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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1521 [new locus tag: SA_RS08565 ]
  • pan locus tag?: SAUPAN004325000
  • symbol: pfkA
  • pan gene symbol?: pfkA
  • synonym:
  • product: 6-phosphofructokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1521 [new locus tag: SA_RS08565 ]
  • symbol: pfkA
  • product: 6-phosphofructokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1736036..1736959
  • length: 924
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAATGCTGCCGTAAGAGCAGTTGTTCGTACAGCAATTTACAATGAAATTGAAGTTTAT
    GGTGTGTATCATGGTTACCAAGGATTGTTAAATGATGATATTCATAAACTTGAATTAGGA
    TCAGTTGGGGATACGATTCAGCGTGGAGGTACATTCTTGTATTCAGCAAGATGTCCAGAG
    TTTAAGGAGCAAGAAGTACGTAAAGTTGCAATCGAAAACTTACGTAAAAGAGGGATTGAG
    GGCCTTGTAGTTATTGGTGGTGACGGTAGTTATCGCGGTGCACAACGCATCAGTGAGGAA
    TGCAAAGAAATTCAAACTATCGGTATTCCTGGTACGATTGACAATGATATCAATGGTACT
    GATTTTACAATTGGATTTGACACAGCTTTAAATACGATTATTGGCTTAGTCGACAAAATT
    AGAGATACTGCGTCAAGTCACGCACGAACATTTATCATTGAAGCAATGGGCCGTGATTGT
    GGAGATCTAGCATTATGGGCTGGATTATCAGTTGGTGCTGAGACAATTGTAGTTCCAGAA
    GTGAAAACAGATATTAAAGAAATAGCTGATAAAATTGAACAAGGTATTAAACGTGGTAAG
    AAACACTCAATCGTTCTTGTAGCAGAAGGTTGCATGACTGCGCAAGATTGTCAAAAAGAA
    TTATCACAATACATCAATGTTGATAATAGAGTGTCTGTGTTAGGTCACGTTCAACGTGGT
    GGTAGCCCAACAGGTGCGGATAGAGTTTTAGCATCACGTTTAGGTGGATATGCGGTAGAC
    TTATTAATGCAAGGTGAAACAGCTAAGGGTGTTGGAATTAAGAACAATAAAATTGTAGCA
    ACATCTTTTGATGAAATTTTTGATGGTAAAGATCATAAATTTGATTATAGTCTATATGAA
    CTTGCTAACAAGTTATCTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    924

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1521 [new locus tag: SA_RS08565 ]
  • symbol: PfkA
  • description: 6-phosphofructokinase
  • length: 307
  • theoretical pI: 5.45417
  • theoretical MW: 33396.7
  • GRAVY: -0.167752

Function[edit source | edit]

  • reaction:
    EC 2.7.1.11?  ExPASy
    6-phosphofructokinaseATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesis6-phosphofructokinase (TIGR02482; EC 2.7.1.11; HMM-score: 396.5)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphofructokinase (TIGR02483; HMM-score: 273.9)
    6-phosphofructokinase (TIGR02478; EC 2.7.1.11; HMM-score: 263.9)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisdiphosphate--fructose-6-phosphate 1-phosphotransferase (TIGR02477; EC 2.7.1.90; HMM-score: 76.9)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismGlycolysis and Gluconeogenesis 6-phosphofructokinase (EC 2.7.1.11) 
    MonosaccharidesD-Tagatose and Galactitol Utilization 6-phosphofructokinase (EC 2.7.1.11) 
  • PFAM:
    PFK (CL0240) PFK; Phosphofructokinase (PF00365; HMM-score: 338.6)
    NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 13)
    DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:
    SA1984(asp23)alkaline shock protein 23  [2] (data from MRSA252)
    SA0505(fus)elongation factor G  [2] (data from MRSA252)
    SA2400(mqo2)malate:quinone oxidoreductase  [2] (data from MRSA252)
    SA1244(odhB)dihydrolipoamide succinyltransferase  [2] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [2] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [2] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [2] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [2] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [2] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [2] (data from MRSA252)
    SA2040(rplP)50S ribosomal protein L16  [2] (data from MRSA252)
    SA1084(rplS)50S ribosomal protein L19  [2] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA0802hypothetical protein  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 70
    • Cmax: 0.116
    • Cmax_pos: 22
    • Smax: 0.135
    • Smax_pos: 1
    • Smean: 0.082
    • D: 0.094
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol. Microbiol.: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] (P p)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)