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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01142
  • pan locus tag?: SAUPAN003448000
  • symbol: SAOUHSC_01142
  • pan gene symbol?: mraZ
  • synonym:
  • product: cell division protein MraZ

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01142
  • symbol: SAOUHSC_01142
  • product: cell division protein MraZ
  • replicon: chromosome
  • strand: +
  • coordinates: 1092096..1092527
  • length: 432
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGTTCATGGGAGAATACGATCATCAATTAGATACAAAAGGACGTATGATTATACCGTCC
    AAGTTTCGTTATGACTTAAATGAGCGTTTTATTATCACAAGAGGCCTTGATAAATGTTTA
    TTCGGTTACACTCTAGACGAATGGCAACAGATTGAAGAGAAAATGAAAACCTTACCTATG
    ACAAAAAAAGACGCACGTAAGTTTATGCGTATGTTCTTCTCTGGTGCTGTTGAAGTAGAA
    CTTGATAAGCAAGGGCGTATTAACATCCCTCAAAACTTGAGGAAATACGCTAATTTAACT
    AAAGAATGTACAGTAATCGGTGTTTCAAATCGTATTGAGATTTGGGATAGAGAAACTTGG
    AATGATTTCTATGAAGAATCTGAAGAAAGTTTCGAAGATATTGCTGAAGATTTAATAGAT
    TTTGATTTTTAA
    60
    120
    180
    240
    300
    360
    420
    432

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01142
  • symbol: SAOUHSC_01142
  • description: cell division protein MraZ
  • length: 143
  • theoretical pI: 4.52156
  • theoretical MW: 17237.5
  • GRAVY: -0.646154

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesCell divisiondivision/cell wall cluster transcriptional repressor MraZ (TIGR00242; HMM-score: 185.8)
    Signal transductionRegulatory functionsDNA interactionsdivision/cell wall cluster transcriptional repressor MraZ (TIGR00242; HMM-score: 185.8)
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator, AbrB family (TIGR01439; HMM-score: 22.8)
    Hypothetical proteinsConservedTIGR02922 family protein (TIGR02922; HMM-score: 14.1)
  • TheSEED:  
    Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryBacterial Cytoskeleton Cell division protein MraZ 
    Bacterial Cell Division Cell division protein MraZ 
  • PFAM:
    AbrB (CL0132) MraZ; MraZ protein, putative antitoxin-like (PF02381; HMM-score: 175.3)
    no clan definedDUF2375; Protein of unknown function (DUF2375) (PF09558; HMM-score: 14.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.092
    • Ymax_pos: 70
    • Cmax: 0.104
    • Cmax_pos: 52
    • Smax: 0.101
    • Smax_pos: 4
    • Smean: 0.071
    • D: 0.084
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYTLDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKECTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]