PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0361 [new locus tag: SAUSA300_RS01915 ]
• pan locus tag^?: SAUPAN001906000
• symbol: SAUSA300_0361
• pan gene symbol^?: parB
• synonym:
• product: ParB-like partition protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0361 [new locus tag: SAUSA300_RS01915 ]
• symbol: SAUSA300_0361
• product: ParB-like partition protein
• replicon: chromosome
• strand: +
• coordinates: 415027..415872
• length: 846
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914812 NCBI
• RefSeq: YP_493075 NCBI
• BioCyc: see SAUSA300_RS01915
• MicrobesOnline: 1291876 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
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  841
  
  GTGAGTGAATTGTCAAAAAGTGAAGATCAACGTATTACTAAAACAAAAGATGAACAAATT
  AAGCAAATAGATATATCGGATATCAAACCGAATCCGTATCAGCCCCGAAAAACTTTCGAT
  GAAAATCATTTAAATGATTTGGCAGATTCAATTAAGCAATATGGAATTTTGCAACCAATT
  GTGCTTAGAAAAACAGTTCAAGGTTATTACATTGTAGTTGGTGAAAGAAGGTTTAGAGCT
  TCGAAAATTGCTGGTCTAAAATACGTATCAGCGATTATCAAAGATTTAACAGATGAAGAT
  ATGATGGAACTGGCGGTCATCGAAAATTTACAACGAGAAGACTTAAATGCGATTGAAGAA
  GCTGAAAGTTATCAACGTTTGATGACAGATTTGAAAATTACACAACAAGAAGTAGCGAAA
  CGATTGAGTAAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAA
  AAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTGCACATGGACGAACGTTATTG
  GCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGG
  AGTGTCAGATATTTAGAAAACCATGTTAATGAATTAAAAAATGTTTCGTCAAAGTCGGAA
  ACAGACAAAGTAGATATAACTAAGCCTAAATTTATAAAGCAGCAAGAACGACAGTTGCGA
  GAACAGTATGGTACCAAAGTAGATATATCAATAAAAAAATCGGTTGGTAAAATCTCATTT
  GAGTTTGATTCACAAGAAGATTTTGTGAGAATAATTGAACAATTAAATCGTAGGTATGGT
  AAATAG
  60
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  846
  

⊟Protein

⊟General

• locus tag: SAUSA300_0361 [new locus tag: SAUSA300_RS01915 ]
• symbol: SAUSA300_0361
• description: ParB-like partition protein
• length: 281
• theoretical pI: 10.0114
• theoretical MW: 32855.6
• GRAVY: -0.712456

⊟Function

• TIGRFAM:
  nucleoid occlusion protein (TIGR04285; HMM-score: 248.1)
  and 3 more
  ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 195.1)
  PRTRC system ParB family protein (TIGR03734; HMM-score: 118.7)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepB (TIGR03454; HMM-score: 70.9)
• TheSEED  :
  • Chromosome (plasmid) partitioning protein ParB
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Chromosome (plasmid) partitioning protein ParB
  and 1 more
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning  Chromosome (plasmid) partitioning protein ParB
• PFAM:
  ParBc (CL0248) ParBc; ParB-like nuclease domain (PF02195; HMM-score: 94.5)
  and 12 more
  no clan defined Latarcin; Latarcin precursor (PF10279; HMM-score: 22.3)
  PDDEXK (CL0236) DUF1016; Protein of unknown function (DUF1016) (PF06250; HMM-score: 17.4)
  HTH (CL0123) KorB; KorB domain (PF08535; HMM-score: 17.1)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 16.7)
  MarR_2; MarR family (PF12802; HMM-score: 13.7)
  MerR_2; MerR HTH family regulatory protein (PF13591; HMM-score: 13.3)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13)
  no clan defined Statherin; Statherin (PF03875; HMM-score: 12.9)
  HTH (CL0123) TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 12.6)
  no clan defined CbeA_antitoxin; CbeA_antitoxin, type IV, cytoskeleton bundling-enhancing factor A (PF06154; HMM-score: 12.5)
  HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.1)
  HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 9.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007656
  • TAT(Tat/SPI): 0.000789
  • LIPO(Sec/SPII): 0.000953
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161316 NCBI
• RefSeq: YP_493075 NCBI
• UniProt: A0A0H2XGE1 UniProt

⊟Protein sequence

• MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHLNDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMMELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPKKIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSKSETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLNRRYGK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications