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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01085 [old locus tag: SACOL0212 ]
  • pan locus tag?: SAUPAN001093000
  • symbol: SACOL_RS01085
  • pan gene symbol?: fadB
  • synonym:
  • product: 3-hydroxyacyl-CoA dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS01085 [old locus tag: SACOL0212 ]
  • symbol: SACOL_RS01085
  • product: 3-hydroxyacyl-CoA dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 249372..251633
  • length: 2262
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (249372..251633) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGACAATTAATAAAGTAACCGTTCTTGGCGCAGGCACAATGGGCGCTCAACTGGCAGCA
    CTTTTTGTGAATGCTGGACTTAAAGTAAAACTATTAGATATTGTAGTGGACAAAAACGAT
    CCAAATCTCATTGCGAAAAAATCTTACGATAAAATTACAGATAAGAAACGGCCGCTACTA
    TTCGACTTAAATCTAGCGAGTCATTTAACATATGGTAATTTTGATGATGACTTGGTAAAT
    GATGATGCTGATTTATATATCGAAGCAGTCAAAGAAGATATTGAAATTAAGCATGCTGTT
    TGGCAACAAGTTCTACAACATGCTAAAGAAGATGCTTTATTCGCTACAAATACATCAGGT
    ATTCCAATTAATGCGATTGCTCAAGCATTTAACGAGAAGGATCAAGAACGATTCTTTGGT
    CTACATTTCTTTAACCCACCACGTATTATGAAATTAGTGGAGTTAATACCTACGTCACAC
    ACGAAGGAATCTATTATATTAGATGTAAAAAATTTCGCGCAAAATGTGTTAGGTAAAGGT
    GTCATTGTCGTCAATGATGTGCCTGGCTTTGTCGCAAATAGAGTCGGCACGCAAACAATG
    AATGATATTATGTATCGCGCCGAGCAACACAAGATAAGCATTGTAGATGTGGATGCTTTA
    ACTGGGCAAGCGATTGGTCGTCCTAAAACAGGTACATATGCGCTATCTGACCTAGTCGGT
    TTAGATATTGCAGTGTCTGTAATTAAAGGCATGCAACAAGTACCTGAAGAAACACCTTAT
    TTTCATGATGTCAAAATTGTAAATACGTTGTTTGACAATGGCGCACTCGGACGTAAAACG
    AAACAAGGATTTTACAAAAAGGATAAAGAAACTAAAGCTCGACTTGTTTACGATGTTGAA
    AAACAAGATTATGTACCTGTATCGCAACCACAATTACCAATTTTAAATGAATTTAATAAA
    GACTTAGTGCATAACCTTGATACCATATTCAATGCGCAAGACGAAGCGGGACTATTTTTA
    TGGGAGACATTACGTAATAATTTCTATTACTCTGCTATCAATGTACCTAAAGCTACCGAT
    GATTTCCGAGACATAGACCGTGCGCTTGTCTGGGGGTTCAACTGGAAACTTGGTCCATTC
    CAATTATGGGATGCAATGGGATACGAACGTGTTAAAACACGTATGGAAGACGAACTTGGA
    GACTTACCACAATGGATTAGTGATTTAGATGGTGGCTTTTATAAACAAGATGAGACCATT
    GAATATGCAACACCTATTTCTCACTTCGTAAAAGATGAACTTTGGGATAAAGGTGATGCC
    AAACTTTCCGTAACTCATGATGATCAACTGTTACTGAAATTACAAAGTAAAAATAATGTC
    ATTACCGATGAATTCAACGATGCGTTAGTTGATGCGATTGATTTACTGGAAAATGACCAT
    TACACAAGTATGGTTATTTATGCAGATGGTAACAATTTCAGTGTGGGTGCTAACCTTTTC
    TTAATGAAAAAGGCGCATGAAGACGGTCTTGTAGATGATGTCGTTGCACAATCAATTGAT
    AAATTACATTATAGCTTTAATCGTTTGAAGTATAGTTTGAAACCAGTAGTCACAGCTGTT
    CAAGGTCGTGCCTTAGGCGGTGGCTGTGAGCTTGTACTTTACTCACCTATTGTTGTCGCT
    GCAAGTGAAACATATATCGGTCTTGTTGAAGCAGGTGTTGGCTTATTACCGAGTGGCGGT
    GGCCTTGCAGAAATGGCTGATCGCATATTACGCACATCGCATAAGTTTGATGACAAACAA
    GCTTCCATGACAAAAGTACTGACGAATATCGCATTTGCGAAAGTCTCTACAAATGCCTTT
    GAGGCACGTCGTTATGGTTATTTACGTGATACAGATACGATTATTTTCAATACAGCACAA
    CGTGTCGAAGTTGCGCTCAAACGTGCGAAATATGAAGCAGAAACAAACTATATTCCGAAT
    CCTAGACATCAATATATCGCTTTAGGTGAAGACTTCAAAGCATTGATCCAAGGACAATTA
    GATGCGCAAAGACGGGGTCATTTTATTAGCGACCATGATTATCATATTGCCTTAAATATC
    GCCACAATTTTAGCGGGTGGTGATTTACCAAGAAATACATTTATCAATCAACGTTACATT
    CAATCGTTGGAGAAAATTGGCTTTATTGACTTACTAAAATCTAAAAAATCATATGAAAGA
    ATTGCACATATGTTAAAAACTGGTAAGCCATTACGTAATTAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS01085 [old locus tag: SACOL0212 ]
  • symbol: SACOL_RS01085
  • description: 3-hydroxyacyl-CoA dehydrogenase
  • length: 753
  • theoretical pI: 5.83805
  • theoretical MW: 84607.8
  • GRAVY: -0.242762

