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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2569 [new locus tag: SACOL_RS13460 ]
  • pan locus tag?: SAUPAN006217000
  • symbol: SACOL2569
  • pan gene symbol?: rocA
  • synonym:
  • product: 1-pyrroline-5-carboxylate dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2569 [new locus tag: SACOL_RS13460 ]
  • symbol: SACOL2569
  • product: 1-pyrroline-5-carboxylate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2630234..2631778
  • length: 1545
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGTAGTAGAATTTAAAAATGAACCTGGTTACGATTTTTCAGTCCAAGAAAATGTGGAT
    ATGTTTAAGAAAGCATTGAAAGACGTGGAAAAAGAATTAGGTCAAGACATACCATTAGTG
    ATTAATGGAGAGAAGATTTTCAAAGATGACAAAATTAAATCAATCAATCCTGCAGACACA
    TCACAAGTGATTGCAAATGCATCAAAGGCAACAAAACAAGATGTTGAAGATGCGTTTAAA
    GCAGCAAACGAAGCTTATAAATCTTGGAAAACATGGTCAGCAAATGATCGTGCAGAGCTT
    ATGTTACGTGTTTCGGCAATTATTCGTCGTCGAAAAGCGGAAATTGCAGCAATTATGGTT
    TATGAAGCAGGTAAGCCATGGGATGAAGCAGTTGGTGATGCAGCTGAAGGTATTGATTTT
    ATTGAATACTATGCACGTTCAATGATGGACTTGGCTCAAGGTAAGCCAGTACTTGATCGT
    GAAGGTGAACACAATAAATATTTCTATAAATCAATTGGTACAGGTGTTACAATTCCGCCG
    TGGAACTTCCCGTTTGCGATTATGGCTGGTACAACTTTAGCACCAGTTGTGGCAGGTAAT
    ACAGTGTTATTGAAACCTGCTGAGGATACACCTTATATCGCTTATAAATTAATGGAAATT
    TTAGAAGAAGCAGGATTACCAAAAGGCGTAGTTAACTTTGTTCCTGGTGATCCGAAAGAA
    ATTGGTGATTACTTAGTTGATCATAAAGATACGCATTTCGTTACATTTACAGGATCACGT
    GCGACAGGTACTAGAATTTATGAACGTAGTGCCGTTGTTCAAGAAGGTCAAAACTTCTTG
    AAACGTGTTATCGCTGAAATGGGTGGTAAAGATGCCATTGTAGTTGATGAAAATATTGAT
    ACAGATATGGCAGCAGAAGCTATTGTAACGTCAGCATTTGGTTTTTCTGGTCAAAAATGT
    TCAGCATGTTCACGTGCAATTGTGCATAAAGACGTTTATGACGAAGTATTAGAGAAATCA
    ATCAAATTAACAAAAGAATTAACGTTGGGCAATACTGTAGACAATACGTATATGGGCCCA
    GTTATTAATAAAAAACAATTTGATAAGATTAAAAATTATATTGAGATTGGTAAAGAAGAA
    GGTAAATTAGAACAAGGTGGCGGCACTGATGATTCAAAAGGTTACTTTGTAGAACCAACA
    ATCATTTCAGGACTTAAATCTAAAGATAGAATCATGCAAGAAGAAATTTTTGGTCCAGTT
    GTTGGCTTTGTGAAAGTTAACGATTTTGATGAAGCAATTGAGGTTGCAAATGATACAGAT
    TACGGTTTAACTGGTGCAGTGATTACAAATAATCGCGAGCATTGGATTAAAGCGGTAAAC
    GAATTTGATGTAGGCAATTTATACCTTAACAGAGGCTGTACTTCAGCAGTTGTTGGATAC
    CATCCATTTGGTGGTTTCAAAATGTCAGGTACGGACGCTAAAACAGGTAGTCCAGACTAC
    TTATTACACTTCTTAGAACAAAAGGTTGTTTCAGAAATGTTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1545

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2569 [new locus tag: SACOL_RS13460 ]
  • symbol: SACOL2569
  • description: 1-pyrroline-5-carboxylate dehydrogenase
  • length: 514
  • theoretical pI: 4.723
  • theoretical MW: 56867.1
  • GRAVY: -0.290467

Function[edit source | edit]

  • reaction:
    EC 1.5.1.12?  ExPASy
    Transferred entry: 1.2.1.88
    EC 1.2.1.88?  ExPASy
    L-glutamate gamma-semialdehyde dehydrogenaseL-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesputative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 747.3)
    MetabolismEnergy metabolismAmino acids and amines1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 403)
    Cellular processesCellular processesAdaptations to atypical conditionsbetaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 338)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 327.6)
    MetabolismEnergy metabolismAmino acids and aminesdelta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 308.5)
    MetabolismCentral intermediary metabolismOthersuccinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 306.8)
    Unknown functionEnzymes of unknown specificityaldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 262.2)
    MetabolismEnergy metabolismOther2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 258.1)
    MetabolismEnergy metabolismAmino acids and aminesmethylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 254.5)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 241.4)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 241.2)
    MetabolismEnergy metabolismAmino acids and aminessuccinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 239.5)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 121.6)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 51.6)
    MetabolismAmino acid biosynthesisGlutamate familyglutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 20.5)
  • TheSEED:  
    Arginine and Ornithine Degradation Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88) 
    CBSS-196620.1.peg.2477 Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88) 
    Proline, 4-hydroxyproline uptake and utilization Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88) 
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 520.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL1199(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [1] (data from MRSA252)
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [1] (data from MRSA252)
    SACOL1103(pdhB)pyruvate dehydrogenase complex E1 component subunit beta  [1] (data from MRSA252)
    SACOL1104(pdhC)branched-chain alpha-keto acid dehydrogenase E2  [1] (data from MRSA252)
    SACOL1105(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SACOL1011(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SACOL1745(pyk)pyruvate kinase  [1] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SACOL2239(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SACOL2220(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SACOL1702(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SACOL2234(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SACOL1274(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SACOL1449(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    SACOL1448(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [1] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [1] (data from MRSA252)
    SACOL0944NADH dehydrogenase  [1] (data from MRSA252)
    SACOL1759universal stress protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 33
    • Cmax: 0.11
    • Cmax_pos: 49
    • Smax: 0.129
    • Smax_pos: 25
    • Smean: 0.086
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGEKIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELMLRVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDREGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDSKGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITNNREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQKVVSEMF

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [2] [3] [4] [5]
    quantitative data / protein copy number per cell: 45 [6]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  4. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]