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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0417 [new locus tag: SAUSA300_RS02235 ]
- pan locus tag?: SAUPAN002148000
- symbol: SAUSA300_0417
- pan gene symbol?: lpl7
- synonym:
- product: tandem lipoprotein
tilA8 (lpl8): tandem TslA phospholipase immunity lipoprotein paralogue TilA8
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0417 [new locus tag: SAUSA300_RS02235 ]
- symbol: SAUSA300_0417
- product: tandem lipoprotein
- replicon: chromosome
- strand: +
- coordinates: 469538..470353
- length: 816
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913723 NCBI
- RefSeq: YP_493130 NCBI
- BioCyc: see SAUSA300_RS02235
- MicrobesOnline: 1291932 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGATGGGGTATATAAAAAGAATGGCTTTATACATGAGTGTATTTCTTTTAATCATTTTT
ATTGTTGGATGTCGAAATATGAAAGATGAACAGAAAAAAGAAGAACAAACAAATAAAACA
GATTCAAAAGAAGAACAAATCAAAAAGAGTTTTGAGAAAACATTAGATATGTATCCAATT
AAGAATCTCGAGGAGTTATACGACAAAGAAGGATACCGAGATGGCGAATTTAAAAAGGGT
GATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTG
AGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGACATTAT
TTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAA
ATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGA
ATAGAAAACTTTAAATTTTTTGGACAATATGCAAACCTTAAAGAATTGAAAAACTACAAC
AATGGTGATGTCTCAATTAATGAGAATGTTCCAAGTTATGACGCAAAATTTAAAATGAGC
AATAAAGATGAAAATGTTAAGCAATTAAGAAGTCGTTATAATATTCCTACTGATAAAGCA
CCGGTATTAAAAATGCATATTGATGGTAATTTGAAAGGAAGTTCTGTGGGTTATAAAAAG
TTGGAAATTGACTTTTCAAAAGGTGGAAAAAGCGATTTGTCAGTAATAGATTCTTTGAAT
TTCCAGCCGGCGAAGGTAGATGAAGATGATGAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0417 [new locus tag: SAUSA300_RS02235 ]
- symbol: SAUSA300_0417
- description: tandem lipoprotein
- length: 271
- theoretical pI: 9.6824
- theoretical MW: 31631
- GRAVY: -0.906273
⊟Function[edit | edit source]
- TIGRFAM: Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 421.6)
- TheSEED :
- Tandem lipoprotein within Pathogenicity island
- PFAM: LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 370.5)and 2 moreAdhesin (CL0204) DUF2807; Putative auto-transporter adhesin, head GIN domain (PF10988; HMM-score: 13)no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 12.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helix: 1
- LocateP: Lipid anchored
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPII)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: IFIVGCR
- SignalP: Signal peptide LIPO(Sec/SPII) length 23 aa
- SP(Sec/SPI): 0.000415
- TAT(Tat/SPI): 0.000057
- LIPO(Sec/SPII): 0.999193
- Cleavage Site: CS pos: 23-24. IVG-CR. Pr: 0.9998
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MMGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEEQIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRIENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SaeR (activation) regulon
SaeR (TF) important in Virulence; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
This locus is highly prone to recombination leading to gene duplication / loss. Gene nomenclature is confusing and appears to be numbered sequentially by position in the genome and is not intended to show homology between strains (e.g. the first instance of tilA/lpl in COL may have the greatest homology with the sixth instance in FPR3757 but the gene names would still be different [i.e. tilA1/lpl1 and tilA6/lpl6 in COL and FPR3757, respectively]).
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
- ↑ Stephen R Garrett, Nicole Mietrach, Justin Deme, Alina Bitzer, Yaping Yang, Fatima R Ulhuq, Dorothee Kretschmer, Simon Heilbronner, Terry K Smith, Susan M Lea, Tracy Palmer
A type VII-secreted lipase toxin with reverse domain arrangement.
Nat Commun: 2023, 14(1);8438
[PubMed:38114483] [WorldCat.org] [DOI] (I e)Stephen R Garrett, Giuseppina Mariano, Jo Dicks, Tracy Palmer
Homologous recombination between tandem paralogues drives evolution of a subset of type VII secretion system immunity genes in firmicute bacteria.
Microb Genom: 2022, 8(8);
[PubMed:35960642] [WorldCat.org] [DOI] (I p)