLGA251_20490
    M013:
    M013TW_2223
    MRSA252:
    SAR2349
    MSHR1132:
    SAMSHR1132_20900
    MSSA476:
    SAS2155
    Mu3:
    SAHV_2249
    Mu50:
    SAV2265
    MW2:
    MW2183
    RF122:
    SAB2137
    ST398:
    SAPIG2316
    T0131:
    SAT0131_02440
    TCH60:
    HMPREF0772_10935
    TW20:
    SATW20_23970
    USA300_TCH1516:
    USA300HOU_2246 (marR)
    VC40:
    SAVC_10185

Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757
JSNZ

Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.505)


    COL             MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKHQPTTLIEISHRRAIEK
    N315            MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKHQPTTLIEISHRRAIEK
    NCTC8325        MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKHQPTTLIEISHRRAIEK
    Newman          MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKHQPTTLIEISHRRAIEK
    USA300_FPR3757  MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKHQPTTLIEISHRRAIEK
    JSNZ            MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKHQPTTLIEISHRRAIEK
                    ************************************************************

    COL             PTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEIVCLDVQKLQQAVVAKTNI
    N315            PTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEIVCLDVQKLQQAVVAKTNI
    NCTC8325        PTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEIVCLDVQKLQQAVVAKTNI
    Newman          PTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEIVCLDVQKLQQAVVAKTNI
    USA300_FPR3757  PTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEIVCLDVQKLQQAVVAKTNI
    JSNZ            PTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEIVCLDVQKLQQAVVAKTNI
                    ************************************************************

    COL             SQDQMQETINVMNQIHEILLKEAHND
    N315            SQDQMQETINVMNQIHEILLKEAHND
    NCTC8325        SQDQMQETINVMNQIHEILLKEAHND
    Newman          SQDQMQETINVMNQIHEILLKEAHND
    USA300_FPR3757  SQDQMQETINVMNQIHEILLREAHND
    JSNZ            SQDQMQETINVMNQIHEILLKEAHND
                    ********************:*****

  1. Muthiah Kumaraswami, Jason T Schuman, Susan M Seo, Glenn W Kaatz, Richard G Brennan
    Structural and biochemical characterization of MepR, a multidrug binding transcription regulator of the Staphylococcus aureus multidrug efflux pump MepA.
    Nucleic Acids Res: 2009, 37(4);1211-24
    [PubMed:19129225] [WorldCat.org] [DOI] (I p)
    Ivan Birukou, Nam K Tonthat, Susan M Seo, Bryan D Schindler, Glenn W Kaatz, Richard G Brennan
    The molecular mechanisms of allosteric mutations impairing MepR repressor function in multidrug-resistant strains of Staphylococcus aureus.
    mBio: 2013, 4(5);e00528-13
    [PubMed:23982071] [WorldCat.org] [DOI] (I e)
    Ivan Birukou, Susan M Seo, Bryan D Schindler, Glenn W Kaatz, Richard G Brennan
    Structural mechanism of transcription regulation of the Staphylococcus aureus multidrug efflux operon mepRA by the MarR family repressor MepR.
    Nucleic Acids Res: 2014, 42(4);2774-88
    [PubMed:24293644] [WorldCat.org] [DOI] (I p)