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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0432 [new locus tag: SACOL_RS02175 ]
  • pan locus tag?: SAUPAN001906000
  • symbol: SACOL0432
  • pan gene symbol?: parB
  • synonym:
  • product: spoOJ protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0432 [new locus tag: SACOL_RS02175 ]
  • symbol: SACOL0432
  • product: spoOJ protein
  • replicon: chromosome
  • strand: +
  • coordinates: 438948..439793
  • length: 846
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGAGTGAATTGTCAAAAAGTGAAGATCAACGTATTACTAAAACAAAAGATGAACAAATT
    AAGCAAATAGATATATCGGATATCAAACCGAATCCGTATCAGCCCCGAAAAACTTTCGAT
    GAAAATCATTTAAATGATTTGGCAGATTCAATTAAGCAATATGGAATTTTGCAACCAATT
    GTGCTTAGAAAAACAGTTCAAGGTTATTACATTGTAGTTGGTGAAAGAAGGTTTAGAGCT
    TCGAAAATTGCTGGTCTAAAATACGTATCAGCGATTATCAAAGATTTAACAGATGAAGAT
    ATGATGGAACTGGCGGTCATCGAAAATTTACAACGAGAAGACTTAAATGCGATTGAAGAA
    GCTGAAAGTTATCAACGTTTGATGACAGATTTGAAAATTACACAACAAGAAGTAGCGAAA
    CGATTGAGTAAGTCGCGCCCGTATATAGCGAATATGTTGAGGTTATTACATTTGCCGAAA
    AAGATTGCTGACATGGTAAAAGATGGGCGACTGACAAGTGCACATGGACGAACGTTATTG
    GCAATTAAAGATGAACAACAAATGCTTAGGTTAGCGAAACGGGTTGTTAAAGAAAAGTGG
    AGTGTCAGATATTTAGAAAACCATGTTAATGAATTAAAAAATGTTTCGTCAAAGTCGGAA
    ACAGACAAAGTAGATATAACTAAGCCTAAATTTATAAAGCAGCAAGAACGACAGTTGCGA
    GAACAGTATGGTACCAAAGTAGATATATCAATAAAAAAATCGGTTGGTAAAATCTCATTT
    GAGTTTGATTCACAAGAAGATTTTGTGAGAATAATTGAACAATTAAATCGTAGGTATGGT
    AAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    846

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0432 [new locus tag: SACOL_RS02175 ]
  • symbol: SACOL0432
  • description: spoOJ protein
  • length: 281
  • theoretical pI: 10.0114
  • theoretical MW: 32855.6
  • GRAVY: -0.712456

⊟Function[edit | edit source]

  • TIGRFAM:
    nucleoid occlusion protein (TIGR04285; HMM-score: 248.1)
    and 3 more
    ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 195.1)
    PRTRC system ParB family protein (TIGR03734; HMM-score: 118.7)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepB (TIGR03454; HMM-score: 70.9)
  • TheSEED  :
    • Chromosome (plasmid) partitioning protein ParB
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Chromosome (plasmid) partitioning protein ParB
    and 1 more
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning  Chromosome (plasmid) partitioning protein ParB
  • PFAM:
    ParBc (CL0248) ParBc; ParB-like nuclease domain (PF02195; HMM-score: 94.5)
    and 12 more
    no clan defined Latarcin; Latarcin precursor (PF10279; HMM-score: 22.3)
    PDDEXK (CL0236) DUF1016; Protein of unknown function (DUF1016) (PF06250; HMM-score: 17.4)
    HTH (CL0123) KorB; KorB domain (PF08535; HMM-score: 17.1)
    HTH_3; Helix-turn-helix (PF01381; HMM-score: 16.7)
    MarR_2; MarR family (PF12802; HMM-score: 13.7)
    MerR_2; MerR HTH family regulatory protein (PF13591; HMM-score: 13.3)
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13)
    no clan defined Statherin; Statherin (PF03875; HMM-score: 12.9)
    HTH (CL0123) TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 12.6)
    no clan defined CbeA_antitoxin; CbeA_antitoxin, type IV, cytoskeleton bundling-enhancing factor A (PF06154; HMM-score: 12.5)
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.1)
    HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007656
    • TAT(Tat/SPI): 0.000789
    • LIPO(Sec/SPII): 0.000953
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHLNDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMMELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPKKIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSKSETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLNRRYGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell: 50 [2]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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