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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01724
  • pan locus tag?: SAUPAN004218000
  • symbol: SAOUHSC_01724
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01724
  • symbol: SAOUHSC_01724
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1630711..1631379
  • length: 669
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATAGATCAACAAACAATTTATCAATACATACAAAATGGAAAAATAGAAGAAGCGTTA
    CAAGCATTGTTCGGAAATATCGAAGAAAATCCTACAATTATTGAAAATTATATTAATGCT
    GGTATCGTACTTGCTGATGCGAATGAGATTGAAAAGGCAGAGCGTTTTTTCCAAAAAGCT
    TTAACAATAGATCCGAAGAATGGCGTCGTATTTTTTAATCTAGCAAATGTATATTATAAT
    CAGCAACGTTATCAAGAAGCTATTAAATTATATCAACAAGCATTACAAACAGAGATTGAA
    CAAGTTGATTGTAATTATATGATCGGTATGGCGTTTAATCAGTTAGAATCATTTAAGCTG
    GCATTGCCGTATTTAATGACTGCTGCGGAACTAGATAAAGACAAAGATGCAGAAGTTCAA
    TTTCAATATGGTCTTGTATTATGTCAATTAGAAATGTTTAATGAAGCCATAACTCAACTT
    AAACATGTATTAACGATTGATAAAAATCATGTTGATGCAAGATACAATTTGGGCTTAGCG
    TTATTTATGAAAAATGAAGATATTGATGAAGCAATAACTCATTTTAAAGAAGCTGTGACT
    ATCGACCCTAAACACTTATTAAGTCAGCATGCGCTGAAAACATTCACTAAAATGAAAGAG
    GAGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    669

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01724
  • symbol: SAOUHSC_01724
  • description: hypothetical protein
  • length: 222
  • theoretical pI: 4.41965
  • theoretical MW: 25701.1
  • GRAVY: -0.277477

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 72.1)
    putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 70.8)
    type III secretion low calcium response chaperone LcrH/SycD (TIGR02552; HMM-score: 56.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesmitochondrial precursor proteins import receptor (TIGR00990; HMM-score: 49.8)
    tol-pal system protein YbgF (TIGR02795; HMM-score: 31.4)
    putative peptide modification system cyclase (TIGR04510; HMM-score: 26.2)
    Unknown functionGeneralheme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 24.5)
    pentatricopeptide repeat domain (TIGR00756; HMM-score: 21.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesadenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 21.4)
    poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (TIGR03939; HMM-score: 20.7)
  • TheSEED:  
    Tetratricopeptide repeat (TPR) family protein 
  • PFAM:
    TPR (CL0020) TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 100)
    TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 99.7)
    TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 93.7)
    TPR_11; TPR repeat (PF13414; HMM-score: 86.9)
    TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 80.2)
    TPR_17; Tetratricopeptide repeat (PF13431; HMM-score: 64.5)
    TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 63.2)
    TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 59.9)
    TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 59.4)
    ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 53.3)
    TPR_7; Tetratricopeptide repeat (PF13176; HMM-score: 52.4)
    TPR_10; Tetratricopeptide repeat (PF13374; HMM-score: 47.6)
    TPR_6; Tetratricopeptide repeat (PF13174; HMM-score: 42.5)
    TPR_20; Tetratricopeptide repeat (PF14561; HMM-score: 35.5)
    TOM20_plant; Plant specific mitochondrial import receptor subunit TOM20 (PF06552; HMM-score: 28.3)
    TPR_9; Tetratricopeptide repeat (PF13371; HMM-score: 28.1)
    TPR_3; Tetratricopeptide repeat (PF07720; HMM-score: 26.8)
    PPR; PPR repeat (PF01535; HMM-score: 24.8)
    TPR_15; Tetratricopeptide repeat (PF13429; HMM-score: 22.2)
    no clan definedMIT; MIT (microtubule interacting and transport) domain (PF04212; HMM-score: 21.5)
    TPR (CL0020) PPR_2; PPR repeat family (PF13041; HMM-score: 18)
    NARP1; NMDA receptor-regulated protein 1 (PF12569; HMM-score: 16.8)
    TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 16.4)
    TPR_4; Tetratricopeptide repeat (PF07721; HMM-score: 15.8)
    Hect (CL0552) HECT_2; HECT-like Ubiquitin-conjugating enzyme (E2)-binding (PF09814; HMM-score: 15.3)
    TPR (CL0020) Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 13)
    Clathrin; Region in Clathrin and VPS (PF00637; HMM-score: 12.1)
    Sel1; Sel1 repeat (PF08238; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 28
    • Cmax: 0.115
    • Cmax_pos: 46
    • Smax: 0.127
    • Smax_pos: 18
    • Smean: 0.085
    • D: 0.094
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVLADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQVDCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQLKHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKMKEEE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]