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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS14570 [old locus tag: SAUSA300_2622 ]
- pan locus tag?: SAUPAN006446000
- symbol: SAUSA300_RS14570
- pan gene symbol?: —
- synonym:
- product: sulfurtransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS14570 [old locus tag: SAUSA300_2622 ]
- symbol: SAUSA300_RS14570
- product: sulfurtransferase
- replicon: chromosome
- strand: +
- coordinates: 2848909..2849865
- length: 957
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2848909..2849865) NCBI
- BioCyc: SAUSA300_RS14570 BioCyc
- MicrobesOnline: see SAUSA300_2622
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGAACTATCAAGTTCTTTTATATTATAAATATATGACGATTGATGACCCTGAACAGTTT
GCTCAGGATCACTTAGCCTTTTGTAAAGCACACCATTTAAAAGGTAGAATTCTTGTTTCT
ACAGAAGGTATTAACGGCACATTATCTGGTACAAAAGAAGAAACCGAACAATATATGGCA
CATATGCATGCCGATGAACGATTCAAAGATATGGTGTTTAAAATTGATGAAGCTGAAGGA
CATGCTTTTAAGAAAATGCATGTACGTCCTCGAAAAGAAATCGTTGCTTTAGATTTAGAA
GATGACGTCGATCCAAGACACACAACTGGCCAATATTTATCACCTGTAGAATTTAGAAAA
GCTCTTGAAGATGATGACACAGTCATTATTGATGCACGTAATGATTATGAATTTGATTTA
GGTCATTTCCGAGGTGCAATTCGTCCAAATATCACACGTTTTAGAGATTTGCCTGACTGG
ATTAAAGAGAATAAAGCGTTATTTGCAGATAAAAAAGTGGTTACGTACTGTACTGGTGGC
ATTCGATGCGAAAAATTTTCTGGATGGCTTTTAAAAGAAGGTTTCGAAGATGTAGCTCAA
CTTCATGGCGGTATTGCTACATATGGTAAAGATCCTGAAACAAAAGGTGAATATTGGGAC
GGTAAAATGTACGTATTTGATGACCGTATCAGTGTTGATATCAACCAAGTTGAAAAAACA
ATTATTGGTAAGGATTGGTTTGATGGCAAACCATGTGAACGTTATATTAATTGCGCTAAC
CCAGAATGTAATAAACAAATATTAGTTTCTGAAGAAAACGAAACTAAATATTTAGGTGCA
TGCTCTTATGAATGTGCTAAACATGAGCGTAATCGTTATGTTCAAGCAAATAATATTAGT
GATAATGAGTGGCAACAACGTTTAACAAACTTTGATGATTTACATCAACATGCTTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS14570 [old locus tag: SAUSA300_2622 ]
- symbol: SAUSA300_RS14570
- description: sulfurtransferase
- length: 318
- theoretical pI: 5.18797
- theoretical MW: 37057.3
- GRAVY: -0.745597
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 18)Cys-rich protein, LA_1312 family (TIGR04453; HMM-score: 15.3)and 1 moreDNA metabolism DNA replication, recombination, and repair replication factor-a protein 1 (rpa1) (TIGR00617; HMM-score: 4.3)
- TheSEED: see SAUSA300_2622
- PFAM: no clan defined Rhodanese_C; Rhodanase C-terminal (PF12368; HMM-score: 58.5)and 3 morePhosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 44.8)OB (CL0021) Rep_fac-A_C; Replication factor-A C terminal domain (PF08646; HMM-score: 15.7)CCCH_zf (CL0537) zf-CCCH_2; RNA-binding, Nab2-type zinc finger (PF14608; HMM-score: 9.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006325
- TAT(Tat/SPI): 0.00023
- LIPO(Sec/SPII): 0.000638
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 447032017 NCBI
- RefSeq: WP_001109273 NCBI
- UniProt: see SAUSA300_2622
⊟Protein sequence[edit | edit source]
- MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGINGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDDVDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDWIKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEYWDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETKYLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS00735 2-deoxyribose-5-phosphate aldolase [1] (data from MRSA252) SAUSA300_RS02635 cysteine synthase [1] (data from MRSA252) SAUSA300_RS02700 pyridoxal 5'-phosphate synthase lyase subunit PdxS [1] (data from MRSA252) SAUSA300_RS02795 50S ribosomal protein L11 [1] (data from MRSA252) SAUSA300_RS02805 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_RS02825 DNA-directed RNA polymerase subunit beta' [1] (data from MRSA252) SAUSA300_RS02850 elongation factor Tu [1] (data from MRSA252) SAUSA300_RS03050 phosphate acetyltransferase [1] (data from MRSA252) SAUSA300_RS03850 ribonucleotide-diphosphate reductase subunit beta [1] (data from MRSA252) SAUSA300_RS04080 aldehyde dehydrogenase [1] (data from MRSA252) SAUSA300_RS04090 triose-phosphate isomerase [1] (data from MRSA252) SAUSA300_RS04560 NADH dehydrogenase [1] (data from MRSA252) SAUSA300_RS04670 glucose-6-phosphate isomerase [1] (data from MRSA252) SAUSA300_RS04925 hypothetical protein [1] (data from MRSA252) SAUSA300_RS05290 phosphocarrier protein HPr [1] (data from MRSA252) SAUSA300_RS05295 phosphoenolpyruvate--protein phosphotransferase [1] (data from MRSA252) SAUSA300_RS05620 thiol reductase thioredoxin [1] (data from MRSA252) SAUSA300_RS06080 beta-ketoacyl-ACP reductase [1] (data from MRSA252) SAUSA300_RS06165 succinyl-CoA ligase subunit alpha [1] (data from MRSA252) SAUSA300_RS06220 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_RS06230 elongation factor Ts [1] (data from MRSA252) SAUSA300_RS06485 glutamine synthetase [1] (data from MRSA252) SAUSA300_RS08950 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) SAUSA300_RS09055 2-Cys peroxiredoxin [1] (data from MRSA252) SAUSA300_RS09425 transaldolase [1] (data from MRSA252) SAUSA300_RS10250 non-heme ferritin [1] (data from MRSA252) SAUSA300_RS10900 molecular chaperone GroEL [1] (data from MRSA252) SAUSA300_RS11445 fructose-bisphosphate aldolase [1] (data from MRSA252) SAUSA300_RS11460 DNA-directed RNA polymerase subunit delta [1] (data from MRSA252) SAUSA300_RS11520 purine-nucleoside phosphorylase [1] (data from MRSA252) SAUSA300_RS11985 30S ribosomal protein S9 [1] (data from MRSA252) SAUSA300_RS12055 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12145 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_RS14100 class I fructose-bisphosphate aldolase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)