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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01169
  • pan locus tag?: SAUPAN003482000
  • symbol: SAOUHSC_01169
  • pan gene symbol?: carA
  • synonym:
  • product: carbamoyl phosphate synthase small subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01169
  • symbol: SAOUHSC_01169
  • product: carbamoyl phosphate synthase small subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 1117857..1118957
  • length: 1101
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCAAAGCAAACGTTATCTAGTGTTAGAAGACGGTTCTTTTTACGAAGGCTACCGTTTA
    GGATCTGATAACTTAACTGTAGGAGAAATTGTATTTAATACAGCGATGACAGGTTATCAA
    GAAACTATTTCAGATCCATCATATACAGGTCAGATCATTACTTTTACGTATCCATTAATC
    GGTAATTATGGTATCAATAGAGACGATTTTGAATCATTAGTACCTACATTAAACGGTATT
    GTAGTGAAAGAAGCGAGTGCGCATCCAAGTAATTTTAGACAGCAAAAGACACTTCATGAC
    GTTTTAGAATTGCATCAAATTCCAGGGATTGCAGGTGTTGATACAAGAAGTATTACGCGT
    AAAATTCGACAACACGGTGTGTTAAAAGCTGGTTTTACTGATCGAAAAGAAGATATTGAT
    CAACTTGTCAAACATTTACAACAAGTAGAATTACCTAAAAACGAAGTAGAAATCGTTTCG
    ACTAAAACACCGTATGTTTCGACAGGTAAGGATCTAAGTGTCGTACTTGTAGACTTTGGT
    AAGAAGCAAAATATTGTTCGAGAATTAAACGTCAGAGGTTGTAACGTCACAGTTGTACCA
    TATACAACTACTGCCGAAGAAATTTTAGCAATGGCTCCAGATGGCGTTATGCTATCAAAC
    GGACCAGGTAATCCTGAAGTTGTAGAATGTGCGATTCCAATGATTCAAGGAATTTTAGGG
    AAAATTCCGTTCTTTGGTATCTGTCTAGGACATCAACTTTTTGCATTATCTCAAGGAGCA
    AGCTCATTTAAAATGAAGTTTGGTCATCGTGGTGCGAACCATCCAGTTAAAAATTTAGAG
    ACTGGAAAAGTTGATATTACGAGTCAAAACCATGGATATGCAATAGATATAGATTCGTTA
    AAAAGTACTGATTTAGAAGTTACTCATCTTGCATTAAATGATGGTACTGTAGAAGGTTTA
    AAACATAAAACATTACCAGCATTTTCTGTTCAATACCATCCTGAAGCAAATCCAGGACCG
    TCAGATTCAAACTATCTATTTGATGATTTTGTAGCAATGATGACTAATTTTAAGGAAAAG
    GAGCGTCATATCAATGCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1101

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01169
  • symbol: SAOUHSC_01169
  • description: carbamoyl phosphate synthase small subunit
  • length: 366
  • theoretical pI: 6.18896
  • theoretical MW: 40393.6
  • GRAVY: -0.25847

Function[edit source | edit]

  • reaction:
    EC 6.3.5.5?  ExPASy
    Carbamoyl-phosphate synthase (glutamine-hydrolyzing)2 ATP + L-glutamine + HCO3(-) + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesiscarbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 484.5)
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 62.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisGMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 59.9)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 50.1)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 48.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisCTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 20)
  • TheSEED:  
    Nucleosides and NucleotidesPyrimidinesDe Novo Pyrimidine Synthesis Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) 
  • PFAM:
    Leu-IlvD (CL0364) CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain (PF00988; HMM-score: 167.7)
    Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 162.1)
    Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 30.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.111
    • Ymax_pos: 41
    • Cmax: 0.115
    • Cmax_pos: 41
    • Smax: 0.141
    • Smax_pos: 33
    • Smean: 0.092
    • D: 0.104
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVSTKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]