PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2147 [new locus tag: SA_RS12335 ]
• pan locus tag^?: SAUPAN005864000
• symbol: tcaR
• pan gene symbol^?: tcaR
• synonym:
• product: TcaR transcription regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2147 [new locus tag: SA_RS12335 ]
• symbol: tcaR
• product: TcaR transcription regulator
• replicon: chromosome
• strand: -
• coordinates: 2413209..2413664
• length: 456
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125075 NCBI
• RefSeq: NP_375470 NCBI
• BioCyc: see SA_RS12335
• MicrobesOnline: 104496 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGTAAAACATTTACAAGACCATATTCAATTTTTAGAGCAGTTTATAAATAACGTTAAC
  GCATTAACTGCAAAAATGTTGAAAGATTTACAAAATGAATATGAAATTTCATTAGAGCAG
  TCTAACGTATTAGGTATGTTAAATAAAGAACCTTTGACAATTAGTGAAATCACGCAAAGA
  CAAGGAGTAAATAAGGCCGCAGTAAGCCGACGAATTAAAAAGTTAATCGATGCTAAATTA
  GTTAAGTTAGATAAACCAAATTTAAATATTGATCAACGTTTGAAATTCATAACCTTAACT
  GACAAAGGTAGAGCATATTTGAAAGAACGTAATGCGATTATGACAGATATTGCGCAAGAT
  ATTACTAATGATTTAAATTCTGAAGATATTGAAAATGTAAGGCAAGTATTAGAAGTCATC
  AATCATCGCATTAAAACATATAGCAATCATAAATAG
  60
  120
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  456
  

⊟Protein

⊟General

• locus tag: SA2147 [new locus tag: SA_RS12335 ]
• symbol: TcaR
• description: TcaR transcription regulator
• length: 151
• theoretical pI: 9.72614
• theoretical MW: 17521.1
• GRAVY: -0.525828

⊟Function

• TIGRFAM:
  homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 27.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD family protein (TIGR04548; HMM-score: 23)
  and 4 more
  mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 15.3)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 14)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, Acidobacterial, PadR-family (TIGR03433; HMM-score: 14)
  Cellular processes Cellular processes Sporulation and germination stage II sporulation protein E (TIGR02865; EC 3.1.3.16; HMM-score: 7.9)
• TheSEED  :
  • Teicoplanin-resistance associated HTH-type transcriptional regulator TcaR
  Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Teicoplanin-resistance associated HTH-type transcriptional regulator TcaR
  and 1 more
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Teicoplanin-resistance in Staphylococcus  Teicoplanin-resistance associated HTH-type transcriptional regulator TcaR
• PFAM:
  HTH (CL0123) MarR_2; MarR family (PF12802; HMM-score: 39.4)
  and 16 more
  HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 26.8)
  HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 25.7)
  HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 25)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 23.2)
  HTH_11; HTH domain (PF08279; HMM-score: 22.5)
  MarR; MarR family (PF01047; HMM-score: 22.4)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 20.1)
  Rrf2; Transcriptional regulator (PF02082; HMM-score: 19.5)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 19)
  HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 18.7)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 15.4)
  UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 15.2)
  Peptidase_CD (CL0093) Peptidase_C11; Clostripain family (PF03415; HMM-score: 15)
  HTH (CL0123) HTH_45; Winged helix-turn-helix (PF14947; HMM-score: 14.1)
  Globin (CL0090) Protoglobin; Protoglobin (PF11563; HMM-score: 13.4)
  no clan defined ZapB; Cell division protein ZapB (PF06005; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002355
  • TAT(Tat/SPI): 0.000213
  • LIPO(Sec/SPII): 0.000279
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927937 NCBI
• RefSeq: NP_375470 NCBI
• UniProt: A0A0H3JN91 UniProt

⊟Protein sequence

• MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVLGMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDKGRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications