PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12360 [old locus tag: SA2152 ]
• pan locus tag^?: SAUPAN005871000
• symbol: SA_RS12360
• pan gene symbol^?: hssS
• synonym:
• product: two-component sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12360 [old locus tag: SA2152 ]
• symbol: SA_RS12360
• product: two-component sensor histidine kinase
• replicon: chromosome
• strand: +
• coordinates: 2417581..2418954
• length: 1374
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2417581..2418954) NCBI
• BioCyc: SA_RS12360 BioCyc
• MicrobesOnline: see SA2152

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTTAAAACACTCTATGCTAGAATTGCGATTTATTCCATTACGGTTATTTTATTTAGT
  GCATTAATAAGTTTTGTATTAACAAATGTTTACTATCATTATAATTTAAAAGCATCTAAT
  GACGCGAAAATCATGAAGACACTTAAAGAAGCAAGACAATATGAACAATCAGCTAAACCA
  ACGCACATTCAACAATATTTCAAACATTTAGGGCAAATGAATTACCAAATAATGACCGTT
  GATCAAAAAGGTCATAAGACATTTTATGGGGAGCCATTTAGAGAAGATACTTTATCACAA
  AATGCTATTAACAACGTATTAAATAATAAAGATTACCATGGTATTAAAGATAAACCATTC
  GCATTATTCGTAACTGGATTTTTCGACAATGTAACAGATAATACTGTTGGTATTAACTTT
  AAGACAAAAGACGGTTCAATAGCAGTATTCATGCGTCCAGATATTGGAGAAACATTTAGC
  GAGTTTAGAACATTCTTAGCCGTCTTGTTAATGTTGTTATTATTTATCTCGATTTCATTA
  GTTATCGCATCAACCTATTCAATCATTCGTCCAGTAAAAAAGTTAAAGCTAGCGACCGAA
  CGCTTAATTGATGGTGATTTTGAAACACCTATCAAACAAACACGCAAAGATGAAATTGGA
  ACATTACAGTATCACTTTAATAAGATGAGAGAGTCATTGGGACAAGTTGATCAAATGAGG
  CAGCATTTTGTACAAAATGTATCTCATGAAATCAAAACACCATTAACACATATTCATCAT
  TTATTAAGCGAGTTACAACAAACTTCTGATAAAACATTAAGACAACAATATATAAATGAT
  ATTTATACCATTACAACGCAGCTGAGCGGATTAACAACTGAATTGTTACTCTTATCCGAA
  TTAGATAATCACCAACATTTATTATTCGATGATAAGATTCAAGTCGATCAACTCATTAAA
  GATATCATTCGACATGAACAATTTGCTGCTGATGAAAAGTCGTTAATCATTTTAGCTGAC
  CTAGAATCAATCAATTTCCTAGGAAATCAACGTTTGTTACATCAAGCATTGAGTAATTTA
  TTAATAAATGCTATTAAGTATACAGATGTTGGTGGTGCCATTGACATCGCTTTGCAACAT
  AGTCACAACAATATCATTTTCACAATAAGTAATGATGGTTCACCAATTAGTCCACAAGCT
  GAAGCACGTTTATTTGAACGTTTTTATAAAGTGAGCAAACATGATAATAGCAATGGTTTA
  GGTCTTGCAATTACAAAATCCATCATTGAATTGCATCATGGTACAATCCAATTCACACAA
  AGTAACGAGTATGTAACCACCTTTACTATTACACTGCCAAATAATTCACATTAA
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⊟Protein

⊟General

• locus tag: SA_RS12360 [old locus tag: SA2152 ]
• symbol: SA_RS12360
• description: two-component sensor histidine kinase
• length: 457
• theoretical pI: 7.26925
• theoretical MW: 52385.5
• GRAVY: -0.198687

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 158.5)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 149.6)
  and 6 more
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 105.2)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 85.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 46.8)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 46.8)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 17.4)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 17.4)
• TheSEED: see SA2152
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 70.9)
  and 10 more
  no clan defined HAMP; HAMP domain (PF00672; HMM-score: 36.2)
  His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 32.4)
  HATPase_c_5; GHKL domain (PF14501; HMM-score: 28.3)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 15)
  no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 14.4)
  DUF2737; Protein of unknown function (DUF2737) (PF10930; HMM-score: 13.4)
  DUF4199; Protein of unknown function (DUF4199) (PF13858; HMM-score: 11.6)
  Blo-t-5; Mite allergen Blo t 5 (PF11642; HMM-score: 10.6)
  SAPIS-gp6; Pathogenicity island protein gp6 in Staphylococcus (PF16722; HMM-score: 9.4)
  IT (CL0182) DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 6.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.09055
  • TAT(Tat/SPI): 0.000758
  • LIPO(Sec/SPII): 0.027154
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 446399483 NCBI
• RefSeq: WP_000477338 NCBI
• UniProt: see SA2152

⊟Protein sequence

• MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKIMKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNAINNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFSEFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDEIGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQYINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKSLIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISNDGSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTITLPNNSH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications