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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1519 [new locus tag: NWMN_RS08540 ]
  • pan locus tag?: SAUPAN004215000
  • symbol: alaS
  • pan gene symbol?: alaS
  • synonym:
  • product: alanyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1519 [new locus tag: NWMN_RS08540 ]
  • symbol: alaS
  • product: alanyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1682058..1684688
  • length: 2631
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAAAAGTTAAAAGCGAGTGAAATTAGACAAAAATATCTAGATTTCTTTGTTGAAAAA
    GGACATATGGTTGAACCTTCTGCACCATTAGTGCCAATTGATGATGATACATTATTATGG
    ATTAATTCAGGTGTAGCAACATTAAAGAAATATTTTGATGGACGTGAAACACCTAAAAAG
    CCAAGAATTGTAAACTCTCAAAAAGCTATTCGTACAAATGATATTGAAAATGTTGGTTTC
    ACAGCGCGTCACCATACATTCTTTGAAATGTTAGGTAACTTCTCAATTGGTGATTATTTT
    AAACAAGAAGCGATTGAATTTGCTTGGGAATTTTTAACGAGTGATAAATGGATGGGTATG
    GAGCCAGATAAATTGTACGTTACGATTCATCCGGAAGATATGGAAGCATACAACATTTGG
    CATAAAGATATTGGGCTTGAAGAAAGTCGTATTATTCGCATTGAAGGTAACTTCTGGGAT
    ATTGGTGAAGGGCCTTCAGGACCGAACACTGAGATTTTCTATGATCGCGGAGAAGCATAT
    GGACAAGACGATCCGGCAGAAGAAATGTATCCAGGTGGAGAAAATGAACGCTATCTTGAA
    GTATGGAACTTAGTATTTAGTGAATTCAATCATAATAAAGATCATAGTTACACACCATTA
    CCTAATAAAAATATTGATACTGGCATGGGGCTTGAGCGTATGGCCTCAGTTTCTCAAAAT
    GTACGTACTAACTATGAAACAGATTTATTTATGCCTATAATGAATGAAATCGAAAAAGTA
    TCAGGTAAACAATATTTAGTAAACAACGAACAAGATGTGGCATTTAAAGTAATTGCTGAC
    CACATTCGTACGATTGCATTTGCAATTTCTGATGGTGCATTACCTGCCAATGAAGGTAGA
    GGGTATGTATTACGTCGATTGTTACGTCGTGCCGTTCGTTTTAGTCAAACGTTAGGAATC
    AATGAGCCATTTATGTACAAACTTGTTGATATTGTTGCAGACATTATGGAACCATATTAT
    CCAAATGTTAAGGAAAAAGCAGATTTCATTAAGCGTGTTATAAAGTCTGAAGAAGAACGA
    TTCCATGAAACATTAGAAGATGGTTTAGCGATTTTAAATGAATTAATTAAAAAAGCTAAA
    GCGACAACAAATGAAATTAATGGGAAAGATGCATTTAAATTGTATGATACGTATGGGTTC
    CCAATTGAATTAACTGAAGAAATAGCAGTGCAAGCAGGATTGAAAGTTGATATGACAACA
    TTCGAGTCAGAAATGCAACAACAACGTGATCGTGCACGTCAAGCACGTCAAAATTCTCAA
    TCAATGCAAGTTCAAAGTGAAGTATTGAAAAATATTACATCTGCAAGTACTTTTGTTGGT
    TATGATACTGCGACAGCTCAAACAACACTAACACACTTGATATACAATGGTGAAGAAGTT
    TCACAAGTTGAAGCGGGTGAAACAGTATACTTCATGTTAACGGAAACACCATTTTATGCA
    ATCAGTGGTGGACAAGTTGCGGATACAGGTATTGTTTATAATGACAATTTTGAAATTGCT
    GTTAGTGAAGTAACCAAAGCACCAAATGGTCAAAACTTGCATAAAGGAGTAGTACAATTT
    GGCCAAGTAAATGTTGGCGCTACAGTGTCTGCTGAAGTGAACCAAAATGATCGACGTGAC
    ATTCAAAAGAACCATAGTGCAACACATTTATTACATGCAGCGTTGAAATCAGTACTGGGT
    GATCATGTTAACCAAGCTGGTTCACTAGTAGAAGCAGATCGTTTACGTTTTGATTTCTCT
    CATTTTGGTCCAATGACTAATGATGAAATTGATCAAGTTGAACGCTTAGTAAATGAAGAA
    ATTTGGAAAGGTATTGACGTTAACATTCAAGAAATGGATATTGCTTCAGCTAAAGAAATG
    GGCGCAATGGCATTATTCGGTGAAAAATATGGTGATGTTGTGCGTGTAGTAAATATGGCA
    CCATTTTCAATTGAATTATGTGGTGGTATTCATGTCCGCAATACTTCTGAAATTGGCTTA
    TTCAAAATAGTAAGTGAGTCAGGTACAGGAGCTGGTGTGCGTCGTATTGAAGCATTAACA
    GGTAAAGCAGCTTTCTTATATTTAGAAGATATTCAAGAGAAATTTAATACGATGAAATCA
    CAGCTGAAAGTGAAATCTGATGATCAAGTAGTCGATAAGTTAACACAATTACAAGATGAA
    GAAAAAGCATTATTAAAACAATTAGAGCAACGTGACAAAGAAATCACATCACTTAAAATG
    GGTAATATTGAAGATCAAGTTGAAGAAATCAATGGCTATAAAGTATTGGTTACTGAAGTG
    GATGTACCAAATGCGAAAGCAATTCGCTCGACAATGGACGATTTTAAATCTAAACTACAA
    GATACAATTATCATTCTTGCAAGTAATGTTGATGATAAAGTATCGATGGTTGCAACTGTC
    CCTAAATCTTTAACAAATAACGTTAAAGCCGGTGATCTTATCAAACAAATGGCACCAATC
    GTTGGTGGTAAAGGTGGCGGTCGTCCAGATATGGCTCAAGGTGGCGGTACACAACCTGAA
    AATATCTCAAAATCATTAAGCTTTATTAAAGATTACATTAAAAATCTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2631

