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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS03510 [old locus tag: NWMN_0617 ]
- pan locus tag?: SAUPAN002526000
- symbol: NWMN_RS03510
- pan gene symbol?: fhuB
- synonym:
- product: iron ABC transporter permease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS03510 [old locus tag: NWMN_0617 ]
- symbol: NWMN_RS03510
- product: iron ABC transporter permease
- replicon: chromosome
- strand: +
- coordinates: 699322..700326
- length: 1005
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGACAAATAGAGAGAACCCAACGCCATTGAAGTTTTTATCCTATATTATAGGTTTAAGT
ATGATACTACTAATCACACTATTTATTTCTACATTAATAGGTGACGCCAAAATTCAAGCC
TCTACAATTATAGAGGCTATTTTTAATTATAATCCTAGCAATCAACAGCAAAACATCATC
AATGAGATTAGGATTCCCAGAAATATAGCAGCAGTAATTGTAGGTATGGCGCTTGCAGTT
TCTGGTGCGATTATACAAGGTGTTACTCGTAATGGTCTTGCTGATCCGGCGCTCATAGGT
TTAAATTCAGGTGCTTCATTTGCTTTAGCATTAACATATGCAGTTTTACCAAACACTTCA
TTTTTAATATTGATGTTTGCTGGATTTTTAGGTGCTATTCTAGGAGGTGCTATTGTATTA
ATGATAGGCCGATCTAGACGTGATGGATTTAATCCGATGCGTATTATTTTAGCGGGTGCA
GCAGTAAGTGCTATGTTAACAGCGCTAAGTCAAGGTATTGCATTAGCTTTTAGACTAAAT
CAAACAGTAACATTTTGGACTGCTGGAGGCGTTTCAGGCACAACATGGTCACACCTTAAG
TGGGCAATTCCATTAATTGGTATTGCGTTATTCATTATATTAACAATTAGTAAACAACTT
ACCATTTTAAATCTTGGTGAATCATTAGCTAAAGGTTTAGGTCAAAATGTAACAATGATC
AGAGGCATATGTTTAATTATTGCTATGATTCTAGCAGGTATTGCAGTTGCTATCGCTGGA
CAAGTTGCATTTGTAGGTTTGATGGTACCTCATATAGCAAGATTTTTAATTGGAACTGAT
TATGCTAAAATTCTACCATTAACAGCCTTGTTAGGTGGGATACTCGTGCTTGTTGCCGAT
GTGATAGCACGATATTTAGGAGAAGCGCCTGTTGGTGCAATCATTTCATTTATCGGTGTT
CCTTACTTTTTATATTTAGTTAAAAAAGGAGGACGCTCAATATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS03510 [old locus tag: NWMN_0617 ]
- symbol: NWMN_RS03510
- description: iron ABC transporter permease
- length: 334
- theoretical pI: 10.6575
- theoretical MW: 35183
- GRAVY: 0.973952
⊟Function[edit | edit source]
- TIGRFAM: proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 156.1)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 295)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 9
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.402386
- TAT(Tat/SPI): 0.006407
- LIPO(Sec/SPII): 0.011721
- predicted transmembrane helices (TMHMM): 9
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTIIEAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLNSGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGAAVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISKQLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFLIGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
NWMN_RS11780 (deoA) pyrimidine-nucleoside phosphorylase [1] (data from MRSA252) NWMN_RS10565 (gatA) glutamyl-tRNA(Gln) amidotransferase subunit A [1] (data from MRSA252) NWMN_RS00045 serine--tRNA ligase [1] (data from MRSA252) NWMN_RS00885 formate acetyltransferase [1] (data from MRSA252) NWMN_RS00980 L-lactate dehydrogenase [1] (data from MRSA252) NWMN_RS02020 30S ribosomal protein S6 [1] (data from MRSA252) NWMN_RS02030 30S ribosomal protein S18 [1] (data from MRSA252) NWMN_RS02155 GMP synthase (glutamine-hydrolyzing) [1] (data from MRSA252) NWMN_RS02630 stage V sporulation protein G [1] (data from MRSA252) NWMN_RS02655 50S ribosomal protein L25/general stress protein Ctc [1] (data from MRSA252) NWMN_RS02690 RNA-binding protein S1 [1] (data from MRSA252) NWMN_RS02815 pyridoxal 5'-phosphate synthase lyase subunit PdxS [1] (data from MRSA252) NWMN_RS02910 50S ribosomal protein L11 [1] (data from MRSA252) NWMN_RS02915 50S ribosomal protein L1 [1] (data from MRSA252) NWMN_RS02920 50S ribosomal protein L10 [1] (data from MRSA252) NWMN_RS02925 50S ribosomal protein L7/L12 [1] (data from MRSA252) NWMN_RS02935 DNA-directed RNA polymerase subunit beta [1] (data from MRSA252) NWMN_RS02950 30S ribosomal protein S12 [1] (data from MRSA252) NWMN_RS02960 elongation factor G [1] (data from MRSA252) NWMN_RS03300 zinc-dependent alcohol