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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13750 [old locus tag: SACOL2627 ]
  • pan locus tag?: SAUPAN006313000
  • symbol: SACOL_RS13750
  • pan gene symbol?: betA
  • synonym:
  • product: oxygen-dependent choline dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13750 [old locus tag: SACOL2627 ]
  • symbol: SACOL_RS13750
  • product: oxygen-dependent choline dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2685993..2687702
  • length: 1710
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2685993..2687702) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    ATGAGTAACAAAAACAAATCATATGATTATGTCATCATTGGAGGAGGCAGTGCAGGTTCT
    GTACTAGGTAATCGTCTGAGTGAAGATAAAGATAAAGAAGTCTTAGTATTAGAAGCGGGT
    CGCAGTGATTATTTTTGGGATTTATTTATCCAAATGCCTGCTGCGTTAATGTTCCCTTCA
    GGCAATAAATTTTACGATTGGATTTATTCAACAGATGAAGAACCACATATGGGCGGTCGT
    AAAGTAGCACATGCCCGAGGTAAAGTTTTAGGTGGATCAAGTTCGATTAATGGCATGATT
    TATCAACGTGGTAATCCAATGGACTATGAAGGCTGGGCAGAACCAGAAGGTATGGAAACT
    TGGGATTTTGCGCACTGTTTACCGTATTTTAAAAAATTAGAAAAAACATACGGTGCAGCG
    CCTTATGATAAATTTAGAGGCCATGATGGACCAATTAAGTTAAAACGAGGGCCAGCAACG
    AATCCTTTATTCCAGTCATTCTTTGATGCAGGTGTTGAAGCAGGCTATCATAAAACACCT
    GATGTGAATGGATTTAGACAAGAAGGTTTTGGACCGTTCGATAGTCAAGTACATCGTGGT
    CGCCGAATGTCAGCTTCAAGAGCATATTTACATCCAGCGATGAAGCGTAAAAACTTAACC
    GTTGAAACACGTGCCTTTGTAACTGAAATTCATTATGAAGGTAGAAGAGCAACTGGTGTT
    ACGTATAAGAAAAATGGCAAACTACATACCATCGATGCTAATGAAGTCATTTTGTCTGGT
    GGGGCATTCAATACGCCACAATTACTACAATTATCTGGTATCGGTGATTCAGAGTTCCTA
    AAATCAAAAGGCATTGAGCCACGTGTTCATTTACCTGGTGTGGGTGAAAACTTTGAAGAT
    CACTTAGAGGTATACATTCAACATAAATGTAAGGAACCTGTATCATTACAGCCAAGCTTA
    GATATCAAGCGCATGCCATTTATTGGTTTACAATGGATTTTCACACGTACAGGTGCAGCA
    GCATCTAACCATTTTGAAGGTGGCGGATTTGTACGTTCAAATAACGAAGTTGATTATCCT
    AACTTAATGTTCCATTTCTTACCAATAGCTGTAAGATATGATGGTCAAAAGGCAGCGGTC
    GCACATGGTTATCAAGTTCACGTAGGACCAATGTATTCAAACTCTCGTGGTAGCTTGAAA
    ATCAAATCTAAAGATCCATTCGAAAAACCAAGTATCCGCTTTAATTATTTATCAACAGAA
    GAAGATAAAAAAGAATGGGTAGAAGCAATTCGTGTAGCAAGAAATATCTTATCTCAAAAA
    GCAATGGATCCATTCAATGGTGGAGAAATTTCACCAGGACCAGAAGTGCAAACAGACGAA
    GAAATTCTTGATTGGGTTCGTAGAGATGGTGAAACAGCATTACACCCATCTTGTAGTGCT
    AAAATGGGTCCTGCTTCAGATCCAATGGCAGTAGTTGACCCGTTAACAATGAAAGTCCAC
    GGTATGGAAAATTTACGTGTAGTTGATGCATCAGCTATGCCACGTACGACAAATGGCAAT
    ATACACGCACCTGTTTTAATGTTAGCTGAAAAAGCAGCAGACATTATACGTGGCAGAAAG
    CCTTTAGAGCCACAATATATTGATTATTATAAGCATGGTGTTCATGATGAAAATGAAGGT
    GCAATCGAAGTAAAACCATACGCTAAATAA
    60
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    1710

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13750 [old locus tag: SACOL2627 ]
  • symbol: SACOL_RS13750
  • description: oxygen-dependent choline dehydrogenase
  • length: 569
  • theoretical pI: 7.40617
  • theoretical MW: 63609.6
  • GRAVY: -0.541476

Function[edit source | edit]

  • reaction:
    EC 1.2.1.8?  ExPASy
    Betaine-aldehyde dehydrogenaseBetaine aldehyde + NAD+ + H2O = betaine + NADH
    EC 1.1.99.1?  ExPASy
    Choline dehydrogenaseCholine + acceptor = betaine aldehyde + reduced acceptor
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionscholine dehydrogenase (TIGR01810; EC 1.1.99.1; HMM-score: 934.7)
    dehydrogenase, Rv0697 family (TIGR03970; EC 1.-.-.-; HMM-score: 321.7)
    Unknown functionEnzymes of unknown specificityGMC family mycofactocin-associated oxidreductase (TIGR04542; EC 1.-.-.-; HMM-score: 115.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesL-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 17.4)
    Unknown functionEnzymes of unknown specificityputative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 15.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 14.9)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesUDP-galactopyranose mutase (TIGR00031; EC 5.4.99.9; HMM-score: 14.8)
    Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 12.2)
    MetabolismEnergy metabolismElectron transportflavocytochrome c (TIGR01813; HMM-score: 12.1)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 11.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 11.1)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) GMC_oxred_N; GMC oxidoreductase (PF00732; HMM-score: 263.4)
    no clan definedGMC_oxred_C; GMC oxidoreductase (PF05199; HMM-score: 125.9)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 30.1)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 25)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 19.1)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 17.7)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 17.3)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 14.1)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.139
    • Ymax_pos: 11
    • Cmax: 0.151
    • Cmax_pos: 28
    • Smax: 0.228
    • Smax_pos: 4
    • Smean: 0.197
    • D: 0.161
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445988666 NCBI
  • RefSeq: WP_000066521 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDYFWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQRGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPATNPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKNLTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGDSEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIFTRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMYSNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEISPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVVDASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKPYAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13750 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]