PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1404 [new locus tag: SAUSA300_RS07660 ]
• pan locus tag^?: SAUPAN001834000
• symbol: SAUSA300_1404
• pan gene symbol^?: —
• synonym:
• product: phiSLT ORF 563-like protein, terminase, large subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1404 [new locus tag: SAUSA300_RS07660 ]
• symbol: SAUSA300_1404
• product: phiSLT ORF 563-like protein, terminase, large subunit
• replicon: chromosome
• strand: -
• coordinates: 1570074..1571765
• length: 1692
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913877 NCBI
• RefSeq: YP_494101 NCBI
• BioCyc: see SAUSA300_RS07660
• MicrobesOnline: 1292919 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1681
  
  GTGACTATTAAAGTTTTAAATGAACCTTCACCAAAACTATTAACAACATGGTATGCAGAG
  CAAGTCACTCAAGGGAAAATAAAAACAAGCAAATATGTTAAAAAAGAATGTGAGAGACAC
  CTTAGATATCTAGAAAATGGAGGTAAATGGGTATTTGATGAAGAATTAGCGCACCGTCCT
  ATTCGATTCATAGAAAAGTTTTGTAAACCTTCCAAAGGATCTAAACGTCAACTTGTATTA
  CAACCATGGCAACATTTTATTATTGGCAGTTTGTTTGGTTGGGTTCATAAAGAAACAAAA
  CTGCGCAGGTTTAAAGAAGCTTTGATATTTATGGGGCGAAAAAATGGTAAAACAACTACT
  ATATCTGGTGTTGCTAACTATGCTGTTTCTCAAGATGGAGAAAACGGCGCTGAAATCCAT
  CTTTTAGCAAACGTAATGAAACAAGCTAGAATATTATTCGATGAATCTAAGGCGATGATA
  AAAGCTAGCCCAAAGCTTGATAAAAATTTCAGAACATTAAGAGATGAAATCCATTATGAC
  GCAACGATATCAAAAATTATGCCCCAAGCATCAGATAGCGATAAGTTAGATGGATTGAAT
  ACACACATGGGGATTTTTGATGAAATTCATGAATTTAAAGACTATAAATTGATTTCAGTT
  ATAAAAAACTCAAGAGCTGCAAGGTTACAACCTCTTCTCATCTACATTACGACAGCAGGG
  TATCAATTAGATGGTCCACTTGTTGATATGGTAGAAGCGGGAAGAGACACCTTAGATCAA
  ATCATAGAAGACGAAAGAACTTTTTATTATTTAGCATCTTTGGATGATGACGATGATATT
  AATGATTCGTCGAACTGGATAAAAGCAAATCCCAACTTAGGTGTCTCTATAAATTTAGAT
  GAGATGAAAGAAGAGTGGGAAAAAGCTAAGAGAACACCAGCTGAACGTGGAGATTTTATA
  ACCAAAAGGTTTAATATCTTTGCTAATAATGACGAGATGAGTTTTATTGATTACCCAACA
  CTCCAAAAAAATAATGAAATTGTTTCTTTAGAAGAGCTGGAAGGCAGACCATGCACGATT
  GGTTATGATTTATCAGAAACAGAGGACTTTACAGCCGCGTGTGCTACTTTTGCGTTAGAT
  AATGGTAAAGTTGCAGTTTTATCGCATTCATGGATTCCTAAGCACAAAGTTGAATATTCT
  AACGAAAAAATACCCTATAGAGAATGGGAAGAAGATGGCTTATTAACAGTGCAAGATAAG
  CCTTATATTGACTACCAAGATGTTTTAAATTGGATAATTAAGATGAATGAGCATTATGTA
  GTAGAAAAAATTACTTATGATAGAGCGAACGCATTCAAACTAAATCAAGAGTTAAAAAAT
  TACGGGTTTGAAACGGAAGAAACAAGACAAGGAGCTTTGACCTTGAGCCCTGCATTGAAG
  GATTTAAAAGAAATGTTTTTAGATGGGAAAATAATATTTAATAATAATCCTTTAATGAAA
  TGGTATATCAATAATGTTCAGTTGAAACTAGACAGAAACGGAAACTGGTTGCCGTCTAAG
  CAAAGCAGATATCGTAAAATAGATGGCTTTGCAGCATTTTTAAACACATATACAGATATT
  ATGAATAAAGTTGTTTCTGACAAGGGTGAAGGAAACATAGAATTTATTAGTATTAAAGAT
  ATAATGCGTTAA
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  1692
  

⊟Protein

⊟General

• locus tag: SAUSA300_1404 [new locus tag: SAUSA300_RS07660 ]
• symbol: SAUSA300_1404
• description: phiSLT ORF 563-like protein, terminase, large subunit
• length: 563
• theoretical pI: 6.18003
• theoretical MW: 65223.8
• GRAVY: -0.554529

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage terminase large subunit
• PFAM:
  P-loop_NTPase (CL0023) Terminase_1; Phage Terminase (PF03354; HMM-score: 276.5)
  and 3 more
  no clan defined Tail_tube; Phage tail tube protein (PF10618; HMM-score: 13.9)
  CagD; Pathogenicity island component CagD (PF16567; HMM-score: 13.9)
  COP23; Circadian oscillating protein COP23 (PF14218; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011278
  • TAT(Tat/SPI): 0.000308
  • LIPO(Sec/SPII): 0.003165
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162180 NCBI
• RefSeq: YP_494101 NCBI
• UniProt: A0A0H2XK51 UniProt

⊟Protein sequence

• MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYLENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLRRFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMIKASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLISVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDDDDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMSFIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWIPKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRANAFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQLKLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1401 < SAUSA300_1402 < SAUSA300_1403 < SAUSA300_1404 < SAUSA300_1405

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications