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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1958 [new locus tag: SAUSA300_RS10740 ]
- pan locus tag?: SAUPAN001395000
- symbol: SAUSA300_1958
- pan gene symbol?: ssb2
- synonym:
- product: single-strand binding protein
ssb : prophage ssDNA-binding protein [1]
Single-stranded DNA-binding proteins (SSBs) protect 3'-ssDNA overhangs from degradation and are essential components of single-stranded DNA recombinases. There is powerful evolutionary pressure for structural diversity as SSBs are frequent targets for CRISPR-mediated anti-phage defenses and several SSB paralogues exist. Prophage SSBs are typically paired with a single-strand DNA exonuclease and a single-strand DNA recombinase.
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1958 [new locus tag: SAUSA300_RS10740 ]
- symbol: SAUSA300_1958
- product: single-strand binding protein
- replicon: chromosome
- strand: -
- coordinates: 2115561..2116031
- length: 471
- essential: unknown
⊟Accession numbers[edit | edit source]
- Gene ID: 3914250 NCBI
- RefSeq: YP_494609 NCBI
- BioCyc: see SAUSA300_RS10740
- MicrobesOnline: 1293473 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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421ATGCTAAACAGAACAATATTAGTTGGTCGTTTAACTAGAGACCCAGAATTAAGAACCACT
CAAAATGGTGTAAATGTAGCATCATTCACATTAGCAGTTAACCGTACATTTACAAATGCA
CAAGGCGAGCGCGAGGCAGACTTTATTAATATCATCGTATTTAAAAAACAAGCAGAGAAC
GTTAATAAATACCTATCTAAAGGATCGTTGGCGGGCGTAGATGGTAGGTTACAAACGCGG
AACTATGAAAATAAGGAAGGTCAACGTGTATACGTTACGGAAGTTATTGCTGATAGTATT
CAATTTTTAGAACCGAAAAACTCAAATGACACTCAACAAGATTTATATCAACAACAAGTA
CAACAAACACGTGGACAATCGCAATATTCAAATAACAAACCAGTAAAAGATAATCCGTTT
GCGAATGCAAATGGTCCGATTGAACTAAATGATGATGATTTACCATTCTAA60
120
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471
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1958 [new locus tag: SAUSA300_RS10740 ]
- symbol: SAUSA300_1958
- description: single-strand binding protein
- length: 156
- theoretical pI: 5.26974
- theoretical MW: 17668.4
- GRAVY: -0.807692
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair single-stranded DNA-binding protein (TIGR00621; HMM-score: 153.5)and 1 moreDNA metabolism DNA replication, recombination, and repair primosomal replication protein PriB (TIGR04418; HMM-score: 26.4)
- TheSEED :
- Single-stranded DNA-binding protein
and 1 more - PFAM: OB (CL0021) SSB; Single-strand binding protein family (PF00436; HMM-score: 130.3)and 3 moreSSB_1; Another single-strand binding protein family (PF22657; HMM-score: 21.5)tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 19.3)no clan defined DUF6535; Family of unknown function (DUF6535) (PF20153; HMM-score: 13.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9162
- Cytoplasmic Membrane Score: 0.0019
- Cell wall & surface Score: 0.0013
- Extracellular Score: 0.0806
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014241
- TAT(Tat/SPI): 0.002993
- LIPO(Sec/SPII): 0.002245
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGEREADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQFLEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maan M Neamah, Ignacio Mir-Sanchis, María López-Sanz, Sonia Acosta, Ignacio Baquedano, Andreas F Haag, Alberto Marina, Silvia Ayora, José R Penadés
Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus.
Nucleic Acids Res: 2017, 45(11);6507-6519
[PubMed:28475766] [WorldCat.org] [DOI] (I p)Anne Lopes, Jihane Amarir-Bouhram, Guilhem Faure, Marie-Agnès Petit, Raphaël Guerois
Detection of novel recombinases in bacteriophage genomes unveils Rad52, Rad51 and Gp2.5 remote homologs.
Nucleic Acids Res: 2010, 38(12);3952-62
[PubMed:20194117] [WorldCat.org] [DOI] (I p)