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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1593 [new locus tag: NWMN_RS08935 ]
  • pan locus tag?: SAUPAN004325000
  • symbol: pfk
  • pan gene symbol?: pfkA
  • synonym:
  • product: 6-phosphofructokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1593 [new locus tag: NWMN_RS08935 ]
  • symbol: pfk
  • product: 6-phosphofructokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1766545..1767513
  • length: 969
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGAAAATTGCAGTTTTAACTAGTGGTGGAGATTCACCTGGAATGAATGCTGCCGTA
    AGAGCAGTTGTTCGTACAGCAATTTACAATGAAATTGAAGTTTATGGTGTGTATCATGGT
    TACCAAGGATTGTTAAATGATGATATTCATAAACTTGAATTAGGATCAGTTGGGGATACG
    ATTCAGCGTGGAGGTACATTCTTGTATTCAGCAAGATGTCCAGAGTTTAAGGAGCAAGAA
    GTACGTAAAGTTGCAATCGAAAACTTACGTAAAAGAGGGATTGAGGGCCTTGTAGTTATT
    GGTGGTGACGGTAGTTATCGCGGTGCACAACGCATCAGTGAGGAATGTAAAGAAATTCAA
    ACTATCGGTATTCCTGGTACGATTGACAATGATATCAATGGTACTGATTTTACAATTGGA
    TTTGACACAGCATTAAATACGATTATTGGCTTAGTCGACAAAATTAGAGATACTGCGTCA
    AGTCACGCACGAACATTTATCATTGAAGCAATGGGCCGTGATTGTGGAGATCTAGCATTA
    TGGGCTGGATTATCAGTTGGTGCTGAGACAATTGTAGTTCCAGAAGTGAAAACAGATATT
    AAAGAAATAGCTGATAAAATTGAACAAGGTATTAAACGTGGTAAGAAACACTCAATCGTT
    CTTGTAGCAGAAGGTTGTATGACTGCGCAAGATTGTCAAAAAGAATTATCACAATACATC
    AATGTTGATAATAGAGTGTCTGTGTTAGGTCACGTTCAACGTGGTGGTAGCCCAACAGGT
    GCGGATAGAGTTTTAGCATCACGTTTAGGTGGATATGCGGTAGACTTATTAATGCAAGGT
    GAAACAGCTAAGGGTGTTGGAATTAAGAACAATAAAATTGTAGCAACATCTTTTGATGAA
    ATTTTTGATGGTAAAGATCATAAATTTGATTATAGTCTATATGAACTTGCTAACAAGTTA
    TCTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1593 [new locus tag: NWMN_RS08935 ]
  • symbol: Pfk
  • description: 6-phosphofructokinase
  • length: 322
  • theoretical pI: 5.71393
  • theoretical MW: 34839.4
  • GRAVY: -0.160559

Function[edit source | edit]

  • reaction:
    EC 2.7.1.11?  ExPASy
    6-phosphofructokinaseATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesis6-phosphofructokinase (TIGR02482; EC 2.7.1.11; HMM-score: 428)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphofructokinase (TIGR02483; HMM-score: 301.2)
    6-phosphofructokinase (TIGR02478; EC 2.7.1.11; HMM-score: 295.8)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisdiphosphate--fructose-6-phosphate 1-phosphotransferase (TIGR02477; EC 2.7.1.90; HMM-score: 82.2)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismGlycolysis and Gluconeogenesis 6-phosphofructokinase (EC 2.7.1.11) 
    MonosaccharidesD-Tagatose and Galactitol Utilization 6-phosphofructokinase (EC 2.7.1.11) 
  • PFAM:
    PFK (CL0240) PFK; Phosphofructokinase (PF00365; HMM-score: 367.1)
    NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 13.1)
    DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0960(phdB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2145(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)
    NWMN_2504malate:quinone oxidoreductase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 22
    • Cmax: 0.134
    • Cmax_pos: 22
    • Smax: 0.376
    • Smax_pos: 18
    • Smean: 0.219
    • D: 0.191
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15

Relevant publications[edit source | edit]