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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0765 [new locus tag: SACOL_RS03930 ]
  • pan locus tag?: SAUPAN002590000
  • symbol: saeS
  • pan gene symbol?: saeS
  • synonym:
  • product: sensor histidine kinase SaeS

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0765 [new locus tag: SACOL_RS03930 ]
  • symbol: saeS
  • product: sensor histidine kinase SaeS
  • replicon: chromosome
  • strand: -
  • coordinates: 787731..788786
  • length: 1056
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTGTTATCAATTAGAAGTCAAATCATTATTGGCGTCGTATCGAGTATACTATTAACT
    TCAACTATTTTAGCAATTGCATATATTTTAATGTGGTTTAACGGCCATATGACACTAACT
    TTGACCTTAACGACAATAATTACAAGCTGTTTAACCTTATTAATATGTAGTATTTTTATT
    AATCCACTTATACAAAAAATTAAGCAGTTTAATATAAAAACTAAGCAATTTGCTAACGGA
    AATTACGCAAGCAATGATAAAACGTTTAATTCACCAAAAGAAATTTATGAATTAAATCAA
    TCTTTTAATAAAATGGCTTCTGAAATTACGCAACAAATGAATCAAATTAAATCCGAACAA
    CAAGAAAAAACAGAACTGATTCAAAACTTAGCCCATGATTTAAAAACACCTTTAGCAAGC
    ATTATTTCATATTCTGAAGGACTACGTGATGGTATAATCACTAAGGATCATGAGATTAAA
    GAGTCATACGACATATTAATTAAACAAGCAAACAGATTATCAACATTATTTGATGATATG
    ACTCATATTATCACTTTAAATACAGGTAAAACATATCCCCCAGAATTAATACAACTAGAC
    CAATTACTTGTATCAATATTGCAACCATATGAGCAACGTATCAAACATGAAAACCGCACA
    TTAGAAGTGAATTTCTGTAACGAAATTGATGCATTTTATCAATATCGAACGCCACTTGAG
    CGTATTTTAACAAACTTACTTGATAATGCGCTAAAATTTTCAAATGTTGGTAGTCGCATT
    GATATTAATATTAGTGAAAACGAAGATCAAGATACTATCGACATTGCTATTAGCGATGAA
    GGTATTGGCATTATACCAGAACTACAAGAACGTATATTCGAACGTACATTCAGAGTAGAA
    AACTCTCGTAATACAAAAACGGGTGGTTCTGGATTAGGCTTATATATAGCTAATGAACTC
    GCGCAACAAAATAACGCAAAAATCAGTGTAAGCAGTGATATAGATGTAGGAACTACGATG
    ACTGTAACATTACACAAATTAGACATTACGTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0765 [new locus tag: SACOL_RS03930 ]
  • symbol: SaeS
  • description: sensor histidine kinase SaeS
  • length: 351
  • theoretical pI: 4.97145
  • theoretical MW: 39741.2
  • GRAVY: -0.132764

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 140.7)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 106.6)
    Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 85.9)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 64)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 50.6)
    MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
    Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 33.1)
  • TheSEED:  
    Two component system histidine kinase (EC 2.7.3.-) 
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 78.3)
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 46.3)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 20.3)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 19.6)
    no clan definedHAMP; HAMP domain (PF00672; HMM-score: 18.5)
    HIN; HIN-200/IF120x domain (PF02760; HMM-score: 17.8)
    DUF2969; Protein of unknown function (DUF2969) (PF11184; HMM-score: 15.2)
    Kinetocho_Slk19; Central kinetochore-associated (PF12709; HMM-score: 14.4)
    EI24; Etoposide-induced protein 2.4 (EI24) (PF07264; HMM-score: 14.2)
    DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 13.5)
    Peptidase_M48_N; CAAX prenyl protease N-terminal, five membrane helices (PF16491; HMM-score: 13.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.198
    • Ymax_pos: 13
    • Cmax: 0.228
    • Cmax_pos: 28
    • Smax: 0.525
    • Smax_pos: 7
    • Smean: 0.393
    • D: 0.274
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLTTIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSFNKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIKESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHENRTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIAISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDIDVGTTMTVTLHKLDITS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR
    SaeR (TF) important in Virulence  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Kathrin Rogasch, Vanessa Rühmling, Jan Pané-Farré, Dirk Höper, Christin Weinberg, Stephan Fuchs, Mareike Schmudde, Barbara M Bröker, Christiane Wolz, Michael Hecker, Susanne Engelmann
Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains.
J. Bacteriol.: 2006, 188(22);7742-58
[PubMed:17079681] [WorldCat.org] [DOI] (P p)
Julia Schmitt, Insa Joost, Eric P Skaar, Mathias Herrmann, Markus Bischoff
Haemin represses the haemolytic activity of Staphylococcus aureus in an Sae-dependent manner.
Microbiology (Reading, Engl.): 2012, 158(Pt 10);2619-31
[PubMed:22859613] [WorldCat.org] [DOI] (I p)