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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0278 [new locus tag: SAUSA300_RS01490 ]
  • pan locus tag?: SAUPAN001175000
  • symbol: SAUSA300_0278
  • pan gene symbol?: esxA
  • synonym:
  • product: hypothetical protein

⊟Additional information (user-provided)[edit | edit source]

esxA: VIIb-secreted apoptosis inhibitor virulence factor EsxA

The crystal structure of EsxA can be found at the Protein Data Bank: 2VRZ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0278 [new locus tag: SAUSA300_RS01490 ]
  • symbol: SAUSA300_0278
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 330521..330814
  • length: 294
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    ATGGCAATGATTAAGATGAGTCCAGAGGAAATCAGAGCAAAATCGCAATCTTACGGGCAA
    GGTTCAGACCAAATCCGTCAAATTTTATCTGATTTAACACGTGCACAAGGTGAAATTGCA
    GCGAACTGGGAAGGTCAAGCTTTCAGCCGTTTCGAAGAGCAATTCCAACAACTTAGTCCT
    AAAGTAGAAAAATTTGCACAATTATTAGAAGAAATTAAACAACAATTGAATAGCACTGCT
    GATGCCGTTCAAGAACAAGACCAACAACTTTCTAATAATTTCGGTTTGCAATAA
    60
    120
    180
    240
    294


⊟Protein[edit | edit source]

Protein Data Bank: 2VRZ
Protein Data Bank: 2VS0

⊟General[edit | edit source]

  • locus tag: SAUSA300_0278 [new locus tag: SAUSA300_RS01490 ]
  • symbol: SAUSA300_0278
  • description: hypothetical protein
  • length: 97
  • theoretical pI: 4.32285
  • theoretical MW: 11036.2
  • GRAVY: -0.765979

⊟Function[edit | edit source]

  • TIGRFAM:
    WXG100 family type VII secretion target (TIGR03930; HMM-score: 80.2)
    and 4 more
    type VII secretion effector, TIGR04197 family (TIGR04197; HMM-score: 20.8)
    two transmembrane protein (TIGR04527; HMM-score: 18.8)
    putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 13.3)
    Genetic information processing Protein fate Protein folding and stabilization prefoldin, alpha subunit (TIGR00293; HMM-score: 10.3)
  • TheSEED  :
    • 6 kDa early secretory antigenic target ESAT-6 (EsxA)
    Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  6 kDa early secretory antigenic target ESAT-6 (EsxA)
  • PFAM:
    EsxAB (CL0352) WXG100; Proteins of 100 residues with WXG (PF06013; HMM-score: 81.5)
    and 32 more
    EspA_EspE; EspA/EspE family (PF18879; HMM-score: 22.9)
    no clan defined KxDL; Uncharacterized conserved protein (PF10241; HMM-score: 20.1)
    EsxAB (CL0352) T7SS_ESX_EspC; Excreted virulence factor EspC, type VII ESX diderm (PF10824; HMM-score: 18.5)
    no clan defined FMP23; Found in mitochondrial proteome (PF17315; HMM-score: 18.2)
    ING; Inhibitor of growth proteins N-terminal histone-binding (PF12998; HMM-score: 17.5)
    Med2; Mediator complex subunit 2 (PF11214; HMM-score: 16.5)
    Apolipoprotein (CL0725) Apolipoprotein; Apolipoprotein A1/A4/E domain (PF01442; HMM-score: 16.4)
    no clan defined Cast; RIM-binding protein of the cytomatrix active zone (PF10174; HMM-score: 16.2)
    TSNAXIP1_N; Translin-associated factor X-interacting N-terminus (PF15739; HMM-score: 15.9)
    Filament; Intermediate filament protein (PF00038; HMM-score: 15.7)
    Tup_N; Tup N-terminal (PF08581; HMM-score: 15.5)
    Spectrin (CL0743) Spectrin; Spectrin repeat (PF00435; HMM-score: 15.4)
    TPR (CL0020) COG6_N; Conserved oligomeric complex COG6, N-terminal (PF06419; HMM-score: 15.4)
    no clan defined IFT57; Intra-flagellar transport protein 57 (PF10498; HMM-score: 15.3)
    EsxAB (CL0352) EspB_PPE; ESX-1 secretion-associated protein EspB, PPE domain (PF21856; HMM-score: 15.3)
    no clan defined DUF5917; Family of unknown function (DUF5917) (PF19314; HMM-score: 15.2)
    NADAR-like (CL0787) Phage_30_3; Bacteriophage protein GP30.3 (PF08010; HMM-score: 15)
    no clan defined Mobilization_B; Mobilization protein B (PF17511; HMM-score: 14.8)
    DNA_repr_REX1B; DNA repair REX1-B (PF14966; HMM-score: 14.6)
    EsxAB (CL0352) T7SS_signal; Putative T7SS secretion signal domain (PF21725; HMM-score: 14.6)
    Perilipin_sf (CL0718) Perilipin; Perilipin family (PF03036; HMM-score: 14.3)
    no clan defined TRPM_tetra; Tetramerisation domain of TRPM (PF16519; HMM-score: 14.1)
    EsxAB (CL0352) DUF5344; Family of unknown function (DUF5344) (PF17279; HMM-score: 14.1)
    F-box (CL0271) F-box_4; F-box (PF15966; HMM-score: 13.8)
    no clan defined MscS_porin; Mechanosensitive ion channel porin domain (PF12795; HMM-score: 13.3)
    Ariadne; Ariadne domain (PF19422; HMM-score: 13.2)
    Pec_lyase-like (CL0268) FapA; Flagellar Assembly Protein A beta solenoid domain (PF03961; HMM-score: 13.1)
    no clan defined Nup49_C; Nucleoporin Nup49 C-terminal helical region (PF21121; HMM-score: 12.7)
    Eplus_motif; E+ motif (PF20430; HMM-score: 12.5)
    UPF0449; Uncharacterised protein family UPF0449 (PF15136; HMM-score: 11.9)
    MG280; Uncharacterised protein MG280 (PF23067; HMM-score: 11.2)
    Fusion_gly (CL0595) CoV_S2; Coronavirus spike glycoprotein S2 (PF01601; HMM-score: 9.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0247
    • Cytoplasmic Membrane Score: 0.0023
    • Cell wall & surface Score: 0.0012
    • Extracellular Score: 0.9718
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005097
    • TAT(Tat/SPI): 0.000636
    • LIPO(Sec/SPII): 0.001053
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWEGQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

EsxA is secreted through the Type VIIb/ESAT-6 secretion apparatus and inhibits host cell apoptosis.

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Charalampia G Korea, Giuliana Balsamo, Alfredo Pezzicoli, Christina Merakou, Simona Tavarini, Fabio Bagnoli, Davide Serruto, Meera Unnikrishnan
    Staphylococcal Esx proteins modulate apoptosis and release of intracellular Staphylococcus aureus during infection in epithelial cells.
    Infect Immun: 2014, 82(10);4144-53
    [PubMed:25047846] [WorldCat.org] [DOI] (I p)
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