Function[edit source | edit]

  • reaction:
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH?
  • TIGRFAM:
    fatty acid oxidation complex, alpha subunit, mitochondrial (TIGR02441; EC 1.1.1.35,4.2.1.17; HMM-score: 174.9)
    MetabolismFatty acid and phospholipid metabolismDegradationfatty oxidation complex, alpha subunit FadB (TIGR02437; EC 1.1.1.35,4.2.1.17,5.1.2.3,5.3.3.8; HMM-score: 168.1)
    MetabolismFatty acid and phospholipid metabolismDegradationfatty oxidation complex, alpha subunit FadJ (TIGR02440; EC 1.1.1.35,4.2.1.17,5.1.2.3; HMM-score: 154.8)
    3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 135.1)
    phenylacetate degradation probable enoyl-CoA hydratase PaaB (TIGR02280; EC 4.2.1.17; HMM-score: 41.4)
    cyclohexa-1,5-dienecarbonyl-CoA hydratase (TIGR03189; EC 4.2.1.100; HMM-score: 29)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonenaphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 18)
    2-ketocyclohexanecarboxyl-CoA hydrolase (TIGR03210; EC 3.7.-.-; HMM-score: 17.4)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 14.1)
    MetabolismAmino acid biosynthesisGlutamate familypyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 13.7)
    MetabolismEnergy metabolismAmino acids and aminesalanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 13.4)
    benzoyl-CoA-dihydrodiol lyase (TIGR03222; EC 4.1.2.44; HMM-score: 12.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 12.1)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) 3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 175.1)
    6PGD_C (CL0106) 3HCDH; 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain (PF00725; HMM-score: 94.2)
    ClpP_crotonase (CL0127) ECH_1; Enoyl-CoA hydratase/isomerase (PF00378; HMM-score: 60.3)
    ECH_2; Enoyl-CoA hydratase/isomerase (PF16113; HMM-score: 33.4)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 14.8)
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 13.5)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 13)
    no clan definedYscW; Type III secretion system lipoprotein chaperone (YscW) (PF09619; HMM-score: 12.8)
    NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 12.5)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL_RS04845cytosol aminopeptidase  [1] (data from MRSA252)
    SACOL_RS05630pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SACOL_RS0868050S ribosomal protein L21  [1] (data from MRSA252)
    SACOL_RS09125formate--tetrahydrofolate ligase  [1] (data from MRSA252)
    SACOL_RS1175550S ribosomal protein L22  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.206
    • Ymax_pos: 20
    • Cmax: 0.132
    • Cmax_pos: 26
    • Smax: 0.452
    • Smax_pos: 10
    • Smean: 0.358
    • D: 0.265
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446076659 NCBI
  • RefSeq: WP_000154514 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLIAKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQQVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSHTKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVDALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGALGRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQDEAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVKTRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQLLLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHEDGLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYIGLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARRYGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDAQRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYERIAHMLKTGKPLRN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS01085 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4

Relevant publications[edit source | edit]