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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01147
  • pan locus tag?: SAUPAN003453000
  • symbol: murD
  • pan gene symbol?: murD
  • synonym:
  • product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01147
  • symbol: murD
  • product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1097367..1098716
  • length: 1350
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCTTAATTATACAGGGTTAGAAAATAAAAATGTATTAGTTGTCGGTTTGGCAAAAAGT
    GGTTATGAAGCAGCTAAATTATTAAGTAAATTAGGTGCGAATGTAACTGTCAATGATGGA
    AAAGACTTATCACAAGATGCTCATGCAAAAGATTTAGAATCTATGGGCATTTCTGTTGTA
    AGTGGAAGTCATCCATTAACGTTGCTTGATAATAATCCAATAATTGTTAAAAATCCTGGA
    ATACCTTATACAGTATCTATTATTGATGAAGCAGTGAAACGAGGTTTGAAAATTTTAACA
    GAAGTTGAGTTAAGTTATCTAATCTCTGAAGCACCAATCATAGCTGTAACGGGTACAAAT
    GGTAAAACGACAGTTACTTCTCTAATTGGAGATATGTTTAAAAAAAGTCGCTTAACTGGA
    AGATTATCCGGCAATATTGGTTATGTTGCATCTAAAGTAGCACAAGAAGTAAAGCCTACA
    GATTATTTAGTTACAGAGTTGTCGTCATTCCAGTTACTTGGAATCGAAAAGTATAAACCA
    CACATTGCTATAATTACTAACATTTATTCGGCGCATCTAGATTACCATGAAAATTTAGAA
    AACTATCAAAATGCTAAAAAGCAAATATATAAAAATCAAACGGAAGAGGATTATTTGATT
    TGTAATTATCATCAAAGACAAGTGATAGAGTCGGAAGAATTAAAAGCTAAGACATTGTAT
    TTCTCAACTCAACAAGAAGTTGATGGTATTTATATTAAAGATGGTTTTATCGTTTATAAA
    GGTGTTCGTATTATTAACACTGAAGATCTAGTATTGCCTGGTGAACATAATTTAGAAAAT
    ATATTAGCAGCTGTGCTTGCTTGTATTTTAGCTGGTGTACCTATTAAAGCAATTATTGAT
    AGTTTAACTACATTTTCAGGAATAGAGCATAGATTGCAATATGTTGGTACTAATAGAACT
    AATAAATATTATAATGATTCCAAAGCAACAAACACGCTAGCAACACAGTTTGCCTTAAAT
    TCATTTAATCAACCAATCATTTGGTTATGTGGTGGTTTGGATCGAGGGAATGAATTTGAC
    GAACTCATTCCTTATATGGAAAATGTTCGCGCGATGGTTGTATTCGGACAAACGAAAGCT
    AAGTTTGCTAAACTAGGTAATAGTCAAGGGAAATCGGTCATTGAAGCGAACAATGTCGAA
    GACGCTGTTGATAAAGTACAAGATATTATAGAACCAAATGATGTTGTATTATTGTCACCT
    GCTTGTGCGAGTTGGGATCAATATAGTACTTTTGAAGAGCGTGGAGAGAAATTTATTGAA
    AGATTCCGTGCCCATTTACCATCTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1350

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01147
  • symbol: MurD
  • description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
  • length: 449
  • theoretical pI: 5.49171
  • theoretical MW: 49843.4
  • GRAVY: -0.167038

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.2.9?  ExPASy
    UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 415.2)
    and 10 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 131.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 86)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 83.6)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 49.6)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 34.3)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 24.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 17.7)
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 13.9)
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 13.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 12.8)
  • TheSEED  :
    • UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (EC 6.3.2.9)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan biosynthesis--gjo  UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)
  • PFAM:
    no clan defined Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 117.2)
    and 7 more
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 37.9)
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 15.7)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.4)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 15.2)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.2)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 14.6)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019906
    • TAT(Tat/SPI): 0.00131
    • LIPO(Sec/SPII): 0.001645
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLSQDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEVELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPTDYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKSVIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_00520(rplJ)50S ribosomal protein L10  [4] (data from MRSA252)
    SAOUHSC_00679hypothetical protein  [4] (data from MRSA252)
    SAOUHSC_01806pyruvate kinase  [4] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ 5.0 5.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Delphine Patin, Audrey Boniface, Andreja Kovač, Mireille Hervé, Sébastien Dementin, Hélène Barreteau, Dominique Mengin-Lecreulx, Didier Blanot
Purification and biochemical characterization of Mur ligases from Staphylococcus aureus.
Biochimie: 2010, 92(12);1793-800
[PubMed:20659527] [WorldCat.org] [DOI] (I p)

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