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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0454 [new locus tag: NWMN_RS02595 ]
  • pan locus tag?: SAUPAN002228000
  • symbol: NWMN_0454
  • pan gene symbol?:
  • synonym:
  • product: TatD related DNase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0454 [new locus tag: NWMN_RS02595 ]
  • symbol: NWMN_0454
  • product: TatD related DNase
  • replicon: chromosome
  • strand: +
  • coordinates: 517666..518439
  • length: 774
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTTAATCGATACACATGTCCATTTAAATGATGAGCAATACGATGATGATTTGAGTGAA
    GTGATTACACGTGCTAGAGAAGCAGGTGTTGATCGTATGTTTGTAGTTGGTTTTAACAAA
    TCGACAATTGAACGCGCGATGAAATTAATCGATGAGTATGATTTTTTATATGGCATTATC
    GGTTGGCATCCAGTTGACGCAATTGATTTTACAGAAGAACACTTGGAATGGATTGAATCT
    TTAGCTCAGCATCCAAAAGTGATTGGTATTGGTGAAATGGGATTAGATTATCACTGGGAT
    AAATCTCCTGCAGATGTTCAAAAGGAAGTTTTTAGAAAGCAAATTGCTTTAGCTAAGCGT
    TTGAAGTTACCAATTATCATTCATAACCGTGAAGCAACTCAAGACTGTATCGATATCTTA
    TTGGAGGAGCATGCTGAAGAGGTAGGCGGGATTATGCATAGCTTTAGTGGTTCTCCAGAA
    ATTGCAGATATTGTAACTAATAAGCTGAATTTTTATATTTCATTAGGTGGACCTGTGACA
    TTTAAAAATGCTAAACAGCCTAAAGAAGTTGCTAAGCATGTGTCAATGGAGCGTTTGCTA
    GTTGAAACCGATGCACCGTATCTTTCGCCACATCCGTATAGAGGGAAGCGAAATGAACCG
    GCGAGAGTAACTTTAGTAGCTGAACAAATTGCTGAATTAAAAGGCTTATCTTATGAAGAA
    GTGTGCGAACAAACAACTAAAAATGCAGAGAAATTGTTTAATTTAAATTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    774

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0454 [new locus tag: NWMN_RS02595 ]
  • symbol: NWMN_0454
  • description: TatD related DNase
  • length: 257
  • theoretical pI: 4.90143
  • theoretical MW: 29281.1
  • GRAVY: -0.350584

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityhydrolase, TatD family (TIGR00010; HMM-score: 304)
  • TheSEED:  
    YcfH Uncharacterized metal-dependent hydrolase YcfH 
  • PFAM:
    Amidohydrolase (CL0034) TatD_DNase; TatD related DNase (PF01026; HMM-score: 279)
    Amidohydro_2; Amidohydrolase (PF04909; HMM-score: 20.7)
    Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 18.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0741(gapA)glyceraldehyde 3-phosphate dehydrogenase 1  [1] (data from MRSA252)
    NWMN_1217(glnA)glutamine synthetase  [1] (data from MRSA252)
    NWMN_0176(ldh1)L-lactate dehydrogenase  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_0162(pflB)formate acetyltransferase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2151(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_0839fumarylacetoacetate hydrolase family protein  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)
    NWMN_2504malate:quinone oxidoreductase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.083
    • Ymax_pos: 46
    • Cmax: 0.118
    • Cmax_pos: 68
    • Smax: 0.099
    • Smax_pos: 45
    • Smean: 0.057
    • D: 0.073
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIERAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKSPADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPEIADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRNEPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]