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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS11505 [old locus tag: SA1999 ]
  • pan locus tag?: SAUPAN005595000
  • symbol: SA_RS11505
  • pan gene symbol?: cobB
  • synonym:
  • product: NAD-dependent protein deacylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS11505 [old locus tag: SA1999 ]
  • symbol: SA_RS11505
  • product: NAD-dependent protein deacylase
  • replicon: chromosome
  • strand: +
  • coordinates: 2270390..2271130
  • length: 741
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2270390..2271130) NCBI
  • BioCyc: SA_RS11505 BioCyc
  • MicrobesOnline: see SA1999

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGTTTATTTTGAGAAATGATTTAGAGACATTAAAACATATTATAGATAGTTCGAATCGT
    ATTACATTTTTCACAGGTGCTGGTGTATCTGTCGCAAGTGGCGTTCCAGATTTCCGTTCA
    ATGGGTGGCTTATTTGATGAAATTTCAAAAGATGGACTTTCGCCAGAATACTTGTTAAGC
    CGTGATTATTTAGAAGATGATCCTGAAGGTTTTATCAATTTCTGCCATAAGCGTTTACTA
    TTTGTCGATACGAAGCCTAATATCGTTCATGATTGGATTGCAAAATTAGAACGTAATCAA
    CAATCTTTAGGTGTCATCACGCAAAATATCGATGGTTTGCACTCAGACGCTGGAAGTCAA
    CATGTTGATGAATTACATGGCACGTTAAATCGTTTTTATTGTAATGCATGTCATAAATCT
    TATACGAAGTCAGATGTTATTGATAGAACTTTGAAACATTGTGATAATTGTGGTGGTGCC
    ATTCGACCGGACATCGTATTATACGGTGAAATGTTAGATCAACCTACCATCATAAGGGCA
    TTAAATAAAATTGAACATGCTGACACCCTTGTTGTACTAGGTTCATCACTCGTTGTACAA
    CCTGCCGCAGGATTAATATCACATTTTAAAGGCGACAATTTAATTATTATCAATAAAGAT
    CGAACACCTTATGATAGCGACGCTACTTTGGTCATACATGACGACATGGTTTCCGTTGTA
    AAATCATTAATGACAGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    741

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS11505 [old locus tag: SA1999 ]
  • symbol: SA_RS11505
  • description: NAD-dependent protein deacylase
  • length: 246
  • theoretical pI: 5.28239
  • theoretical MW: 27398.9
  • GRAVY: -0.142683

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.-?  ExPASy
    NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein?
  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions putative regulatory protein, FmdB family (TIGR02605; HMM-score: 11.8)
  • TheSEED: see SA1999
  • PFAM:
    FAD_DHS (CL0085) SIR2; Sir2 family (PF02146; HMM-score: 184.8)
    and 4 more
    Zn_Beta_Ribbon (CL0167) Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 13.7)
    Nudix_N_2; Nudix N-terminal (PF14803; HMM-score: 12.7)
    Multiheme_cytos (CL0317) Cytochrome_C7; Cytochrome c7 and related cytochrome c (PF14522; HMM-score: 11.6)
    Zn_Beta_Ribbon (CL0167) zf-TFIIB; Transcription factor zinc-finger (PF13453; HMM-score: 10.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.045364
    • TAT(Tat/SPI): 0.061573
    • LIPO(Sec/SPII): 0.010598
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGAIRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSLMTE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS0291050S ribosomal protein L1  [1] (data from MRSA252)
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS04575NADH dehydrogenase  [1] (data from MRSA252)
    SA_RS1176050S ribosomal protein L4  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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