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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1330 [new locus tag: NWMN_RS07490 ]
  • pan locus tag?: SAUPAN003843000
  • symbol: murG
  • pan gene symbol?: murG
  • synonym:
  • product: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1330 [new locus tag: NWMN_RS07490 ]
  • symbol: murG
  • product: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1463666..1464736
  • length: 1071
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACGAAAATCGCATTTACCGGAGGGGGAACAGTTGGACACGTATCAGTAAATTTAAGT
    TTAATTCCAACTGCATTATCACAAGGTTATGAAGCGCTTTATATTGGTTCTAAAAATGGT
    ATTGAAAGAGAAATGATTGAATCACAACTACCAGAAATTAAGTATTATCCTATTTCGAGT
    GGTAAATTAAGAAGATATATTTCTTTAGAAAATGCCAAAGACGTATTTAAAGTATTGAAA
    GGTATTCTTGATGCTCGTAAAGTTTTGAAAAAAGAAAAACCTGATCTATTATTTTCAAAA
    GGTGGATTTGTATCTGTGCCTGTTGTTATTGCAGCCAAATCATTAAATATACCAACTATT
    ATTCATGAATCTGACTTAACACCAGGATTAGCGAATAAGATAGCACTTAAATTTGCCAAG
    AAAATATATACAACATTTGAAGAAACGCTAAACTACTTACCTAAAGAGAAAGCTGATTTT
    ATTGGAGCAACAATTCGAGAAGATTTAAAAAATGGTAATGCACATAATGGTTATCAATTA
    ACAGGCTTTAATGAAAATAAAAAAGTTTTACTCGTTATGGGTGGAAGCTTAGGAAGTAAA
    AAATTAAATAGCATTATTCGCGAAAACTTAGATGCATTATTACAACAATATCAAGTGATA
    CATTTAACTGGTAAAGGATTAAAAGATGCTCAAGTTAAAAAATCAGGATATATACAATAT
    GAATTTGTTAAAGAGGATTTAACAGATTTATTAGCAATTACGGATACAGTAATAAGTAGA
    GCTGGATCAAATGCGATTTATGAGTTCTTAACATTACGTATACCAATGTTATTAGTACCA
    TTAGGTTTAGATCAATCCCGAGGCGACCAAATTGACAATGCAAATCATTTTGCTGATAAA
    GGATATGCTAAAGCGATTGATGAAGAACAATTAACAGCACAAATTTTATTACAAGAACTA
    AATGAAATGGAACAGGAAAGAACTCGAATTATCAATAATATGAAATCGTATGAACAAAGT
    TATACGAAAGAAGCTTTATTTGATAAGATGATTAAAGACGCATTGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1071

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1330 [new locus tag: NWMN_RS07490 ]
  • symbol: MurG
  • description: undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
  • length: 356
  • theoretical pI: 9.13983
  • theoretical MW: 39696.5
  • GRAVY: -0.227528

Function[edit source | edit]

  • reaction:
    EC 2.4.1.227?  ExPASy
    Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferaseUDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanundecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 244.5)
    Cellular processesCellular processesToxin production and resistanceglycosyltransferase, MGT family (TIGR01426; EC 2.4.1.-; HMM-score: 41.5)
    Hypothetical proteinsConservedconserved hypothetical protein (TIGR00661; HMM-score: 31.6)
  • TheSEED:  
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227) 
    Cell Division Cluster UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227) 
  • PFAM:
    GT-B (CL0113) Glyco_transf_28; Glycosyltransferase family 28 N-terminal domain (PF03033; HMM-score: 130.6)
    Glyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 95)
    UDPGT; UDP-glucoronosyl and UDP-glucosyl transferase (PF00201; HMM-score: 29.7)
    Glyco_trans_1_3; Glycosyl transferase family 1 (PF13528; HMM-score: 18)
    Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 15.3)
    no clan definedOberon_cc; Coiled-coil region of Oberon (PF16312; HMM-score: 14.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_1580(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    NWMN_0176(ldh1)L-lactate dehydrogenase  [1] (data from MRSA252)
    NWMN_2004(murA)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [1] (data from MRSA252)
    NWMN_2446(mvaS)3-hydroxy-3-methylglutaryl coenzyme A synthase  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_2016(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    NWMN_0620hypothetical protein  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_0881enoyl-(acyl carrier protein) reductase  [1] (data from MRSA252)
    NWMN_1604universal stress protein family protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.192
    • Ymax_pos: 28
    • Cmax: 0.159
    • Cmax_pos: 28
    • Smax: 0.368
    • Smax_pos: 24
    • Smean: 0.22
    • D: 0.203
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIEREMIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGGFVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADFIGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQVIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPMLLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNMKSYEQSYTKEALFDKMIKDALN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]