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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06515 [old locus tag: NWMN_1155 ]
  • pan locus tag?: SAUPAN003537000
  • symbol: NWMN_RS06515
  • pan gene symbol?: sucC
  • synonym:
  • product: succinyl-CoA ligase subunit beta

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS06515 [old locus tag: NWMN_1155 ]
  • symbol: NWMN_RS06515
  • product: succinyl-CoA ligase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 1266843..1268009
  • length: 1167
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1266843..1268009) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATATCCACGAGTATCAAGGTAAAGAAATATTTCGTTCAATGGGCGTTGCAGTTCCA
    GAAGGACGAGTAGCATTTACTGCTGAAGAAGCGGTGGAGAAAGCAAAAGAATTAAATTCT
    GATGTTTATGTTGTAAAAGCACAAATTCATGCTGGAGGTAGAGGTAAAGCAGGCGGAGTA
    AAAATTGCTAAATCTTTATCTGAGGTAGAAACATATGCAAAAGAATTATTAGGGAAAACT
    TTGGTGACACATCAAACTGGTCCAGAAGGTAAAGAAATTAAGCGTTTATATATCGAAGAA
    GGTTGTGCTATTCAAAAAGAATATTACGTTGGATTCGTTATTGATCGTGCGACTGACCAA
    GTAACATTGATGGCGTCTGAAGAAGGGGGCACTGAGATTGAAGAAGTTGCTGCGAAGACT
    CCTGAAAAGATCTTCAAAGAAACTATCGATCCAGTAATCGGACTTTCACCATTCCAAGCA
    AGACGAATTGCGTTTAATATTAATATTCCTAAAGAATCTGTTAACAAAGCTGCTAAATTC
    TTATTAGCACTTTATAATGTATTCATTGAAAAAGATTGTTCAATCGTAGAAATCAACCCA
    TTAGTTACAACAGCTGATGGTGATGTATTGGCATTAGATGCTAAAATTAATTTTGATGAT
    AATGCATTATTCAGACATAAAGATGTTGTAGAATTACGTGATTTAGAAGAAGAAGATCCG
    AAAGAGATTGAAGCGTCTAAACATGATTTATCATACATTGCATTAGATGGTGACATCGGA
    TGTATGGTTAATGGTGCAGGTTTAGCCATGGCAACAATGGATACGATTAATCATTTCGGT
    GGAAACCCAGCCAATTTCTTAGATGCAGGCGGAAGCGCTACTAGAGAAAAAGTAACTGAA
    GCATTTAAAATCATTTTAGGTGATGAAAATGTTAAAGGTATTTTTGTAAACATTTTCGGT
    GGCATTATGAAATGTGATGTTATCGCAGAAGGTATCGTTGAAGCTGTAAAAGAAGTAGAT
    TTAACTTTACCACTAGTTGTACGCCTAGAAGGTACAAATGTTGAGTTAGGTAAAAAAATC
    TTAAAAGACTCAGGATTAGCAATTGAACCAGCAGCAACAATGGCTGAAGGTGCACAAAAA
    ATTGTTAAACTAGTCAAAGAAGCATAA
    60
    120
    180
    240
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    360
    420
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    540
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    720
    780
    840
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    960
    1020
    1080
    1140
    1167

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS06515 [old locus tag: NWMN_1155 ]
  • symbol: NWMN_RS06515
  • description: succinyl-CoA ligase subunit beta
  • length: 388
  • theoretical pI: 4.63116
  • theoretical MW: 42056
  • GRAVY: -0.0510309

Function[edit source | edit]

  • reaction:
    EC 6.2.1.5?  ExPASy
    Succinate--CoA ligase (ADP-forming)ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cyclesuccinate-CoA ligase, beta subunit (TIGR01016; EC 6.2.1.-; HMM-score: 545.1)
    acetyl coenzyme A synthetase (ADP forming), alpha domain (TIGR02717; EC 6.2.1.13; HMM-score: 21.8)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationputative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 17.4)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 15.3)
  • TheSEED:
  • PFAM:
    ATP-grasp (CL0179) ATP-grasp_2; ATP-grasp domain (PF08442; HMM-score: 279.1)
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 84.9)
    ATP-grasp (CL0179) ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 40.2)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 17.4)
    Succ_CoA_synth (CL0506) Citrate_bind; ATP citrate lyase citrate-binding (PF16114; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:
  • protein partners:
    NWMN_RS03640LysR family transcriptional regulator  [1] (data from MRSA252)
    NWMN_RS04730hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 31
    • Cmax: 0.12
    • Cmax_pos: 31
    • Smax: 0.342
    • Smax_pos: 25
    • Smean: 0.145
    • D: 0.144
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446943545 NCBI
  • RefSeq: WP_001020801 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYVVKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGCAIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQARRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINFDDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTINHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVEAVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS06515 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]