PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS13045 [old locus tag: SA2272 ]
• pan locus tag^?: SAUPAN006071000
• symbol: SA_RS13045
• pan gene symbol^?: —
• synonym:
• product: membrane protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS13045 [old locus tag: SA2272 ]
• symbol: SA_RS13045
• product: membrane protein
• replicon: chromosome
• strand: -
• coordinates: 2547293..2548618
• length: 1326
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2547293..2548618) NCBI
• BioCyc: SA_RS13045 BioCyc
• MicrobesOnline: see SA2272

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGAACAAAACTAAATATCAAATAAATTCTGACAATATAAAGAGTAACTCTGAAGAAACA
  AGCGCAATATCTAGTATAAGTTATGAAATAGAAAACGCAAATAATAACGATTTAAATAAT
  GCAAGTATTCAAACGCAAATAGAAATATTAAAATCACAAAATAGTTTCCCTAAAAATCTT
  TCATATCTTAAAAGTTATACGGACCCCAAAACAGGTACGACTACAAGCGCCTTTTTAAAT
  AAAGACACTGGCAAAGTTACTTTAGGTATGACAGGTACTAATGTACACAAAGACGCAATA
  TTAAAACAAACATTTGGTGTTCCTTCTTATCAAGGATATATAGATGCGAGTGAAACGCTA
  AAGGATATTGGGGCCGATGTCAATATTGGCCTTCATTCCGTCACAGATAAAGATCCACAT
  TATAAAAATACCCAAGACTTTATCAAAAATATCAAAAAAGACTATGATATTGATATTATT
  ACCGGACATTCGCTGGGCGGTAGAGATGCGATGATTTTAGGTATGAGTAATGATATTAAA
  CATATCGTTGTGTATAATCCAGCTCCATTAGCGATCAAAGATGTGAGCGGCCTTTATGCC
  GATCAAGAAGAACTTAAAAAATTGATTGAAAAGTACGATGGTCACATTGTAAGATTTGTG
  TCTGATGAAGACGAATTAGATGCAGGTGTCCGCAATCATTTATATGAAACTGCTGGAGAA
  AAAATAGTACTTAAAAATGGAGAAGGCCATGCAATGAGTGGTATTTTAATGAGCAGAACA
  CAGGCTATAATCTTAGCTGAATTAAACAAAGTTAAAGGCTACCAAGACGAAAATAATAAA
  GCATTAAAATCCGTTCGTAAACAAACGAGGCATAGATTACATAAAGTAGAGACGTTAAGA
  GCGAATTGGATTCAAACAACGGGTGGATCACTCTCTTCCTCCCAACAACAATTATTAGAA
  GCTTTAACAGCACTAACCATTGCCGAAGGCTTAAATCAATTAGTGAATGAAGAAAGCCAA
  CATTTGAAAAAAATGTATCACGCGATGGCACATAAATTTGGAGACAACTGGAAAAAAGCG
  CAAGAAGTTGGAAATGAAATTGGTGAAAAATTAACCTCTGAAGAGGTTATAGATGAATTA
  AGAAAAGGTGGCGCGTATGAAAGTAAACTTGAAACAGATCCCAAAAGAAAAATTGATGAT
  AAGATAAAGAAATTAAATGATGTTTATAAAAATTGTAATGGCTATATCGCAAAAATTAAA
  CAGAGTATCGAAGCAATTGTTTCTAATGACCAAATGTTAGCGAGCCAGATTGATGGGATG
  ATGTAA
  60
  120
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  480
  540
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  840
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  960
  1020
  1080
  1140
  1200
  1260
  1320
  1326
  

⊟Protein

⊟General

• locus tag: SA_RS13045 [old locus tag: SA2272 ]
• symbol: SA_RS13045
• description: membrane protein
• length: 441
• theoretical pI: 6.71552
• theoretical MW: 49186.1
• GRAVY: -0.622903

⊟Function

• TIGRFAM:
  phasin (TIGR01985; HMM-score: 12.7)
• TheSEED: see SA2272
• PFAM:
  no clan defined DUF2959; Protein of unknown function (DUF2959) (PF11172; HMM-score: 15.3)
  AB_hydrolase (CL0028) Lipase_3; Lipase (class 3) (PF01764; HMM-score: 15.2)
  no clan defined Phasin_2; Phasin protein (PF09361; HMM-score: 14.5)
  AB_hydrolase (CL0028) UPF0227; Uncharacterised protein family (UPF0227) (PF05728; HMM-score: 14.2)
  no clan defined DUF4413; Domain of unknown function (DUF4413) (PF14372; HMM-score: 12.7)
  BRCT-like (CL0459) BRCT_2; BRCT domain, a BRCA1 C-terminus domain (PF16589; HMM-score: 12.3)
  and 2 more
  no clan defined DUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 11.6)
  Gp-FAR-1; Nematode fatty acid retinoid binding protein (Gp-FAR-1) (PF05823; HMM-score: 10.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025314
  • TAT(Tat/SPI): 0.002743
  • LIPO(Sec/SPII): 0.005311
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446966999 NCBI
• RefSeq: WP_001044255 NCBI
• UniProt: see SA2272

⊟Protein sequence

• MNKTKYQINSDNIKSNSEETSAISSISYEIENANNNDLNNASIQTQIEILKSQNSFPKNLSYLKSYTDPKTGTTTSAFLNKDTGKVTLGMTGTNVHKDAILKQTFGVPSYQGYIDASETLKDIGADVNIGLHSVTDKDPHYKNTQDFIKNIKKDYDIDIITGHSLGGRDAMILGMSNDIKHIVVYNPAPLAIKDVSGLYADQEELKKLIEKYDGHIVRFVSDEDELDAGVRNHLYETAGEKIVLKNGEGHAMSGILMSRTQAIILAELNKVKGYQDENNKALKSVRKQTRHRLHKVETLRANWIQTTGGSLSSSQQQLLEALTALTIAEGLNQLVNEESQHLKKMYHAMAHKFGDNWKKAQEVGNEIGEKLTSEEVIDELRKGGAYESKLETDPKRKIDDKIKKLNDVYKNCNGYIAKIKQSIEAIVSNDQMLASQIDGMM

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications