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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1999 [new locus tag: SA_RS11505 ]
  • pan locus tag?: SAUPAN005595000
  • symbol: SA1999
  • pan gene symbol?: cobB
  • synonym:
  • product: NAD-dependent deacetylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1999 [new locus tag: SA_RS11505 ]
  • symbol: SA1999
  • product: NAD-dependent deacetylase
  • replicon: chromosome
  • strand: +
  • coordinates: 2270399..2271130
  • length: 732
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    TTGAGAAATGATTTAGAGACATTAAAACATATTATAGATAGTTCGAATCGTATTACATTT
    TTCACAGGTGCTGGTGTATCTGTCGCAAGTGGCGTTCCAGATTTCCGTTCAATGGGTGGC
    TTATTTGATGAAATTTCAAAAGATGGACTTTCGCCAGAATACTTGTTAAGCCGTGATTAT
    TTAGAAGATGATCCTGAAGGTTTTATCAATTTCTGCCATAAGCGTTTACTATTTGTCGAT
    ACGAAGCCTAATATCGTTCATGATTGGATTGCAAAATTAGAACGTAATCAACAATCTTTA
    GGTGTCATCACGCAAAATATCGATGGTTTGCACTCAGACGCTGGAAGTCAACATGTTGAT
    GAATTACATGGCACGTTAAATCGTTTTTATTGTAATGCATGTCATAAATCTTATACGAAG
    TCAGATGTTATTGATAGAACTTTGAAACATTGTGATAATTGTGGTGGTGCCATTCGACCG
    GACATCGTATTATACGGTGAAATGTTAGATCAACCTACCATCATAAGGGCATTAAATAAA
    ATTGAACATGCTGACACCCTTGTTGTACTAGGTTCATCACTCGTTGTACAACCTGCCGCA
    GGATTAATATCACATTTTAAAGGCGACAATTTAATTATTATCAATAAAGATCGAACACCT
    TATGATAGCGACGCTACTTTGGTCATACATGACGACATGGTTTCCGTTGTAAAATCATTA
    ATGACAGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    732

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1999 [new locus tag: SA_RS11505 ]
  • symbol: SA1999
  • description: NAD-dependent deacetylase
  • length: 243
  • theoretical pI: 5.28239
  • theoretical MW: 27025.4
  • GRAVY: -0.190123

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.-?  ExPASy
    NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein?
  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions putative regulatory protein, FmdB family (TIGR02605; HMM-score: 11.8)
  • TheSEED  :
    • NAD-dependent protein deacylase (EC 2.3.1.286)
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate  NAD-dependent protein deacetylase of SIR2 family
    and 1 more
    Stress Response Oxidative stress Redox-dependent regulation of nucleus processes  NAD-dependent protein deacetylase of SIR2 family
  • PFAM:
    FAD_DHS (CL0085) SIR2; Sir2 family (PF02146; HMM-score: 184.9)
    and 4 more
    Zn_Beta_Ribbon (CL0167) Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 13.7)
    Nudix_N_2; Nudix N-terminal (PF14803; HMM-score: 12.8)
    Multiheme_cytos (CL0317) Cytochrome_C7; Cytochrome c7 and related cytochrome c (PF14522; HMM-score: 11.7)
    Zn_Beta_Ribbon (CL0167) zf-TFIIB; Transcription factor zinc-finger (PF13453; HMM-score: 10.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.070865
    • TAT(Tat/SPI): 0.067177
    • LIPO(Sec/SPII): 0.024026
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGAIRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSLMTE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA0496(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA0802hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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