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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05630 [old locus tag: SACOL1102 ]
  • pan locus tag?: SAUPAN003317000
  • symbol: SACOL_RS05630
  • pan gene symbol?: pdhA
  • synonym:
  • product: pyruvate dehydrogenase E1 component subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS05630 [old locus tag: SACOL1102 ]
  • symbol: SACOL_RS05630
  • product: pyruvate dehydrogenase E1 component subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1112070..1113182
  • length: 1113
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1112070..1113182) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGCTCCTAAGTTACAAGCCCAATTCGATGCAGTAAAAGTTTTAAATGATACTCAATCG
    AAATTTGAAATGGTTCAAATTTTGGATGAGAATGGTAACGTCGTAAATGAAGACTTAGTA
    CCTGATCTTACGGATGAACAATTAGTGGAATTAATGGAAAGAATGGTATGGACTCGTATC
    CTTGATCAACGTTCTATCTCATTAAACAGACAAGGACGTTTAGGTTTCTATGCACCAACT
    GCTGGTCAAGAAGCATCACAATTAGCGTCACAATACGCTTTAGAAAAAGAAGATTACATT
    TTACCGGGATACAGAGATGTTCCTCAAATTATTTGGCATGGTTTACCATTAACTGAAGCT
    TTCTTATTCTCAAGAGGTCACTTCAAAGGAAATCAATTCCCTGAAGGCGTTAATGCATTA
    AGCCCACAAATTATTATCGGTGCACAATACATTCAAGCTGCTGGTGTTGCATTTGCACTT
    AAAAAACGTGGTAAAAATGCAGTTGCAATCACTTACACTGGTGACGGTGGTTCTTCACAA
    GGTGATTTCTACGAAGGTATTAACTTTGCAGCAGCTTATAAAGCACCTGCAATTTTCGTT
    ATTCAAAACAATAACTATGCAATTTCAACACCAAGAAGCAAGCAAACTGCTGCTGAAACA
    TTAGCTCAAAAAGCAATTGCTGTAGGTATTCCTGGTATCCAAGTTGATGGTATGGATGCG
    TTAGCTGTATATCAAGCAACTAAAGAAGCACGTGACCGCGCAGTTGCAGGTGAAGGTCCA
    ACATTAATTGAAACTATGACATATCGTTATGGTCCTCATACAATGGCTGGTGACGATCCA
    ACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGT
    TTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATT
    GAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAA
    ACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGCCTCAAAACTTAGCAGAACAA
    TATGAAATTTACAAAGAGAAGGAGTCGAAGTAA
    60
    120
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    420
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    720
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    840
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    1020
    1080
    1113

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS05630 [old locus tag: SACOL1102 ]
  • symbol: SACOL_RS05630
  • description: pyruvate dehydrogenase E1 component subunit alpha
  • length: 370
  • theoretical pI: 4.62739
  • theoretical MW: 41382.3
  • GRAVY: -0.484054

Function[edit source | edit]

  • reaction:
    EC 1.2.4.1?  ExPASy
    Pyruvate dehydrogenase (acetyl-transferring)Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 488)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 217.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 21.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 21.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 21.6)
    MetabolismEnergy metabolismTCA cycleoxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 16.7)
  • TheSEED:
  • PFAM:
    THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 345.3)
    DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 21.7)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 18.7)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 31
    • Cmax: 0.113
    • Cmax_pos: 21
    • Smax: 0.16
    • Smax_pos: 22
    • Smean: 0.109
    • D: 0.112
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445957465 NCBI
  • RefSeq: WP_000035320 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLTDEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILPGYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFALKKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAAETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMAGDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEADNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS05630 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]