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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2157 [new locus tag: SAUSA300_RS11880 ]
- pan locus tag?: SAUPAN005595000
- symbol: SAUSA300_2157
- pan gene symbol?: cobB
- synonym:
- product: NAD-dependent deacetylase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2157 [new locus tag: SAUSA300_RS11880 ]
- symbol: SAUSA300_2157
- product: NAD-dependent deacetylase
- replicon: chromosome
- strand: +
- coordinates: 2334114..2334845
- length: 732
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3915293 NCBI
- RefSeq: YP_494793 NCBI
- BioCyc: see SAUSA300_RS11880
- MicrobesOnline: 1293672 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721TTGAAACATGATTTAGAGACACTAAAACATATTATAGATAGTTCGAATCGTATTACATTT
TTTACAGGTGCTGGTGTATCTGTCGCAAGTGGCGTTCCAGATTTCCGTTCAATGGGTGGC
TTATTTGATGAAATTTCAAAAGATGGGCTTTCGCCAGAATACTTGTTAAGCCGTGATTAT
TTAGAAGATGATCCTGAAGGTTTTATCAATTTCTGCCATAAGCGCTTACTATTTGTCGAT
ACGATGCCCAATATCGTTCATGATTGGATTGCAAAATTAGAACGTAATCAACAATCTTTA
GGTGTCATCACGCAAAATATCGATGGTTTGCACTCAGACGCTGGAAGTCAACATGTTGAT
GAATTACATGGCACGTTAAATCGTTTTTATTGTAATGTATGTCATAAATCTTATACGAAG
TCAGATGTTATTGATAGAACTTTGAAACATTGTGATAATTGTGGTGGTGCCATTCGACCG
GACATCGTATTATACGGTGAAATGTTAGATCAACCTACCATCATAAGGGCATTAAATAAA
ATTGAACATGCTGACACCCTTGTTGTACTAGGTTCATCACTCGTTGTACAACCTGCCGCA
GGATTAATATCACATTTTAAAGGCGACAATTTAATTATTATCAATAAAGATCGAACACCT
TATGATAGCGACGCTACTTTGGTCATACATGACGACATGGTTTCCGTTGTAAAATCATTA
ATGACAGAGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2157 [new locus tag: SAUSA300_RS11880 ]
- symbol: SAUSA300_2157
- description: NAD-dependent deacetylase
- length: 243
- theoretical pI: 5.27118
- theoretical MW: 27051.5
- GRAVY: -0.152675
⊟Function[edit | edit source]
- reaction: EC 3.5.1.-? ExPASyNAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein?
- TIGRFAM:
- TheSEED :
- NAD-dependent protein deacetylase of SIR2 family
Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate NAD-dependent protein deacetylase of SIR2 familyand 1 more - PFAM: FAD_DHS (CL0085) SIR2; Sir2 family (PF02146; HMM-score: 184.3)and 3 moreC2H2-zf (CL0361) zf-BED; BED zinc finger (PF02892; HMM-score: 12.7)Globin (CL0090) Protoglobin; Protoglobin (PF11563; HMM-score: 12)Zn_Beta_Ribbon (CL0167) Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 11.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Zn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.060877
- TAT(Tat/SPI): 0.053145
- LIPO(Sec/SPII): 0.021778
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGAIRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSLMTE
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_0523 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_2203 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_0533 (tuf) elongation factor Tu [1] (data from MRSA252) SAUSA300_0844 hypothetical protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)