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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1631 [new locus tag: NWMN_RS09165 ]
  • pan locus tag?: SAUPAN004405000
  • symbol: NWMN_1631
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1631 [new locus tag: NWMN_RS09165 ]
  • symbol: NWMN_1631
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1816466..1817983
  • length: 1518
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACGCAGCAACAAAATGATAAAAGAACATTAAAAAATAAACACACTTATCAAAATGAA
    CCACTACCAAACCGTAAAGATTTTGTTGTTAGTTTTATAACTGGCGCGCTTGTTGGTTCA
    GCTTTAGGTTTGTATTTTAAAAATAAAGTTTATCAAAAAGCAGATGATTTAAAAGTCAAA
    GAACAAGAACTGTCGCAAAAGTTTGAAGAAAGAAAAACGCAACTTGAAGAAACGGTTGCC
    TATACAAAAGAACGTGTTGAAGGATTTTTAAACAAATCTAAAAATGAGCAAGCGGCATTA
    AAGGCACAACAAGCAGCGATAAAAGAAGAAGCAAGTGCAAATAATTTAAGTGATACATCA
    CAAGAAGCACAAGAGATTCAAGAAGCTAAAAGAGAAGCACAAGCAGAAGCGGATAAAAGT
    GTGGCTGTATCAAATAAAGAATCAAAAGCAGTGGCATTGAAAGCACAACAAGCAGCGATA
    AAAGAAGAAGCAAGTGCAAATAATTTGAGTGATACATCACAAGAGGCACAAGAGATTCAA
    GAAGCTAAAAAAGAAGCACAAGCAGAAACAGATAAAAGTGCAGCTGTATCAAATGAAGAA
    CCAAAAGCAGTGGCATTGAAAGCACAACAAGCAGCGATAAAAGAAGAAGCAAGTGCAAAT
    AATTTAAGTGATACATCACAAGAGGCACAAGAGGTTCAAGAAGCTAAAAAAGAAGCACAA
    GCAGAAACAGATAAAAGTGCAGCTGTATCAAATGAAGAACCAAAAGCAGTGGCATTGAAA
    GCACAACAAGCAGCGATAAAAGAAGAAGCAAGTGCAAATAATTTAAGTGATATATCACAA
    GAGGCACAAGAGGTTCAAGAAGCTAAAAAAGAAGCACAAGCAGAGAAAGACAGTGACACA
    TTAACTAAAGATGCAAGTGCAGCAAAGGTAGAAGTATCAAAACCAGAGTCACAAGCTGAA
    AGATTAGCAAACGCTGCAAAACAGAAGCAAGCTAAATTAACACCAGGTTCAAAAGAGAGT
    CAATTAACTGAAGCGTTATTTGCAGAAAAACCAGTTGCTAAAAATGACTTGAAAGAAATT
    CCTCAATTAGTTACTAAAAAGAATGATGTATCAGAGACAGAGACGGTTAATATAGATAAT
    AAAGACACTGTTAAACAAAAAGAAGCTAAATTTGAAAATGGTGTTATTACACGTAAAGCT
    GATGAAAAAACAACTAATAATACAGCTGTTGACAAGAAATCAGGTAAACAATCTAAAAAA
    ACAACACCTTCAAATAAACGAAATGCATCAAAAGCATCTACAAATAAAACTTCAGGTCAG
    AAAAAGCAACATAATAAGAAATCATCACAAGGTGCAAAGAAACAAAGTAGTTCAAGTAAG
    TCAACTCAAAAGAATAATCAAACTAGTAATAAGAATTCAAAAACAACAAATGCTAAATCA
    TCCAATGCATCAAAAACGCCAAATGCTAAAGTTGAGAAAGCTAAAAGTAAAATAGAGAAA
    CGTACATTCAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1518

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1631 [new locus tag: NWMN_RS09165 ]
  • symbol: NWMN_1631
  • description: hypothetical protein
  • length: 505
  • theoretical pI: 9.87695
  • theoretical MW: 55090.3
  • GRAVY: -1.18812

⊟Function[edit | edit source]

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.11
    • Cellwall Score: 9.25
    • Extracellular Score: 0.63
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014086
    • TAT(Tat/SPI): 0.022843
    • LIPO(Sec/SPII): 0.00329
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGLYFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKAQQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAIKEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEASANNLSDTSQEAQEVQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEASANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTFND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    NWMN_2007(atpD)F0F1 ATP synthase subunit beta  [1] (data from MRSA252)
    NWMN_0745(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    NWMN_0473(ftsH)ATP-dependent metalloprotease FtsH  [1] (data from MRSA252)
    NWMN_1096(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    NWMN_2017(glyA)serine hydroxymethyltransferase  [1] (data from MRSA252)
    NWMN_1574(infC)translation initiation factor IF-3  [1] (data from MRSA252)
    NWMN_1268(parC)DNA topoisomerase IV subunit A  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2152(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    NWMN_2151(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    NWMN_2140(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_0499(rplK)50S ribosomal protein L11  [1] (data from MRSA252)
    NWMN_0502(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_2145(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    NWMN_2125(rplQ)50S ribosomal protein L17  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1549(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_2024(rpmE2)50S ribosomal protein L31 type B  [1] (data from MRSA252)
    NWMN_1613(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0357(rpsF)30S ribosomal protein S6  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_2127(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    NWMN_0507(rpsL)30S ribosomal protein S12  [1] (data from MRSA252)
    NWMN_2143(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    NWMN_2148(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    NWMN_1539(secDF)bifunctional preprotein translocase subunit SecD/SecF  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_02495'-nucleotidase, lipoprotein e(P4) family protein  [1] (data from MRSA252)
    NWMN_0632hypothetical protein  [1] (data from MRSA252)
    NWMN_1099hypothetical protein  [1] (data from MRSA252)
    NWMN_1356hypothetical protein  [1] (data from MRSA252)
    NWMN_1382DNA-binding protein HU  [1] (data from MRSA252)
    NWMN_1497hypothetical protein  [1] (data from MRSA252)
    NWMN_1600universal stress protein family protein  [1] (data from MRSA252)
    NWMN_1621hypothetical protein  [1] (data from MRSA252)
    NWMN_1634FtsK/SpoIIIE (DNA translocase stage III) family protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [2] [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  3. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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