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1519 [new locus tag: NWMN_RS08540 ]
  • symbol: AlaS
  • description: alanyl-tRNA synthetase
  • length: 876
  • theoretical pI: 4.75077
  • theoretical MW: 98519.6
  • GRAVY: -0.418379

Function[edit source | edit]

  • reaction:
    EC 6.1.1.7?  ExPASy
    Alanine--tRNA ligaseATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationalanine--tRNA ligase (TIGR00344; EC 6.1.1.7; HMM-score: 1065.1)
    alanine--tRNA ligase (TIGR03683; EC 6.1.1.7; HMM-score: 421.9)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesclan AA aspartic protease, TIGR02281 family (TIGR02281; EC 3.4.23.-; HMM-score: 12.1)
  • TheSEED:  
    Protein MetabolismProtein biosynthesistRNA aminoacylation, Ala Alanyl-tRNA synthetase (EC 6.1.1.7) 
    CBSS-257314.1.peg.488 Alanyl-tRNA synthetase (EC 6.1.1.7) 
    CBSS-281090.3.peg.464 Alanyl-tRNA synthetase (EC 6.1.1.7) 
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2c; tRNA synthetases class II (A) (PF01411; HMM-score: 696.1)
    no clan definedDHHA1; DHHA1 domain (PF02272; HMM-score: 107.2)
    Peptidase_ME (CL0094) tRNA_SAD; Threonyl and Alanyl tRNA synthetase second additional domain (PF07973; HMM-score: 65)
    no clan definedSwm2; Nucleolar protein Swm2 (PF17083; HMM-score: 14.8)
    FAM76; FAM76 protein (PF16046; HMM-score: 9.7)
    CCDC24; Coiled-coil domain-containing protein 24 family (PF15669; HMM-score: 8.9)
    IncA; IncA protein (PF04156; HMM-score: 6.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_2040(pdp)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_0502(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_1323hypothetical protein  [1] (data from MRSA252)
    NWMN_1604universal stress protein family protein  [1] (data from MRSA252)
    NWMN_2504malate:quinone oxidoreductase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.125
    • Ymax_pos: 11
    • Cmax: 0.106
    • Cmax_pos: 7
    • Smax: 0.183
    • Smax_pos: 1
    • Smean: 0.15
    • D: 0.135
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWDIGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSKLQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]