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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0950 [new locus tag: NWMN_RS05325 ]
  • pan locus tag?: SAUPAN003304000
  • symbol: NWMN_0950
  • pan gene symbol?: ptsI
  • synonym:
  • product: phosphoenolpyruvate-protein phosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0950 [new locus tag: NWMN_RS05325 ]
  • symbol: NWMN_0950
  • product: phosphoenolpyruvate-protein phosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 1055101..1056819
  • length: 1719
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCTAAATTAATTAAAGGTATTGCCGCATCTGATGGTGTCGCAATTGCTAAAGCTTAT
    TTATTAGTTGAGCCAGACTTAACATTCGACAAAAATGAAAAAGTCACTGATGTTGAAGGA
    GAAGTTGCAAAGTTCAATAGCGCTATCGAAGCTTCTAAAGTTGAGTTAACTAAAATTAGA
    AATAATGCAGAGGTTCAACTAGGTGCTGATAAAGCTGCTATCTTTGATGCACATTTATTA
    GTTTTAGATGACCCTGAATTAATTCAACCAATCCAAGATAAGATTAAAAATGAAAACGCT
    AATGCTGCTACAGCATTAACGGATGTAACAACACAATTTGTTACAATTTTTGAATCTATG
    GATAACGAATACATGAAAGAACGTGCGGCTGATATTCGCGACGTTTCTAAACGTGTGTTA
    TCACATATTTTAGGTGTAGAATTACCGAATCCGAGTATGATTGATGAAAGCGTTGTTATT
    GTAGGGAATGACTTAACGCCATCTGATACTGCTCAATTAAATAAAGAATTCGTACAAGGT
    TTTGCTACAAACATTGGCGGAAGAACAAGTCACTCTGCAATTATGAGTCGTTCTTTAGAA
    ATTCCAGCAATTGTTGGTACAAAATCAATTACTCAAGAAGTTAAACAAGGCGACATGATT
    ATCGTAGATGGATTAAATGGTGATGTAATCGTTAATCCAACTGAAGATGAGTTAATCGCT
    TATCAAGATAAACGTGAGCGTTATTTTGCTGACAAGAAAGAATTACAAAAACTACGTGAT
    GCTGATACTGTTACAGTTGATGGTGTTCACGCAGAGCTTGCTGCAAATATTGGTACACCT
    AATGATTTGCCAGGTGTTATTGAAAATGGTGCACAAGGTATCGGCTTATATAGAACTGAG
    TTTTTATATATGGGTCGTGACCAAATGCCTACAGAAGAAGAACAATTTGAAGCTTATAAA
    GAAGTATTAGAAGCAATGGGCGGTAAACGTGTTGTTGTACGTACTTTAGATATAGGTGGA
    GATAAAGAATTATCATACTTAAACTTGCCTGAAGAAATGAATCCATTCTTAGGTTACCGT
    GCGATTCGTTTATGCCTTGCGCAACAAGATATTTTCAGACCACAGCTACGTGCATTATTA
    CGTGCATCAGTTTATGGTAAGTTAAATATCATGTTCCCAATGGTTGCAACAATTAACGAA
    TTTAGAGAAGCTAAAGCTATATTATTAGAAGAAAAAGAAAACCTTAAAAATGAAGGTCAT
    GACATTTCGGATGATATAGAATTAGGAATCATGGTAGAGATACCTGCAACAGCAGCATTA
    GCTGATGTCTTTGCTAAAGAAGTAGATTTCTTCAGTATCGGTACAAATGATTTAATTCAA
    TACACATTAGCTGCTGACCGTATGTCAGAGCGTGTATCATATCTATACCAACCATATAAC
    CCTTCAATCTTACGTTTAGTTAAACAAGTTATTGAAGCGTCACATAAAGAAGGTAAATGG
    ACAGGTATGTGTGGTGAAATGGCTGGAGATGAAACAGCTATTCCATTATTGCTTGGTTTA
    GGTTTAGATGAGTTCTCTATGAGTGCAACGTCTATTCTGAAAGCAAGAAGACAAATTAAT
    GGTTTAAGTAAAAATGAAATGACTGAACTTGCTAACCGTGCAGTCGACTGTGCAACGCAA
    GAAGAAGTTATTGAATTAGTTAACAACTACGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1719

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0950 [new locus tag: NWMN_RS05325 ]
  • symbol: NWMN_0950
  • description: phosphoenolpyruvate-protein phosphatase
  • length: 572
  • theoretical pI: 4.38638
  • theoretical MW: 63218.6
  • GRAVY: -0.174126

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.3.9?  ExPASy
    Phosphoenolpyruvate--protein phosphotransferase Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
  • TIGRFAM:
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 816.2)
    and 2 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 209.3)
    Metabolism Energy metabolism Other pyruvate, phosphate dikinase (TIGR01828; EC 2.7.9.1; HMM-score: 135.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PK_TIM (CL0151) PEP-utilizers_C; PEP-utilising enzyme, TIM barrel domain (PF02896; HMM-score: 426.5)
    and 2 more
    no clan defined PEP-utilisers_N; PEP-utilising enzyme, N-terminal (PF05524; HMM-score: 111.9)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 83.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010447
    • TAT(Tat/SPI): 0.000492
    • LIPO(Sec/SPII): 0.00181
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIELVNNYVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_1605(ackA)acetate kinase  [1] (data from MRSA252)
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_0002(dnaN)DNA polymerase III subunit beta  [1] (data from MRSA252)
    NWMN_1096(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    NWMN_2315(gpmA)phosphoglyceromutase  [1] (data from MRSA252)
    NWMN_1178(infB)translation initiation factor IF-2  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_2040(pdp)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    NWMN_0162(pflB)formate acetyltransferase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0960(phdB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2152(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    NWMN_2151(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_0499(rplK)50S ribosomal protein L11  [1] (data from MRSA252)
    NWMN_0502(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_2120(rplM)50S ribosomal protein L13  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_2136(rplR)50S ribosomal protein L18  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1572(rplT)50S ribosomal protein L20  [1] (data from MRSA252)
    NWMN_1549(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_1134(rpmB)50S ribosomal protein L28  [1] (data from MRSA252)
    NWMN_2024(rpmE2)50S ribosomal protein L31 type B  [1] (data from MRSA252)
    NWMN_2126(rpoA)DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0508(rpsG)30S ribosomal protein S7  [1] (data from MRSA252)
    NWMN_2138(rpsH)30S ribosomal protein S8  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_2127(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    NWMN_2128(rpsM)30S ribosomal protein S13  [1] (data from MRSA252)
    NWMN_1148(rpsP)30S ribosomal protein S16  [1] (data from MRSA252)
    NWMN_2143(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    NWMN_0359(rpsR)30S ribosomal protein S18  [1] (data from MRSA252)
    NWMN_2148(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    NWMN_1569(tig)trigger factor  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_2016(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    NWMN_0601hypothetical protein  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)
    NWMN_24541-pyrroline-5-carboxylate dehydrogenase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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