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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2481 [new locus tag: SA_RS14195 ]
  • pan locus tag?: SAUPAN006446000
  • symbol: SA2481
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2481 [new locus tag: SA_RS14195 ]
  • symbol: SA2481
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2792683..2793639
  • length: 957
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACTATCAAGTTCTTTTATATTATAAATATACGACGATTGATGACCCTGAACAGTTT
    GCTCAGGATCACTTAGCGTTTTGCAAAGCACACCATTTAAAAGGTAGAATTCTTGTTTCT
    ACAGAAGGTATTAACGGCACATTATCTGGTACAAAAGAAGAAACCGAACAATATATGGCA
    CATATGCATGCCGATGAACGATTCAAAGATATGGTGTTTAAAATTGATGAAGCTGAAGGA
    CATGCTTTTAAGAAAATGCATGTACGTCCTCGAAAAGAAATCGTTGCTTTAGATTTAGAA
    GATGACGTCGATCCAAGACACACAACTGGCCAATATTTATCACCTGTAGAATTTAGAAAA
    GCTCTTGAAGATGATGACACAGTCATTATTGATGCACGTAATGATTATGAATTTGATTTA
    GGTCATTTCCGAGGTGCAATTCGCCCAGACATCACACGCTTTAGAGATTTACCTGACTGG
    ATTAAAGAGAATAAAGCGTTATTTACAGATAAAAAAGTGGTTACGTACTGTACAGGTGGC
    ATTCGATGTGAAAAATTTTCTGGATGGCTTTTAAAAGAAGGTTTCGAAGATGTAGCGCAG
    CTTCACGGCGGTATAGCTACATATGGTAAAGACCCTGAAACAAAAGGTCAATATTGGGAC
    GGTAAAATGTATGTATTTGATGATCGTATCAGTGTTGATATCAACCAAGTTGAAAAAACA
    ATTATTGGTAAGGATTGGTTTGATGGCAAACCATGTGAACGTTATATTAATTGCGCTAAC
    CCAGAATGTAATAAACAAATATTAGTTTCTGAAGAAAACGAAACTAAATATTTAGGTGCA
    TGCTCTTATGAATGTGCTAAACATGAGCGTAATCGTTATGTTCAAGCAAATAATATTAGT
    GATAATGAGTGGCAACAACGTTTAACAAACTTTGATGATTTACATCAACATGCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2481 [new locus tag: SA_RS14195 ]
  • symbol: SA2481
  • description: hypothetical protein
  • length: 318
  • theoretical pI: 5.18509
  • theoretical MW: 37057.2
  • GRAVY: -0.761635

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 17.6)
    Cys-rich protein, LA_1312 family (TIGR04453; HMM-score: 15.3)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair replication factor-a protein 1 (rpa1) (TIGR00617; HMM-score: 3.9)
  • TheSEED  :
    • tRNA 5-hydroxyuridine(34) synthetase TrhO
  • PFAM:
    no clan defined Rhodanese_C; Rhodanase C-terminal (PF12368; HMM-score: 58.5)
    and 3 more
    Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 44.7)
    OB (CL0021) Rep_fac-A_C; Replication factor-A C terminal domain (PF08646; HMM-score: 15.7)
    CCCH_zf (CL0537) zf-CCCH_2; RNA-binding, Nab2-type zinc finger (PF14608; HMM-score: 9.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006711
    • TAT(Tat/SPI): 0.000249
    • LIPO(Sec/SPII): 0.000676
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGINGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDDVDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPDITRFRDLPDWIKENKALFTDKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQYWDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETKYLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1517(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    SA0471(cysK)hypothetical protein  [1] (data from MRSA252)
    SA1940(deoD)purine nucleoside phosphorylase  [1] (data from MRSA252)
    SA0133(dra)deoxyribose-phosphate aldolase  [1] (data from MRSA252)
    SA0545(eutD)phosphotransacetylase  [1] (data from MRSA252)
    SA1074(fabG)3-oxoacyl-ACP reductase  [1] (data from MRSA252)
    SA1927(fbaA)fructose-bisphosphate aldolase  [1] (data from MRSA252)
    SA0727(gap)glyceraldehyde-3-phosphate dehydrogenase  [1] (data from MRSA252)
    SA1150(glnA)glutamine-ammonia ligase  [1] (data from MRSA252)
    SA1836(groEL)molecular chaperone GroEL  [1] (data from MRSA252)
    SA0687(nrdF)ribonucleotide-diphosphate reductase subunit beta  [1] (data from MRSA252)
    SA0823(pgi)glucose-6-phosphate isomerase  [1] (data from MRSA252)
    SA0934(ptsH)phosphocarrier protein HPr  [1] (data from MRSA252)
    SA0935(ptsI)phosphoenolpyruvate-protein phosphatase  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SA0495(rplK)50S ribosomal protein L11  [1] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SA0501(rpoC)DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    SA1930(rpoE)DNA-directed RNA polymerase subunit delta  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA2016(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    SA1089(sucD)succinyl-CoA synthetase subunit alpha  [1] (data from MRSA252)
    SA0729(tpiA)triosephosphate isomerase  [1] (data from MRSA252)
    SA1535(tpx)thiol peroxidase  [1] (data from MRSA252)
    SA0992(trxA)thioredoxin  [1] (data from MRSA252)
    SA1100(tsf)elongation factor Ts  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA0477pyridoxal biosynthesis lyase PdxS  [1] (data from MRSA252)
    SA0802hypothetical protein  [1] (data from MRSA252)
    SA0873hypothetical protein  [1] (data from MRSA252)
    SA1599translaldolase  [1] (data from MRSA252)
    SA1709hypothetical protein  [1] (data from MRSA252)
    SA2399fructose-1,6-bisphosphate aldolase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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