dehydrogenase [1] (data from MRSA252) NWMN_RS03425 metal ABC transporter substrate-binding protein [1] (data from MRSA252) NWMN_RS04205 triose-phosphate isomerase [1] (data from MRSA252) NWMN_RS04210 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [1] (data from MRSA252) NWMN_RS04730 hypothetical protein [1] (data from MRSA252) NWMN_RS04935 enoyl-ACP reductase [1] (data from MRSA252) NWMN_RS04955 hypothetical protein [1] (data from MRSA252) NWMN_RS05220 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase [1] (data from MRSA252) NWMN_RS05395 dihydrolipoyl dehydrogenase [1] (data from MRSA252) NWMN_RS06490 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_RS06515 succinyl-CoA ligase subunit beta [1] (data from MRSA252) NWMN_RS06575 30S ribosomal protein S2 [1] (data from MRSA252) NWMN_RS06615 proline--tRNA ligase [1] (data from MRSA252) NWMN_RS06840 glutamine synthetase [1] (data from MRSA252) NWMN_RS07120 aconitate hydratase [1] (data from MRSA252) NWMN_RS07455 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [1] (data from MRSA252) NWMN_RS07800 30S ribosomal protein S1 [1] (data from MRSA252) NWMN_RS08350 molecular chaperone DnaK [1] (data from MRSA252) NWMN_RS08675 50S ribosomal protein L27 [1] (data from MRSA252) NWMN_RS08685 50S ribosomal protein L21 [1] (data from MRSA252) NWMN_RS08795 trigger factor [1] (data from MRSA252) NWMN_RS08825 50S ribosomal protein L35 [1] (data from MRSA252) NWMN_RS08865 aldehyde dehydrogenase [1] (data from MRSA252) NWMN_RS08905 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) NWMN_RS08930 pyruvate kinase [1] (data from MRSA252) NWMN_RS08995 universal stress protein UspA [1] (data from MRSA252) NWMN_RS09000 acetate kinase [1] (data from MRSA252) NWMN_RS09045 30S ribosomal protein S4 [1] (data from MRSA252) NWMN_RS09165 hypothetical protein [1] (data from MRSA252) NWMN_RS10520 non-heme ferritin [1] (data from MRSA252) NWMN_RS10560 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B [1] (data from MRSA252) NWMN_RS11175 molecular chaperone GroEL [1] (data from MRSA252) NWMN_RS11495 D-alanine--D-alanine ligase [1] (data from MRSA252) NWMN_RS11705 aldehyde dehydrogenase family protein [1] (data from MRSA252) NWMN_RS11715 UDP-N-acetylglucosamine 1-carboxyvinyltransferase [1] (data from MRSA252) NWMN_RS11875 glutamine--fructose-6-phosphate aminotransferase [1] (data from MRSA252) NWMN_RS12080 Asp23/Gls24 family envelope stress response protein [1] (data from MRSA252) NWMN_RS12260 30S ribosomal protein S9 [1] (data from MRSA252) NWMN_RS12265 50S ribosomal protein L13 [1] (data from MRSA252) NWMN_RS12295 DNA-directed RNA polymerase subunit alpha [1] (data from MRSA252) NWMN_RS12300 30S ribosomal protein S11 [1] (data from MRSA252) NWMN_RS12305 30S ribosomal protein S13 [1] (data from MRSA252) NWMN_RS12330 50S ribosomal protein L15 [1] (data from MRSA252) NWMN_RS12345 50S ribosomal protein L18 [1] (data from MRSA252) NWMN_RS12355 30S ribosomal protein S8 [1] (data from MRSA252) NWMN_RS12365 50S ribosomal protein L5 [1] (data from MRSA252) NWMN_RS12370 50S ribosomal protein L24 [1] (data from MRSA252) NWMN_RS12380 30S ribosomal protein S17 [1] (data from MRSA252) NWMN_RS12395 30S ribosomal protein S3 [1] (data from MRSA252) NWMN_RS12400 50S ribosomal protein L22 [1] (data from MRSA252) NWMN_RS12405 30S ribosomal protein S19 [1] (data from MRSA252) NWMN_RS12410 50S ribosomal protein L2 [1] (data from MRSA252) NWMN_RS12415 50S ribosomal protein L23 [1] (data from MRSA252) NWMN_RS12420 50S ribosomal protein L4 [1] (data from MRSA252) NWMN_RS12425 50S ribosomal protein L3 [1] (data from MRSA252) NWMN_RS14370 malate:quinone oxidoreductase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: Fur* see NWMN_0617
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 1.66 1.67 1.68 1.69 1.70 1.71 1.72 1.73 1.74 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)