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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0278 [new locus tag: SAUSA300_RS01490 ]
- pan locus tag?: SAUPAN001175000
- symbol: SAUSA300_0278
- pan gene symbol?: esxA
- synonym:
- product: hypothetical protein
esxA: VIIb-secreted apoptosis inhibitor virulence factor EsxA
The crystal structure of EsxA can be found at the Protein Data Bank: 2VRZ
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0278 [new locus tag: SAUSA300_RS01490 ]
- symbol: SAUSA300_0278
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 330521..330814
- length: 294
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914717 NCBI
- RefSeq: YP_492992 NCBI
- BioCyc: see SAUSA300_RS01490
- MicrobesOnline: 1291793 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241ATGGCAATGATTAAGATGAGTCCAGAGGAAATCAGAGCAAAATCGCAATCTTACGGGCAA
GGTTCAGACCAAATCCGTCAAATTTTATCTGATTTAACACGTGCACAAGGTGAAATTGCA
GCGAACTGGGAAGGTCAAGCTTTCAGCCGTTTCGAAGAGCAATTCCAACAACTTAGTCCT
AAAGTAGAAAAATTTGCACAATTATTAGAAGAAATTAAACAACAATTGAATAGCACTGCT
GATGCCGTTCAAGAACAAGACCAACAACTTTCTAATAATTTCGGTTTGCAATAA60
120
180
240
294
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0278 [new locus tag: SAUSA300_RS01490 ]
- symbol: SAUSA300_0278
- description: hypothetical protein
- length: 97
- theoretical pI: 4.32285
- theoretical MW: 11036.2
- GRAVY: -0.765979
⊟Function[edit | edit source]
- TIGRFAM: WXG100 family type VII secretion target (TIGR03930; HMM-score: 80.2)and 4 moretype VII secretion effector, TIGR04197 family (TIGR04197; HMM-score: 20.8)two transmembrane protein (TIGR04527; HMM-score: 18.8)putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 13.3)Protein fate Protein folding and stabilization prefoldin, alpha subunit (TIGR00293; HMM-score: 10.3)
- TheSEED :
- 6 kDa early secretory antigenic target ESAT-6 (EsxA)
- PFAM: EsxAB (CL0352) WXG100; Proteins of 100 residues with WXG (PF06013; HMM-score: 78.1)and 28 moreT7SS_ESX_EspC; Excreted virulence factor EspC, type VII ESX diderm (PF10824; HMM-score: 19.3)no clan defined Apolipoprotein; Apolipoprotein A1/A4/E domain (PF01442; HMM-score: 18.7)EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 18.3)KxDL; Uncharacterized conserved protein (PF10241; HMM-score: 18.3)DUF2130; Uncharacterized protein conserved in bacteria (DUF2130) (PF09903; HMM-score: 17.4)IFT57; Intra-flagellar transport protein 57 (PF10498; HMM-score: 16.8)Med2; Mediator complex subunit 2 (PF11214; HMM-score: 16.6)TSNAXIP1_N; Translin-associated factor X-interacting N-terminus (PF15739; HMM-score: 16.6)Filament; Intermediate filament protein (PF00038; HMM-score: 15.9)Tup_N; Tup N-terminal (PF08581; HMM-score: 15.8)Cast; RIM-binding protein of the cytomatrix active zone (PF10174; HMM-score: 15.7)ING; Inhibitor of growth proteins N-terminal histone-binding (PF12998; HMM-score: 15.1)Phage_30_3; Bacteriophage protein GP30.3 (PF08010; HMM-score: 15)DNA_repr_REX1B; DNA repair REX1-B (PF14966; HMM-score: 15)LXG; LXG domain of WXG superfamily (PF04740; HMM-score: 14.8)Mobilization_B; Mobilization protein B (PF17511; HMM-score: 14.8)PDDEXK (CL0236) RmuC; RmuC family (PF02646; HMM-score: 14.7)no clan defined CK2S; Casein Kinase 2 substrate (PF15011; HMM-score: 14.6)TRPM_tetra; Tetramerisation domain of TRPM (PF16519; HMM-score: 14.6)DUF5344; Family of unknown function (DUF5344) (PF17279; HMM-score: 14.6)Skp1; Skp1 family, dimerisation domain (PF01466; HMM-score: 13.6)Cytochrom_B562; Cytochrome b562 (PF07361; HMM-score: 12.6)MscS_porin; Mechanosensitive ion channel porin domain (PF12795; HMM-score: 11.9)OML_zippers (CL0590) LPP; Lipoprotein leucine-zipper (PF04728; HMM-score: 11.7)no clan defined UPF0449; Uncharacterised protein family UPF0449 (PF15136; HMM-score: 11.7)FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 10.8)TMPIT; TMPIT-like protein (PF07851; HMM-score: 10.5)KNOX2; KNOX2 domain (PF03791; HMM-score: 10.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 10
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005097
- TAT(Tat/SPI): 0.000636
- LIPO(Sec/SPII): 0.001053
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWEGQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
EsxA is secreted through the Type VIIb/ESAT-6 secretion apparatus and inhibits host cell apoptosis.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
- ↑ Charalampia G Korea, Giuliana Balsamo, Alfredo Pezzicoli, Christina Merakou, Simona Tavarini, Fabio Bagnoli, Davide Serruto, Meera Unnikrishnan
Staphylococcal Esx proteins modulate apoptosis and release of intracellular Staphylococcus aureus during infection in epithelial cells.
Infect Immun: 2014, 82(10);4144-53
[PubMed:25047846] [WorldCat.org] [DOI] (I p)